[Histonet] Control Slides

[Ken M via Histonet]

May I ask, in all your opinions, Who is supplying the best control slides for 
clinical settings? Both Special Stain and IHC? What would you like to see in 
your control slides? What don't you want to see- long lead times, quality? As 
for Research, what are your requirements?
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Re: [Histonet] Tissue falling off slides

[Ken M via Histonet]

1) Try tap water
2) Try hydrophilic IHC slides, (I will send you samples for free)

Ken
kdea...@hotmail.com


-Original Message-
From: richard cartun via Histonet 
Sent: Wednesday, January 31, 2024 10:29 AM
To: histonet@lists.utsouthwestern.edu; Foshey, Annette 
Subject: Re: [Histonet] Tissue falling off slides

 Hi Annette!
Do you put anything else in your water bath besides deionized water?
Richard CartunMorphologic Proteomics, LLC(860) 490-7633

On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via 
Histonet  wrote:


I work with whole mount prostate blocks that I cut and stain for IHC manually. 
I use superfrost plus slides that I purchase from the Brain Research 
Laboratories. I airdry the slides after sectioning and then they are placed in 
a rack and dried in a 60 degree oven overnight.  On some of the slides the 
tissue still lifts off the slides usually it is where there is tumor.  Any 
suggestions would be greatly appreciated.

Kindest Regards,
Annette Foshey, HT
Histology Technologist, Precision Medicine Laboratory (PML)
Phone: (414) 955-7537 | E-mail: afos...@mcw.edu
Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226

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[Histonet] Aspergillus

[Ken M via Histonet]

Can anyone provide me with Aspergillus tissue embedded blocks? Prefer good 
quality naturally occurring but will accept good quality home grown. We usually 
use Placenta, but the tissue doesn't matter much. Will buy or trade.

kdea...@hotmail.com
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[Histonet] IHC/Special staining

[Ken M via Histonet]

Does anyone know of any lab with excess capacity good quality and fast 
turnaround that could do IHC/special staining on the side? We are out of 
capacity, and we don't always have the antibodies that we need.

Kevin
kdea...@hotmail.com
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[Histonet] MOHS staining

[Ken M via Histonet]

For those doing MOHS staining either manually or automatically. How many 
staining cups do you really use? Are six enough if there is a running water cup 
nearby or do you need more?

Ken
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[Histonet] What they don't want you to know.

[Ken M via Histonet]

How can you make some of your Histology equipment last forever?

Water bath- fill the water every morning with water at about the same 
temperature that you operate. Every one of these water bath units contain a 
heating element that is the biggest source of failure. The less you make it 
work means you can extend the life of your unit ten times. Heat the water in a 
microwave or on the stove first.

Embedding Center- Same thing. Melt the wax ahead of time in a less draw 
intensive "coffee pot." before you put it in the chamber.  Don't ever crank the 
temperature of the unit up to melt the wax fast so you can get to work quicker. 
The heating elements will fail in three times their life.  Replacing the 
heating element in your embedding center will cost you $600-1000 dollars. 
Replacing the coffee pot $100.

Block Trimmer- same thing. Don't let it run too long when you are not using 
them. They use the exact same silicone heating element. The less it is on the 
longer it lasts.

Cryostat- put it to sleep at minus 10 each night. on weekends turn it off, open 
the top and let it dry out over the weekend. Don't believe the crap about the 
seals drying out if you turn it off. Do they dry out in your car in the Winter 
when the A/C is not running?
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[Histonet] Sections sticking to tweezers

[Ken M via Histonet]

Can anyone tell me why my sections are sticking to my tweezers and turning into 
one long string of snot when I try to separate them? I have a good pair of 
curved stainless tweezers and I am trying both the front and the back and 
tapping sharply while allowing the tweezers to open at the separation point. I 
can separate one or two, then it sticks and pulls up 7 or 8 sections into one 
long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. 
Even when I go to 6 it still does it. Any ideas?

