Re: [Biofuel] Amateurs are trying genetic engineering at home

2009-01-02 Thread Keith Addison
http://www.precaution.org/lib/09/prn_biohackers_unstitch_life.081227.htm

From: The Times (London, U.K.)
December 27, 2008

Biohackers attempt to unstitch the fabric of life

[Rachel's introduction: As we enter the new year, an astonishing new 
social phenomenon has emerged: amateur genetic engineers are working 
at home to improve various forms of life via genetic engineering. 
They call themselves biohackers and they acknowledge the danger of 
unleashing a genetically altered Frankenstein's monster on the 
public, but they argue that it was DIYers [do it yourselfers] who 
brought about America's other great technological revolution: that of 
the personal computer.]

By Chris Ayres in New York

At a loss for things to do this woozy post-Christmas weekend? Well, 
if you have access to a garage or basement -- or even just some extra 
room on your dining table -- you could always take up a hobby that is 
exploding in popularity across the Atlantic: genetic engineering. Or, 
to use the more fashionable term, biohacking.

Anecdotal evidence suggests that thousands of Americans now spend 
their free time consulting the internet, jerry-rigging laboratory 
equipment, and tinkering with the very foundations of life on Earth 
as we know it.

  Meredith Patterson is trying to rewire the DNA of yoghurt bacteria 
in her living room so that they will glow green to signal the 
presence of melamine

People can really work on projects for the good of humanity while 
learning about something they want to learn about in the process, 
says Meredith Patterson, 31, a computer programmer by day turned 
biohacker by night.

In her San Francisco dining room Ms Patterson is currently attempting 
to rewire the DNA of yoghurt bacteria so that they will glow green to 
signal the presence of melamine, the chemical that infamously turned 
Chinese-made baby milk formula into poison.

Ms Patterson says that she picked up the basics of genetic 
engineering from scientific papers and Google.

All she needed for her project was a jar of yoghurt, some jellyfish 
DNA -- purchased online for less than $100 (65 pounds sterling) from 
a biological supply company -- and a few pieces of lab equipment 
(including a DNA analyser), which she constructed herself for less 
than $25. Eventually, say experts, such equipment could be sold in 
kits: a kind of My Little Genetically-Altered Lifeform playset for 
adults.

While acknowledging the potential risk of unleashing a genetically 
altered Frankenstein's monster on the public, biohackers argue that 
it was DIYers [do it yourselfers] who brought about America's other 
great technological revolution: that of the personal computer.

Indeed, Apple and Google were created in hobbyists' garages, and have 
since gone on to change millions of lives for the better while 
contributing billions of dollars to the global economy.

Regardless, the growth in popularity of biohacking seems unstoppable. 
In Cambridge, Massachusetts, an organisation named DIYbio is busy 
setting up a community lab where people can use specialist equipment 
such as a freezer capable of storing bacteria at minus 62C.

The group's co-founder, Mackenzie Cowell, 24, who studied biology at 
university, predicts that some biohackers are likely to make 
breakthroughs in everything from vaccines to super-efficient fuels. 
Others will simply fool around, he says: for example, using squid 
genes to make tattoos glow in the dark.

All of which he believes will ultimately benefit humanity. We should 
try to make science more sexy and more fun and more like a game, he 
says.

Alas, not everyone agrees. Jim Thomas, of ETC Group, a biotechnology 
watchdog group, says that synthetic organisms could ultimately escape 
and cause outbreaks of incurable diseases or unpredictable 
environmental damage. Once you move to people working in their 
garage or other informal locations, there's no safety processes in 
place, he says, adding that terrorists could be inspired by amateur 
genetic tinkering to launch a devastating bioattack on America.

Mrs Patterson shrugs at such arguments, however. A terrorist doesn't 
need to go to the DIYbio community, she says. They can just enrol 
in their local college.

Copyright 2008 Times Newspapers Ltd.


