Hi
This is a problem that occasionally cropped up if there were large gaps in the
alignment between target and model. It has been fixed in the ccp4-6.0.99d test
release. You can either download and install the test release, or if you let me
know what operating system you are using, I will
Dear Bill,
A person started his career as a Laboratory Technician and raised to a
level of Associate Professor(and still dont know the next highest level) ,
this transitions in Sanskrit called as avatars (every state serve its
purpose of its existence) ,
there is always good reasons for an
Hi Folks,
A post-doctoral position is available in the labs of Drs. Jay F.
Storz and Hideaki Moriyama at the University of Nebraska. The NIH-
funded research project involves an experimental investigation of
molecular adaptation. Specifically, the goal is to identify
mechanisms of
Thank you for all your advices.
I usually employ salt gradient in MonoQ for my proteins, but I just wonder
if we can use pH gradient in the same column and see if it can improve the
separation.
I know the condition that my protein binds to Mono Q, so I will try to
titrate toward its pI to reduce
John,
You can adjust your ionic strength not only with NaCl/KCl/etc but also
by increasing the concentration of the components
in your buffer mixture. If you do it right, I can assure you can get a
linear pH gradient.
And, as mentioned earlier by Tom, you can use both pH and salt gradients
to
Hi Nadir,
No I didn't and I am not concerning CHROMATOFOCUSING, and you are right, I
have been thinking of using both pH and salt gradients to refine the
separation. Thanks!
Matt
2008/6/25 Nadir T. Mrabet [EMAIL PROTECTED]:
John,
You can adjust your ionic strength not only with
Hi there,
I suspect my reducing agent TCEP-HCl is bound to my protein in the
crystal. I am trying to put a TCEP molecule into the bulky density,
but could not find any available coordinates of TCEP.
Do you know any where I can download the TCEP coordinates or any where
I can build one starting
Hi,
if a compound is not in the hic-up data base of the USF
(http://xray.bmc.uu.se/hicup/)
I usually go to NCBI Pubchem database (TCEP e.g. at
http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=119411loc=ec_rcs)
, download the sdf file, feed it into a programm that can read it and
put
Thank you very much.
My operating system is Fedora5( I don't know whether this information is
enough).
Stein, ND (Norman) [EMAIL PROTECTED] 写道： Hi
This is a problem that occasionally cropped up if there were large gaps in
the alignment between target and model. It has been fixed in