Ken
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[Histonet] Looking for laboratory assistant

[Ken M via Histonet]

High volume, fast growing non-clinical laboratory in Durham, NC looking for a 
laboratory assistant. Some experience staining, cutting on Microtome and 
printing slides a plus, but not necessary. Willingness to learn to use the 
Biocare Oncore Pro IHC staining platform a plus. Work closely with lab manager 
on different laboratory tasks. Part time to start, but can easily be full time 
as learning increases. Ideal for retired Histotech who needs flexible hours and 
doesn't need the pressure of a clinical lab, or an at home mom with kids at 
school. Large, climate controlled, well ventilated, well lit lab that doesn't 
skimp on supplies or equipment.  Long and short term disability included. 
Regular bonuses paid as company exceeds sales targets.  Contact Dr. Nie at 
diya...@gmail.com  for more information

Nie Hui PHd, MD


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[Histonet] SV40

[Ken M via Histonet]

Does anyone have a tissue block of IHC SV40?  We can swap with you for almost 
every other block you can think of, or buy it from you. We have a partner 
desperate for it to complete a study.

Ken
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Re: [Histonet] Blade holder assitance

[Ken M via Histonet]

Charles:

Is there a spacer under the blade to convert hi to lo profile?  Is there a spot 
for one?  You may be missing, or have the wrong spacer.

Ken

From: Charles Riley via Histonet 
Sent: Friday, April 7, 2023 3:34 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] Blade holder assitance

Hello all,

I am working with a microtome that has an unusual blade holder.  Standard
low profile blades are too short and are covered by the stage so no cutting
occurs. The standard high profile blades stick out above the blade holder
edge significantly and this causes wobble in the blade while sectioning
(creating thick and thin sections and or skipping sections entirely before
cutting a really thick section).

Can anyone suggest what type of blade I should purchase to use with this
holder?  I was thinking maybe sapphire knife blades might fit but didn't
want to waste money buying items I don't need
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[Histonet] Problems with Melan A staining

[Ken M via Histonet]

One of my histotech friends is having issues with Melan A staining. Using 
MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue 
control was stained strongly positive while the positive melanoma tissue 
control was stained weakly positive. He couldn’t figure out why after several 
tries to reduce background. Can someone give me some suggestions? Thank you 
very much!


Ken

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Re: [Histonet] Expired Tissue Blocks

[Ken M via Histonet]

The law is the law in the USA. So far the unanoumous decisions across the USA 
courts have been very clear.  For those who are being super cautious despite 
the prevailing laws of the USA, You are doing very little to help develop new 
antibodies and enhance cancer research.

From: Whitaker, Bonnie via Histonet 
Sent: Thursday, January 12, 2023 9:05 PM
To: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Expired Tissue Blocks

Caution to all--make sure your patients have signed consent forms that will 
include this use of their tissue.

Bonnie Whitaker
Ohio State University
AP Operations Director

614-293-8418

-Original Message-
From: Ken M via Histonet 
Sent: Thursday, January 12, 2023 3:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Expired Tissue Blocks

External Email: CAUTION

Don't throw your expired embedded blocks in the trash. Some of them can be very 
valuable for research and antibody development. If you can provide a brief 
description of any positive block (no patient identifiers or names please), we 
will pay you top dollar to take them and send you all shipping labels and 
documents ahead of time so you can send your entire storage unit to us. We can 
provide certification that we will properly dispose of and be responsible for 
the blocks in our possession if our research partners find them of no use. For 
some specific positive tissues, a single block blocks can be worth over a 
hundred dollars to our partners.

Please contact us at contac...@springsidesci.com if interested.


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[Histonet] Tissue blocks

[Ken M via Histonet]

If that were true, all cancer research and antibody development in the USA 
would come to a complete halt. Get a signature from someone who died of cancer 
10 years ago??  Here are the findings of most US courts. Here are the facts.

In legal disputes concerning the research use of excised tissues, courts 
consistently have rejected the idea that patients are the owners of their 
excised tissues or retain any property rights. While only three cases have been 
adjudicated to date, the findings (in both state and federal courts) have been 
consistent: patients do not have individual ownership, retain property rights, 
or have complete control over research use of their excised tissues.9,

Contact your legal Counsil to find the tructh.  We do not want any patient 
identifiers or case numbers.





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[Histonet] Expired Tissue Blocks

[Ken M via Histonet]

Don't throw your expired embedded blocks in the trash. Some of them can be very 
valuable for research and antibody development. If you can provide a brief 
description of any positive block (no patient identifiers or names please), we 
will pay you top dollar to take them and send you all shipping labels and 
documents ahead of time so you can send your entire storage unit to us. We can 
provide certification that we will properly dispose of and be responsible for 
the blocks in our possession if our research partners find them of no use. For 
some specific positive tissues, a single block blocks can be worth over a 
hundred dollars to our partners.

Please contact us at contac...@springsidesci.com if interested.