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[Biofuel] Munich Re Highlights Climate Change Impact

2009-01-02 Thread Keith Addison
http://www.precaution.org/lib/09/prn_munich_re_on_2008.081231.htm

From: Financial Times (London, U.K.)
December 30, 2008

Munich Re Highlights Climate Change Impact

[Rachel's introduction: The re-insurance company Munich Re reports 
that weather-related catastrophes helped push losses to $200 billion 
in 2008, compared with $82 billion in 2007.]

By James Wilson in Frankfurt and Andrea Felsted in London

Financial damage and loss of life caused by natural disasters made 
2008 one of the most devastating years on record and showed the 
impact of climate change, one of the world's biggest reinsurers said 
yesterday.

Munich Re said weather-related catastrophes helped push losses to 
$200bn compared with $82bn in 2007.

Insured losses of $45bn were 50 per cent more than in the previous year.

This made 2008 the third most expensive year to the industry for 
catastrophe damage, continuing a long-term trend, the group said.

Climate change has already started and is very probably contributing 
to increasingly frequent weather extremes and ensuing natural 
catastrophes, said Torsten Jeworrek, a member of Munich Re's 
executive board.

Insurance companies are concerned by the impact of climate change on 
risk modelling and hence on financial performance.

Munich Re said the next UN climate summit, scheduled for late next 
year in Copenhagen, needed to quite clearly fix the route to halve 
output of greenhouse gases by 2050.

More than 220,000 people are estimated to have been killed by natural 
catastrophes during the year, including 135,000 in Burma during 
cyclone Nargis, where deforestation allowed a storm surge to reach 
further inland, said Munich Re.

Hurricane Ike was the year's most expensive event for insurers, with 
$15bn of insured losses.

The year was the fourth-worst hurricane season since reliable data 
have been compiled, while the US tornado season was unusually 
severe, Munich Re said. It was also the planet's 10th-warmest 
recorded year. All have occurred in the past 12 years.

The loss statistics for 2008 fit the pattern that the calculations 
of climate models lead us to expect, said Peter Hoppe, the 
reinsurer's head of geo-risks research.

Atmospheric warming meant the weather machine runs in top gear.

But the number of loss-producing events fell compared with 2007, 
Munich Re said.

The year's death toll included 70,000 in the earthquake that hit 
China's Sichuan province in May.

Munich's figures echo those of Swiss Re, which put insured losses 
from natural catastrophes at $43bn, of which Ike accounted for $20bn.

But Swiss Re estimates that there was another $7bn of insured losses 
from man-made disasters, taking total insured losses to over $50bn.

Copyright The Financial Times Limited 2008

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Re: [Biofuel] Amateurs are trying genetic engineering at home

2009-01-02 Thread Keith Addison
Hi Chip

Keith Addison wrote:
  Hi Peter

  Hi Keith ;

  I have made the point previously many times on-list that genetic
  engineering is not the answer to anything and in fact it will kill
  millions of people.  This is one way.

  Could be. I don't altogether agree with you though, I don't think
  genetic engineering should be written off. Certainly there's nothing
  good about the current offerings of GE crops, and plenty that's bad,
  and left in the hands of the current players it probably will kill
  millions of people, and indeed it already is. But the picture might
   be rather different if some real science were applied rather than
  just Monsanto's bottom-line, along with some sense and the
   precautionary principle. We don't need the crops anyway, we already
  have better crops, and there are better ways of developing them than
  GE. But it doesn't only apply to crops.f

Hey Keith:

I can't disagree with you here.
However, I just simply do not thing that our tools are anywhere near
where they need to be, before we even begin the engage in this
admittedly -really cool- technology.

I think you're referring mainly to GE crops? I'm not convinced that 
GE/GM has any useful role to play in crop development, though I 
wouldn't altogether rule it out. I'm sceptical that the current 
industry players are capable of producing anything useful, or even 
benign even if it's useless. Well, sod the industry, but there's more 
to the technology than the industry. And the technology is still in 
its infancy.

To develop in some sort of sane fashion, it might need this kind of 
open-source approach. Not to say we should just trust these folks 
(experts or not), but they sound rather more promising than do the 
likes of Monsanto. M$ vs Linux? Yes I know, that's not biology, but 
still. I'm not really defending it, but it might be a mistake to 
condemn it too hastily.

