Re: [ccp4bb] Solvent content of membrane protein crystals

2007-09-25 Thread Edward Berry
Das, Debanu wrote: Hi, There are at least 4 methods to try to estimate amount of detergent in a membrane protein crystal . In summary, someone wanting to estimate amount of detergent in their crystals and have sufficiently large and numerous crystals, could try out any of

Re: [ccp4bb] mosflm orientation matrix and symmetry

2007-09-25 Thread Nicholas K Sauter
Bryan, I think it would be more correct to say that the orientation matrix file applies to the Bravais type (e.g., hP--hexagonal primitive). Within the Bravais type you can substitute a different space group prior to integration. Nick From: Bryan W. Lepore [EMAIL PROTECTED] does the

[ccp4bb] Quick soak method

2007-09-25 Thread Derek Logan
Hi, I'd like to find out how successful the quick soak method for heavy atom derivatisation proposed by Radaev and Sun: Sun PD, Radaev S, Kattah M. Generating isomorphous heavy-atom derivatives by a quick-soak method. Part I: test cases. Acta Cryst. 2002. D58:1092-1098. has been in

Re: [ccp4bb] mosflm orientation matrix and symmetry

2007-09-25 Thread Winter, G (Graeme)
Hi Bryan, So, there are two answers to this question. If you are changing the spacegroup within the same lattice type (e.g. tP) then it is fine (this is probably what you mean by crystal system) - though you can do this by just running reindex symm whatever afterwards. If you want to change from

[ccp4bb] Crystallographic Post-doctoral Position in Champaign-Urbana, IL

2007-09-25 Thread Satish Nair
An NIH funded post-doctoral position is available to work as part of a multi-disciplinary effort on structure determination of proteins involved in antibiotic biosynthesis (Science 311, 1464-1467). Candidate should have a background in standard protein purification, synchrotron data

[ccp4bb] SRS AP50 (April - December 2008) Open for Applications

2007-09-25 Thread Ellis, MJ (Mark)
Dear SRS Users, Applications are now invited for beam-time in allocation period 50 (April - December 2008) on PX station 10.1 at the SRS. The deadline for submission of proposals is Thursday 1st November. Further details on how to apply for beamtime can be found here :-

[ccp4bb] Pymol installation

2007-09-25 Thread joao
Dear all. We have had success installing Pymol on any 32 bits PC, but we are having some troubles with 64 bits. The OS is linux ubuntu kernel: 2.6.15-29-amd64-generic x86_64 The pymol version is: pymol-0_99rc6-bin-linux-x86-glibc23.tgz The error message is: freeglut (./pymol.exe): ERROR:

[ccp4bb] Alignment question

2007-09-25 Thread Ibrahim M. Moustafa
Hi all, I'm looking for a webserver that can take the alignment and produce a nice figure with the alignment annotated with sec. structure on top, and draw boxes around conserved residues. Long time ago I had used a server doing exactly what I described; unfortunately, I lost the

Re: [ccp4bb] Alignment question

2007-09-25 Thread Ethan Merritt
On Tuesday 25 September 2007 14:22, Ibrahim M. Moustafa wrote: I'm looking for a webserver that can take the alignment and produce a nice figure with the alignment annotated with sec. structure on top, I use TeXShade http://www.ctan.org/tex-archive/help/Catalogue/entries/texshade.html

Re: [ccp4bb] Alignment question

2007-09-25 Thread Ibrahim M. Moustafa
Thanks a lot for Francesco, Ethan, and Jessica. Yes, it is ESPrit. For unknown reasons, when I use keywords: sequence + alignment + tools in google that server never showed up. It is also worthy to look at the other servers suggested in the reply given below: Francesco Charlie Bond has a

Re: [ccp4bb] Quick soak method

2007-09-25 Thread artem
Hi, I do not use their method as such - however, I love heavy atom soaks and do them any time I can, so I've got very similar experiences in the past. Heavy atoms can bind very quickly even from quite dilute solutions - the quickest I've ever soaked (and got useful data) was sodium

Re: [ccp4bb] Quick soak method

2007-09-25 Thread James Whisstock
Hi - sorry - rather than iodine I meant to say we had had success with Potassium Iodide (1M for 20 seconds)! Cheers James [EMAIL PROTECTED] wrote: Hi, I do not use their method as such - however, I love heavy atom soaks and do them any time I can, so I've got very similar experiences in

Re: [ccp4bb] Quick soak method

2007-09-25 Thread Jacob Keller
I also solved a structure on our home CuKa source by SIRAS with 20-60sec in 0.5-1M KI. JPK ==Original message text=== On Tue, 25 Sep 2007 6:55:28 pm CDT James Whisstock wrote: Hi - sorry - rather than iodine I meant to say we had had success with Potassium Iodide (1M