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[Histonet] Tech Needed

[Ken M via Histonet]

We are looking for a part-time technician for our fast growing Durham, NC lab. 
A retired Histotech would be ideal. No live patients or grossing. Mostly 
cutting and staining processed and embedded blocks. Some IHC experience would 
be great, but not necessary.  Contact Helena at hmur...@springsidesci.com for 
details.

Ken
Springside scientific
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[Histonet] AFB blocks

[Ken M via Histonet]

Does anyone know where I can obtain good Acid Fast Bacteria tissue blocks?

Ken
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[Histonet] Acid Fast

[Ken M via Histonet]

Hi everyone- We have used a modified Ziehl Neelson stain on some breast tissue 
and found round shape organisms that are Acid Fast and not rod-like. Is anyone 
familiar with this before I send them to our Pathologist?

Ken
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[Histonet] Apple Green Birefringence in Amyliod slides

[Ken M via Histonet]

Hi everyone.  I was wondering if anyone out there has any experience with 
diagnosing Amyloid tissue using Congo Red stained Kidney using polarized 
lenses.  Is it common to use polarized light to detect Amyloid deposits?  Does 
the absence of the "apple green birefringence" indicate a problem with the 
control tissue or the control slides?  Should this green bifringence always 
appear to confirm the diagnosis?  I know that the tissue should be cut thicker 
than normal (we usually cut at 5), but in the future maybe we will cut at 7 or 
8?
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[Histonet] Gram Staining

[Ken M via Histonet]

Hi.

We are trying to stain a supposed Gram Positive paraffin Tonsil tissue block 
using Gentian Violet, Iodine (Lugol), Gram's decolorizer, Carbol Fuchsin 
Counter and Tartrazine and are having trouble distinguishing the positive from 
the negative. Anyone have any suggestions?


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[Histonet] Anyone having difficulty with tissue adhesion?

[Ken M via Histonet]

Hi everyone

We have developed a new hydrophilic IHC microscope slide that uses a new 
adhesive process that dries up to 50% faster than a normal Silane coated 
charged slide and we are looking for someone that might be using a Ventana or 
who may be dealing with difficult tissue or a difficult protocol that may be 
causing them to lose tissue who can try them for us in a difficult real world 
setting. If Anyone is interested, I can send you several packs at no charge.

Ken
kdea...@hotmail.com

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[Histonet] H Staining Protocol

[Ken M via Histonet]

Hello:

Does anyone have a good manual or automatic H staining protocol that will 
work well on multiple Paraffin embedded  tissues?

Ken
kdea...@hotmail.com

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[Histonet] Recommended thickness of Amyloid sections

[Ken M via Histonet]

Hello All.  We have always cut all of our histology control slides at 5m.  We 
were told today that it is common practice to cut Amyloid at 8m?  Is this your 
experience?

Ken
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[Histonet] Question concerning H-Pylori Staining

[Ken M via Histonet]

Hi everyone!  We have a question about staining for H-Pylori Using Quick Stain 
(Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue stock, 
Sodium Hydroxide) we notice what clearly  looks like the H-Pylori purple 
stained clusters, but after dehydration in 100% alcohol the purple clusters 
seem to disappear. Should we just dehydrate using a slide oven instead of the 
alcohol? For how long at what temp? Could the alcohol be affecting the purple 
color making it too light to see?

Michelle
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[Histonet] Paraffin Tissue Blocks

[Ken M via Histonet]

To anyone interested:

Please don't throw your old tissue blocks in the trash after they age out after 
10 years. Many of them can be used by researchers and Pathologists and are 
badly needed. If you don't want to sort them out, we will pay you a fair price 
for the whole lot as long as we have a list of what they are and your lab is 
properly certified.

Ken Marzinsky
4122 Bennett Memorial Road
Durham, NC 27712
713-822-0256
kdea...@hotmail.com
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[Histonet] Tissue Blocks

[Ken M via Histonet]

Hello All:

I have thousands of tissue blocks from a large US certified reference lab. Most 
of them were used for IHC diagnosis. I am looking to trade what I have for more 
common Histology blocks for control slides such as Mast Cell, Pneumocystis, 
Copper, Gram, Aspergillus fungus, H-Pylori, Spirochete, Acid Fast Bacteria and 
other common Histology tissue for special stain control slides. I also have 
lots of normal tissue blocks. Please let me know if you are interested.

Ken Marzinsky
4122 Bennett Memorial Road
Durham, NC 27712
713-822-0256
kdea...@hotmail.com
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