Anyway, it's not just crops, as I said, there are other applications 
where releasing the unknown on an unsuspecting biosphere might not be 
such an issue. There's a herd of goats in Canada that produce spider 
silk in their milk. The goats themselves seem quite happy, it's 
containable (unlike seeds), it seems to work, spider silk is useful 
stuff, there doesn't seem to be a plot to patent life or commandeer 
the commons.

Two areas where I wouldn't want to rule out the potential of GE are 
reclaiming the valuable materials we've been dumping in landfills all 
these years, and cleaning up the toxic legacy of the 20th Century, 
with millions of tons of pesticides dumped on the biosphere every 
year, along with about 100,000 chemicals nature's never encountered 
before. GE could turn out to be what it'll take. Hopefully some real 
science would be applied for a change.

The DIYbio story has raised quite a fuss, but I wonder how many of 
the objectors put the same energy into objecting to Monsanto, and if 
not, why not.

All best

Keith


Cautionary Principal doesn't get close. I think we need modeling
technologies many orders of magnitude beyond where we are now before
we can reliably predicted with any confidence at all, where gm
experiements will be in as many generations as it takes to have some
clue that the last state is in fact, better than the beginning state.

For instance, *if* we had climate modeling that could predict weather
with good confidence years in advance, then we might be able to consider
what adding frog dna to vegetables might do in 10, 20, or 50 generations
of the product itself, not to mention the stuff done to the dna of
the critters that eat it, pass it into the waste stream, and so on.

And we ain't there, not even nearly there, with all the computing
power we can muster, we can barely touch next week.

I think the term is 'sensitive dependence on initial conditions'.
pop culture likes to oversimply this as 'the butterfly effect' and
completely miss the point. But you know what I mean.

This, if it were possible to de-couple the technology from the takings
of the commons of life itself, known as the bio-patent business, would
be challenge enough to keep the best and brightest working hard for
a while yet, , , working on models, not real living stuff that gets
released into the biosphere.

And since it isn't being decoupled from the enclosing of the commons
of life, into corporation ownership of life itself, I find it
particularly reprehensible.

but that's just me.

  So many times with technology we find that the last condition is
  worse than the first.  Extrapolating this out to its logical
  conclusion, we find that all technology advances are bad.

  :-) A little too sweeping Peter (useful things, brooms).

  Could this be the reason that almost all religious leaders (and by
  that I mean Jesus, Mohammad, Bhuddha, etc) shun technology.
  
  Do they? Jesus was a carpenter, what did he use to cut wood, his
  teeth? He said nice things about chickens, but chickens are not as
  Mother Nature made them, they're a 

Re: [Biofuel] Amateurs are trying genetic engineering at home

2009-01-02 Thread Keith Addison
Hi Peter

snip

Many thanks to moderator Keith, we are WAY off the thread.

You're welcome. :-)

Definitely off-thread yes, not just the topic itself, but also the 
a-priori mode of reasoning, which may be a valid basis for discussion 
of Christian theology but not for an assessment of technology, IMHO.

All best

Keith



BR
Peter G.
Thailand
www.gac-seeds.com


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Re: [Biofuel] Jatropha and ethanol

2009-01-02 Thread Keith Addison
Hello David

Dear list experts,

I note a good deal of information in the list about using Jatropha (J.
curcas) for biodiesel, and, mindful of repeated admonitions, I've looked
for information about the questions I have in the archives, but I've not
yet seen answers directly to my questions. My own background is in
biogas, and I have only recently started learning about biodiesel and
ethanol, so I'm an admixture of knowing and novice. May I ask a few
questions?

But of course.

(And Kieth, no doubt there are many gems in the archives
which of which you know, yet which I missed. Please feel free to educate
me regarding their nature and location.)

I keep finding surprises there. There are 74,000 messages, 498.4 Mb 
of it, and that's in a compressed format, it's at least 500 books' 
worth.

