Re: [ccp4bb] is small dialysis tubes reusable??
If cost is an issue, I recommend preparing your own MINI Dialysis Devices at 99.74% savings. Cut a hole in the lid of an Eppendorf tube, put your sample in the tube, then close the lid over a small piece of dialysis tubing, trapping it as a cover. Float inverted in dialysis buffer and Bob's your uncles for pennies. Andreas On 26/09/2014 4:51, Manjula Ramu wrote: Hi all, I use Slide-A-Lyzer MINI Dialysis Devices from Thermo for dialysis of small protein volumes. Are they reusable? if so how can we store them?? Thanks and Regards, Manjula R Research Scholar Department of Biophysics National Institute of Mental Health and Neurosciences Bengaluru-29, Karnataka INDIA E-mail: manjula@gmail.com mailto:manjula@gmail.com Mobile no:+91-9538553356 http://www.nimhans.kar.nic.in/ https://www.nimhans.kar.nic.in/ -- Andreas Förster Crystallization and X-ray Facility Manager Centre for Structural Biology Imperial College London
Re: [ccp4bb] is small dialysis tubes reusable??
For even smaller volumes, make a dialysis button from an eppendorf tube: With a razor blade, cut the tube off leaving only the rim that the cap snaps into. Put your sample in the depression in the middle of the cap (inside). cover with membrane and snap the rim down over it. (I have done this but never actually used it for dialysis or crystallization) For volumes .5 ml or larger, just get the pencil diameter tubing, (soak and maybe boil it), cut off the right length and use reusableclips to hold the ends. eab On 09/28/2014 01:30 PM, Andreas Förster wrote: If cost is an issue, I recommend preparing your own MINI Dialysis Devices at 99.74% savings. Cut a hole in the lid of an Eppendorf tube, put your sample in the tube, then close the lid over a small piece of dialysis tubing, trapping it as a cover. Float inverted in dialysis buffer and Bob's your uncles for pennies. Andreas On 26/09/2014 4:51, Manjula Ramu wrote: Hi all, I use Slide-A-Lyzer MINI Dialysis Devices from Thermo for dialysis of small protein volumes. Are they reusable? if so how can we store them?? Thanks and Regards, Manjula R Research Scholar Department of Biophysics National Institute of Mental Health and Neurosciences Bengaluru-29, Karnataka INDIA E-mail: manjula@gmail.com mailto:manjula@gmail.com Mobile no:+91-9538553356 http://www.nimhans.kar.nic.in/ https://www.nimhans.kar.nic.in/ -- Andreas Förster Crystallization and X-ray Facility Manager Centre for Structural Biology Imperial College London
[ccp4bb] is small dialysis tubes reusable??
Hi all, I use Slide-A-Lyzer MINI Dialysis Devices from Thermo for dialysis of small protein volumes. Are they reusable? if so how can we store them?? Thanks and Regards, Manjula R Research Scholar Department of Biophysics National Institute of Mental Health and Neurosciences Bengaluru-29, Karnataka INDIA E-mail: manjula@gmail.com Mobile no:+91-9538553356 http://www.nimhans.kar.nic.in/ https://www.nimhans.kar.nic.in/
Re: [ccp4bb] is small dialysis tubes reusable??
Hi Manjula, I imagine that they could be reusable, if you stored them in a buffer with some sort of preservative like sodium azide (NB Toxic) or maybe a bacteriostatic agent like EDTA or something. However, when you use them, a small amount of protein is going to remain in the cassette after each use, so I would only use each cassette for a specific protein to prevent cross-contamination. Additionally, I'd worry about protein from old preps falling apart/denaturing and then contaminating future fresh preps of protein. I guess it depends at what step during purification you are using them. HTH, Dave [image: David Briggs on about.me] David Briggs about.me/david_briggs http://about.me/david_briggs On 26 September 2014 15:51, Manjula Ramu manjula@gmail.com wrote: Hi all, I use Slide-A-Lyzer MINI Dialysis Devices from Thermo for dialysis of small protein volumes. Are they reusable? if so how can we store them?? Thanks and Regards, Manjula R Research Scholar Department of Biophysics National Institute of Mental Health and Neurosciences Bengaluru-29, Karnataka INDIA E-mail: manjula@gmail.com Mobile no:+91-9538553356 http://www.nimhans.kar.nic.in/ https://www.nimhans.kar.nic.in/
