[ccp4bb] OT: Ribosomal processivity

2013-09-24 Thread Katherine Sippel
Denizens of the bulletin board,

I would like to tap into your vast knowledge of all things protein
expression. Is anyone aware of a cell line or even a
transformable/transfectable construct that produces high processivity
ribosomes. I.E. one that can translate to the end of difficult mRNA without
aborting prematurely? It doesn't have to be for E. coli. Yeast or Insect
cells would be acceptable too.

Any help would be appreciated.

Cheers,
Katherine

-- 
Nil illegitimo carborundum* - *Didactylos


Re: [ccp4bb] OT: Ribosomal processivity

2013-09-24 Thread Katherine Sippel
I forgot to mention that in this case gene optimization and eukaryotic
expression don't help. It's a product of the protein sequence itself.


On Tue, Sep 24, 2013 at 10:29 AM, Katherine Sippel 
katherine.sip...@gmail.com wrote:

 Denizens of the bulletin board,

 I would like to tap into your vast knowledge of all things protein
 expression. Is anyone aware of a cell line or even a
 transformable/transfectable construct that produces high processivity
 ribosomes. I.E. one that can translate to the end of difficult mRNA without
 aborting prematurely? It doesn't have to be for E. coli. Yeast or Insect
 cells would be acceptable too.

 Any help would be appreciated.

 Cheers,
 Katherine

 --
 Nil illegitimo carborundum* - *Didactylos




-- 
Nil illegitimo carborundum* - *Didactylos