Re: [aroma.affymetrix] Encountered ERROR while extracting chip effects

2014-12-15 Thread Henrik Bengtsson
On Thu, Dec 11, 2014 at 4:52 AM, Rishi Das Roy rishi.das...@gmail.com wrote: Hi Thanks for your reply. I can understand that I can identify alternative splicing through FIRMA more confidently. However I also want to identify differentially expressed genes. I have not find any documentation

[aroma.affymetrix] Encountered ERROR while extracting chip effects

2014-12-10 Thread Rishi Das Roy
Hi, I was trying to perform RMA on *Mouse Exon 1.0 ST Array* as described in aroma website (link http://www.aroma-project.org/replication/RMA) using custom cdf MoEx-1_0-st-v1,coreR1,A20080718,MR.cdf.gz

Re: [aroma.affymetrix] Encountered ERROR while extracting chip effects

2014-12-10 Thread Henrik Bengtsson
Hi, you cannot use RMA on exon-arrays, you will need to use exon-aware method such as FIRMA (e.g. http://aroma-project.org/vignettes/FIRMA-HumanExonArrayAnalysis). Hope this helps Henrik PS. For http://aroma-project.org/replication/RMA, I've updated it to use `theta - extractTheta(ces)`