Re: [ccp4bb] mosaicity and SAD
Dear Supratim, In my experience, as long as you do not run into an overlap problem, a large mosaicity is not a problem. If the statistics look good, you can safely use the data. However, if the completeness of the data is much lower than what was predicted, many spots may have been thrown out because of overlaps and you may want to correct this. There has been a recent thread about this in the CCP4BB. Also, for large mosacities, you may want to consider a processing program with 3D profile fitting like XDS. Best, Herman From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of supratim dey Sent: Friday, August 31, 2012 4:56 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] mosaicity and SAD Hi friends what is the maximum mosaicity value to work with. When i am doing Heavy metal replacement my mosaicity value is around 1 - 1.2 after processing through HKL. I don't know is it acceptable data. I have used mercuric chloride for my protein and it has shown good results .However in case of potassium hexachloroplatinate it is not absorbing at all .Can you please suggest what other heavy metals can i use.
[ccp4bb] mosaicity and SAD
Hi friends what is the maximum mosaicity value to work with. When i am doing Heavy metal replacement my mosaicity value is around 1 - 1.2 after processing through HKL. I don't know is it acceptable data. I have used mercuric chloride for my protein and it has shown good results .However in case of potassium hexachloroplatinate it is not absorbing at all .Can you please suggest what other heavy metals can i use.
Re: [ccp4bb] mosaicity and SAD
Hi friends what is the maximum mosaicity value to work with. When i am doing Heavy metal replacement my mosaicity value is around 1 - 1.2 after processing through HKL. I don't know is it acceptable data. I have used mercuric chloride for my protein and it has shown good results .However in case of potassium hexachloroplatinate it is not absorbing at all .Can you please suggest what other heavy metals can i use. -- Hi Supratim, The CCP4 wiki has a number of useful links about soaking with heavy metals: http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Soaking_with_heavy_atoms Take Care, Sean Seaver P212121 http://store.p212121.com/
