Re: [R-sig-phylo] Comparing means under a phylogenetic perspective?

2011-03-23 Thread Enrico Rezende

Rafael,
a phylogenetic paired t-test should do the job. Please check this 
reference:


Lindenfors P, Revell LJ, and Nunn CL. Sexual dimorphism in primate 
aerobic capacity: a

phylogenetic test. J Evolution Biol 23: 1183-1194, 2010.

Liam, any comments on this?
Enrico




Rafael Maia escribió:

Hello everyone,

first of all, I would like to apologize if this question is too basic. I've 
been trying to study comparative methods and their implementation in R for 
several months now, but haven't taken any formal courses on it, so maybe I'm 
missing something extremely obvious. I also apologize since this isn't 
necessarily R-related, but this seemed like a good forum to ask...

My question relates to the evolution of ornamental colors. Specifically, I am interested 
in comparing ornamental and non-ornamental colors in birds over a range of species, in 
order to test if ornamental colors (trait A) are showier than non-ornamental 
ones (trait B). Both traits are measured in the same scale, and are in the same unit.

From what I understand of independent contrasts, the independence of data points is achieved by obtaining phylogenetically independent values of the change of these traits in a node. This makes a lot of sense to me if you are asking a question of if these traits are evolving in a correlated fashion, so that you'd expect a change in one of them to be accompanied by a change in the other. 


However, this is not necessarily what I am interested in. I would actually like 
to know if the changes in these traits occur in a manner in which, even if they 
are changing independently, trait A is *always larger* than trait B. So, for 
example, trait A might remain very similar between two species (i.e. low 
contrast), whereas trait B changes a lot (high contrast), but trait B is still 
lower than trait A in both species. Thus, a correlation of contrasts does not 
seem to tackle my issue. In a similar manner, comparing contrasts of both 
traits under a t-test does not seem to do the trick either, since contrasts 
will reflect the variation in the variables over the phylogeny, and as I said, 
this does not seem to be a question of how the variations in the variables are 
associated...

after banging my head against the wall for weeks with this issue, I'm not even sure if 
this question should actually be asked in a phylogenetic context - would simply comparing 
the ratio of trait A x trait B across these species suffice? It doesn't seem so, since 
common descent is expected to play a role in their evolution. However, I am not sure 
anymore what is the right question to address my hypothesis anymore...

Any thoughts would be deeply appreciated.

Many thanks in advance!

Abraços,
Rafael Maia
---
webpage: http://gozips.uakron.edu/~rm72
A little learning is a dangerous thing; drink deep, or taste not the Pierian 
spring. (A. Pope)
Graduate Student - Integrated Bioscience
University of Akron
http://gozips.uakron.edu/~shawkey/
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--
***
Enrico L. Rezende

Departament de Genètica i de Microbiologia
Facultat de Biociències, Edifici Cn
Universitat Autònoma de Barcelona
08193 Bellaterra (Barcelona)
SPAIN

Telephone: +34 93 581 4705
Fax: +34 93 581 2387
E-mail:enrico.reze...@uab.cat

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[R-sig-phylo] Conditioning on Total Tips in Birth-Death Trees

2011-03-23 Thread David Bapst
Hello all,
Are there any birth-death tree functions which condition on the total
number of tips (extinct and extant) on a tree rather than the number
of surviving tips? All the ones I've been able to find condition on
time or on the number of extant tips. I put together the following
kludge using a while() loop and the rlineage() function in ape, which
returns bd trees within a given range of total number of tips, but it
would be ideal to me not to have to condition on time.

bdtree.paleo-function(birth,death,time,minTip=0,maxTip=Inf){
rt-rlineage(birth,death,Tmax=time)
while(Ntip(rt)minTip | 
Ntip(rt)maxTip){rt-rlineage(birth,death,Tmax=time)}
rt
}

Also, I'm not very familiar with the birth-death literature and I've
been looking for one particular bit of information, which I thought I
might ask if anyone here knew. I was wondering if it was known what
the expected probability distribution of branch lengths for a
fully-sampled phylogeny (includes all extinct lineages) is? I've been
trying to figure out, if for a fully extinct tree with homogenous
rates,  whether we would expect the branch lengths of internal
branches to follow an exponential distribution based on the birth
parameter (and extinct terminal edges following an exponential
distribution based on the death parameter) or if the branch lengths
are a function of both parameters. Perhaps I'm not using the right
keywords, but my literature searches haven't found the information I'm
looking for.

Thanks,
-Dave

-- 
David Bapst
Dept of Geophysical Sciences
University of Chicago
5734 S. Ellis
Chicago, IL 60637
http://home.uchicago.edu/~dwbapst/

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Re: [R-sig-phylo] How to detect phylogenetic signal (lambda) in one unscaled trait?