I've been contacted about a project in south Asia which would involve
planting 600 ha to Jatropha, to produce 3,500 tonnes of biodiesel
annually. (Based on what I've seen about Jatropha, that may be
optimistic for yield, but I'm just presenting the information as given
to me.)

Obviously then that also means either the use of a good deal of methanol
(as presently planned), or (as I have suggested), producing either
ethanol or butanol through fermentation and using one or more than one
together for separation. The oil cake resulting from oil extraction
would be feedstock for a biogas plant. The biodiesel plant is presently
being considered as a prototype for a number of such plants, and among
the key goals of the project are social and economic development, not
merely the production of fuel, and although the project expects a
profit, my impression is that things would be operated to produce a
balance of outcomes.

I quite often hear of projects that sound similar (they often want 
advice from us).

I'm always suspicious of the best crop or the best technology 
approach (see eg 
http://journeytoforever.org/fyi_previous4.html#1511Technology and 
the poor), and 600-ha monocrop plantations don't have a very good 
record.

Why jatropha? Whose choice was it? On what grounds? Because there's a 
tax rebate for it in India? (There is still, isn't there?) Because 
it's hyped such a lot? I'm not being too sceptical, those are common 
reasons.

If social and economic development are truly key goals, then the 
approach has to be bottom-up, not top-down. How are the local people 
to benefit? Did anybody ask them yet?

There are other choices besides jatropha. The hype says the jatropha 
seedcake makes a great organic fertiliser, but the truth is that a 
non-poisonous seedcake that can be fed to livestock is generally much 
more useful - in fact it can make the difference to whether a project 
is feasible or not. Generating biogas from the cake first might make 
better numbers, but, again, how would the local community see it?

What oil crops do the local farmers use, or know of? Wouldn't using 
mixed species something like J. Russell Smith's Tree Crops 
http://journeytoforever.org/farm_library.html#treecrops but with a 
bias towards oil production (easily done) be better? Such an 
agroforestry project, with intercropping, livestock grazing and so 
on, would seem to offer much more than a monocrop plantation could, 
and be more likely to be adaptible to local conditions, and the local 
community.

Amid all the jatropha hype, this report is interesting, I don't know 
if you saw it, from GRAIN:
Jatropha - the agrofuel of the poor? GRAIN July 2007
http://www.grain.org/seedling/?id=480

That whole July 2007 issue of Seedling is worth a look:
http://www.grain.org/seedling/?type=68

So, first question: Although I've reviewed the project overview, which
mentions that the biodiesel mixer will be batch loaded, as yet I have no
information about the size of the unit. What size would/should it be to
produce that much biodiesel annually?

You could probably set the upper limit of what would qualify as DIY 
or homebrew or local coop or Appropriate Technology-level biodiesel 
production at about 1,000 gallons a day, which is about a tenth of 
what they're planning. They'll be wanting an industrial processor. If 
the biodiesel plant is to be a prototype for a number of such plants, 
they'll also be industrial processors.

Second, am I near the mark with suggesting that the project consider
producing ethanol (or butanol) rather than purchasing methanol?
Certainly it will provide increased challenges to use ethanol, and
perhaps even more to use butanol (in either case including adding
complexity to the process), but I would think for a plant this large,
with good access to land (albeit perhaps marginal land) and given the
low labor costs in the area, it may make sense, although one problem may
be training personnel. Yes? No?

Nobody does it. A few homebrewers use ethanol, and they seem to be 
the only ones, virtually all or all commercially produced biodiesel 
is methyl esters, not ethyl esters. The ethanol biodiesel process is 
not easy, but the main 

Re: [Biofuel] Jatropha and ethanol

2009-01-02 Thread Keith Addison
Hello again David

Re this:

You could probably set the upper limit of what would qualify as DIY
or homebrew or local coop or Appropriate Technology-level biodiesel
production at about 1,000 gallons a day, which is about a tenth of
what they're planning. They'll be wanting an industrial processor. If
the biodiesel plant is to be a prototype for a number of such plants,
they'll also be industrial processors.

Sorry, I got the numbers wrong, took gallons for litres - it's about 
a third of the planned production, not a tenth.

Best

Keith



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Re: [Biofuel] Jatropha and ethanol

2009-01-02 Thread David House
.

Although sodium soaps are soluble (they dissolve in water), the
calcium and magnesium soaps are much less soluble, and so they
precipitate (they come out of solution) and they form an important
component of scum. Scum, the portion of the slurry which floats on
the liquid portion, forms a dense hard mat if left alone, which will
eventually completely stop gas production in a biogas generator
These insoluble soaps, and various greases and oils, bind the other
materials in the scum together and make it more difficult to break
up the scum. In a similar way, wet hair, caught on a screen or in a
trap in a bathtub drain, gathers oils, greases, and soaps, and
begins to stick together in a way that clean wet hair would not.
TCBH, p. 63


Even so, the presence of so-called soaps is essentially inevitable. 
(Although fatty acids, in the presence of Ca++ ions, or Mg++ ions, form 
insoluble soaps, these acids are nevertheless extremely important in 
biogas production. Even if we did not want them to be present in the 
slurry, they would still be formed in the process of disassembling more 
complex molecules, for the volatile acids passed on from the AF 
[acid-forming] bacteria to the MF [methane-forming] bacteria are mostly 
fatty acids. ibid)


 I know that depending on how one handles this waste stream, it can be burned 
 (at high temp), composted, used in soap-making, used to supplement the oil 
 cake for biogas production, used in Clostridium fermentation to produce ABE, 
 used to dry ethanol (and butanol?), et al. Are there other options? Among 
 those possibilities, which might best serve the mix of goals?
 

 It depends whether you're talking of the raw by-product or the separated 
 components. Did you read the Glycerine page at the Journey 
 to Forever website? 

I did. That was part of the reason why I said depending on how one 
handles... it..., although my sentence could have been clearer. The 
project information I have says that they intend to use a suitable 
packed column, condenser, and receiver... to recover excess amount of 
Methanol in the system. As yet that means very little to me. I'm not 
sure, for example, whether they would take only the settled fraction of 
the trans-esterified result (what's the term of art for this?) and put 
it through such an extraction process, or whether they would put the 
whole lot through it, given that a minor portion of the excess methanol 
is mixed into the biodiesel fraction (if I understand correctly). 
Likewise, I don't know whether such a process would assist in 
separating-- or if one would need to separate-- further components of 
the glycerol-containing fraction, following such a process. The project 
information says they intend to make soap from the glycerol.

One can make biogas from methanol, and it therefore seems possible to me 
based on what little I know about its contents that the whole 
unseparated glycerol fraction of the trans-esterified result could 
likely be put in the digester. However, given the value of methanol 
relative to biogas, that does not seem like an entirely sensible option.



 Lastly, the information I have says that Furthermore, the process to 
 manufacture biodiesel... has no waste at all [excepting the oil cake and 
 glycerol]. The process employed has no emissions and absolutely no effluent 
 treatment. I don't see how that can be correct. Can that be the case?
 

 Did you add [excepting the oil cake and glycerol]? 

I did, intending not necessarily to indicate they were waste products, 
but rather that there were such by-products.


 The process itself has no emissions, but the power supply used might have 
 emissions. Would that be the biogas plant? There's also the water used for 
 washing, but it can all be accounted for with no addition to the waste 
 stream. I think that's all detailed at the Biodiesel section of the Journey 
 to Forever 
 website.
   

Again I know very little. It sounds as though the organization which is 
hiring me-- which itself is secondary to the project-- is planning on 
selling the biogas, or at least some of it.

Still, it surprises me that given the use of so many chemicals (such as 
methanol, which evaporates, given half a chance), that there would be no 
emissions. In any case, as the bacteria of the world prove every day, 
one being's waste is another being's meal ticket.




d.
-- 
David William House
The Complete Biogas Handbook |www.completebiogas.com|

Make no search for water.   But find thirst,
And water from the very ground will burst.
(Rumi, a Persian mystic poet, quoted in /Delight of Hearts/, p. 77)
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