2011-03-23 Thread tgarland
Hi Alberto,

OK, I think the bigger and more general issue is how to correct for 
correlations with body size.  this is an issue in many circumstances, both 
biologically and statistically, aside from any questions about how best to test 
for or quantify phylogenetic signal.  I think that if you get it right from the 
biological/statistical perspective then you will also have the answer regarding 
what to do for phylogenetic signal.

When you compute a ratio (divide by a measure of body size) in hopes of 
removing the effects of body size you are implicitly assuming that the trait 
varies directly with the measure of body size.  For example, computing tail 
length/body length for snakes might be a good way to remove the effects of 
body size if snakes of all sizes, on average, have tails of the same relative 
length.

However, many traits do not vary directly with any measure of body size.  For 
example, resting metabolic rate generally does not vary directly with body 
mass.  Rather, it scales on body mass (log-log plot) with a slope of about 
0.6-0.8.  Hence, if you compute the ratio metabolic rate/body mass it will show 
a negative relation with body mass, and so you have not removed the 
correlation with body mass.  In such a case, it is generally better to do the 
log-log regression and compute residuals.  Or, you can do what I described 
previously (see Blomberg et al., 2003, pages 720-721).

In some cases, you might have strong a priori knowledge or particular 
biological needs that would lead you to trust computing a ratio for your 
particular purposes.  For example, one might compute the ratio of forelimb 
length divided by hindlimb length of lizards for some purposes.  In general, 
however, the regression approach is probably safest if you want to then analyze 
a trait that is no longer correlated with body size.

Cheers,
Ted

 
 Original message 

  Date: Wed, 23 Mar 2011 02:13:36 +0200
  From: Alberto Gallano alberto@gmail.com
  Subject: Re: [R-sig-phylo] How to detect phylogenetic signal
  (lambda) in one unscaled trait?
  To: Alejandro Gonzalez alejandro.gonza...@ebd.csic.es
  Cc: R-phylo Mailing-list r-sig-phylo@r-project.org

  Thanks Alejandro,
  
  yes, I see this difference. I think my question is: if the goal is
  to assess
  phylogenetic signal in a trait, after accounting for interspecific
  differences in body size, which of these two alternatives is
  preferable?
  They both seem to calculate lambda after correcting for body size.
  Is one
  way more correct, given the above stated goal?
  
  regards,
  
  Alberto
  
  
  
  On Wed, Mar 23, 2011 at 1:53 AM, Alejandro Gonzalez 
  alejandro.gonza...@ebd.csic.es wrote:
  
   Hi Alberto,
  
   The results differ between the two approaches because you're
  actually
   estimating two different things.
  
   gls(logY ~ logX, correlation=corPagel(1, tree), method=ML)
  
  
   Will give you the estimate of lambda for the residuals of the
  fitted model.
  
   while:
  
   fitContinuous(tree, log(Y/X), model=lambda)
  
  
   will give you the lambda value of the ratio of the two traits.
  
  
   Cheers,
  
   Alejandro
  
   On 23, Mar 2011, at 12:47 AM, Alberto Gallano wrote:
  
   Thanks Ted and Joe, that helps a lot with my understanding.
  
  
   Given then that the variables should be on a log scale, as you
  suggest, is
   there any reason to chose a regression model estimate of lambda:
  
   gls(logY ~ logX, correlation=corPagel(1, tree), method=ML)
  
   where X is a body size proxy (i.e., scaling is done in the
  model), over a
   ratio approach?:
  
   fitContinuous(tree, log(Y/X), model=lambda)
  
   These seem to produce different results. Is there a preference
  for one
   other
   the other in a comparative methods context? Or is this just a
  question of
   whether one prefers to size 'correct' using ratios vs residuals?
  
   kind regards,
  
   Alberto
  
  
   On Wed, Mar 23, 2011 at 1:30 AM, Joe Felsenstein
  j...@gs.washington.edu
   wrote:
  
  
   Ted wrote:
  
  
   Following on that, various papers (I can't remember the
  references)
  
   have argued that imagining Brownian-like evolution of body size
  on a
  
   log scale seems reasonable. That is, it should be equally easy
  for an
  
   elephant's body size to evolve 10% as for a mouse's body size to
  
   evolve 10%, and to analyze that you want everybody on a log
  scale.
  
   Extending this, you would want to use log(Y/X) or log(Y/[X raised
  to
  
   some allometric slope]).
  
  
   It's just easier to put all variables onto their log scales, so
  you
  
   have log(X), log(Y), log(Z) and then the allometric stuff just
  
   corresponds to linear combinations there, which you already have
  
   machinery to do.
  
  
   The recommendation to use log scales is a very old one: I talk
  
   about it in my Theoretical Evolutionary Genetics free e-text.
  
   But is older than that. Falconer has a whole chapter on Scale
  
   in his 1960 Introduction 

Re: [R-sig-phylo] Conditioning on Total Tips in Birth-Death Trees

2011-03-23 Thread Dan Rabosky

Hi Dave-


 Are there any birth-death tree functions which condition on the total
 number of tips (extinct and extant) on a tree rather than the number
 of surviving tips?

You can recode the birthdeath.tree function from Geiger to do this if you want. 
The total number of species in the tree (living plus extinct) is equal to 
nrow(edge)/2, where edge is the edge matrix. Find the repeat statement and 
replace the line:

if (taxa.stop) 
if (sum(alive) = taxa.stop) 
  break

with the following:

if (taxa.stop)
if ((nrow(edge)/2) = taxa.stop)
break;

The new birthdeath.tree function should stop when taxa.stop lineages have been 
born (regardless of whether they've subsequently gone extinct).

  I was wondering if it was known what
 the expected probability distribution of branch lengths for a
 fully-sampled phylogeny (includes all extinct lineages) is?

If you have a perfectly sampled tree, branches should be exponentially 
distributed with rate (lambda + mu). Events occur in the birth-death process 
with rate (lambda + mu), and the probability that a given event will be 
speciation is lambda/(lambda + mu).

~Dan



 I've been
 trying to figure out, if for a fully extinct tree with homogenous
 rates,  whether we would expect the branch lengths of internal
 branches to follow an exponential distribution based on the birth
 parameter (and extinct terminal edges following an exponential
 distribution based on the death parameter) or if the branch lengths
 are a function of both parameters. Perhaps I'm not using the right
 keywords, but my literature searches haven't found the information I'm
 looking for.
 
 Thanks,
 -Dave
 
 -- 
 David Bapst
 Dept of Geophysical Sciences
 University of Chicago
 5734 S. Ellis
 Chicago, IL 60637
 http://home.uchicago.edu/~dwbapst/
 
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[R-sig-phylo] Reminder: Deadline for full talk abstracts for Conference on Informatics for Phylogenetics, Evolution, and Biodiversity (iEvoBio)

2011-03-23 Thread Hilmar Lapp

(Apologies if you receive multiple copies.)

This is a reminder that the deadline for submitting abstracts for full  
talks to the 2011 conference on Informatics for Phylogenetics,  
Evolution, and Biodiversity (iEvoBio) is Friday, March 25, 2011, which  
is just two days away. Please see http://bit.ly/iEvoBio2011_CfA for  
the full Call for Abstracts and author instructions.


As another reminder, full talks are only 1 of 5 kinds of contributed  
content that iEvoBio will feature. The other 4 are: 1) Lightning talks  
(5 mins long), 2) Challenge entries, 3) Software bazaar  
demonstrations, and 4) Birds-of-a-Feather gatherings. The Call for  
Challenge entries is also open (see http://ievobio.org/ 
challenge.html). Tentative submission deadlines are listed on the  
conference website (http://ievobio.org/program.html#dates).


More details about the program and guidelines for contributing content  
are available at http://ievobio.org.  You can also find continuous  
updates on the conference's Twitter feed at http://twitter.com/ 
iEvoBio, and there is an announcement-only Google group at http://groups.google.com/group/ievobio-announce 
. You can use the group's RSS feed (linked from its URL), or join the  
group to receive announcements by email.


iEvoBio is sponsored by the US National Evolutionary Synthesis Center  
(NESCent) in partnership with the Society for the Study of Ecolution  
(SSE) and the Society of Systematic Biologists (SSB). Additional  
support has been provided by the Encyclopedia of Life (EOL).


The iEvoBio 2011 Organizing Committee:
Rob Guralnick (University of Colorado at Boulder) (Co-chair)
Cynthia Parr (Encyclopedia of Life) (Co-chair)
Dawn Field (UK National Environmental Research Center)
Mark Holder (University of Kansas)
Hilmar Lapp (NESCent)
Rod Page (University of Glasgow)

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Re: [R-sig-phylo] weirdness in confidence intervals returned by ace, pic option

2011-03-23 Thread Emmanuel Paradis

Hi Nick,

With method = pic, the CIs are computed using the expected variances 
under the model, so they depend only on the tree. I've added a paragraph 
in the man page to explain this.


Cheers,

Emmanuel

Nick Matzke wrote on 22/03/2011 12:30:

Hi all,

This isn't crucial to my work at the moment since I am not using the PIC 
option of ace to do ancestral character estimation.  But while trying it 
out I noticed a very weird result that I can't explain...basically when 
I run ace on my raw trait values, I get the same sized confidence 
interval (97.5% CI minus 2.5% confidence interval) for all of my 
(drastically different) traits.


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