Dear all,
Recently we have collected one set of data which is processed to 2.9A and
3.0A and the Wilson-B values are 50.1 and 59.1, respectively. As for the
completeness of the highest shell is only 66% in 2.9A (80% in 3.0A), we use
the dataset with 3.0A for phasing and refinement.
Everything is
Robert Esnouf wrote:
Dear All,
I see that the UK is catching up and the NVIDIA Geforce 3D Vision Bundle (Samsung
2233RZ 22 LCD + NVIDIA GeForce 3D Vision Glasses) is now available for ~£350.
Can I reveal my ignorance and ask whether (with a suitable graphics card) this kit
gives decent 3D
Dear Patrick
Long ago we (Cheetham et a (1992) J Molec Biol 224:613-628) did some
refinements on hen lysozyme + substrate complexes at 1.75A and 2A
resolution and showed that B and occupancy are negatively correlated,
especially at 2.0A. In simplistic terms this is because at medium
Hi
I am recruiting for a postdoc position in my group, available
immediately. This is the Protein Crystallography group of the
Structural Genomics Consortium, Oxford. In particular, hard-core
crystallographers strong on theory and (optionally) with a bent for
programming, are invited to
Hi Pat
I concur with George, we routinely refine together the group B factor
and occupancy of our ligands and frequently see significant deviations
of the group occupancy from the starting value even if it is highly
correlated with the group B factor (the significance test takes account
of the
Stuart, Robert, Others,
I believe Stuart is confusing the Samsumg 2233rz frame-sequential stereo 3D
display with the Zalman M220W interlaced stereo 3D solution.
Due to lack of driver support, as far as I know, the Samsung isn't yet
supported by any OpenGL-based software, although nVidia has
Dear all,
Recently we have collected one set of data which is processed to 2.9A and
3.0A and the Wilson-B values are 50.1 and 59.1, respectively. As for the
completeness of the highest shell is only 66% in 2.9A (80% in 3.0A), we use
the dataset with 3.0A for phasing and refinement.
Everything is
Warren DeLano wrote:
Stuart, Robert, Others,
I believe Stuart is confusing the Samsumg 2233rz frame-sequential stereo 3D display with the Zalman M220W interlaced stereo 3D solution.
Due to lack of driver support, as far as I know, the Samsung isn't yet supported by any OpenGL-based
Dear All,
I would appreciate if anyone could recommend any company for long peptide
synthesis? It will be better if it is reliable and reasonable priced.
The peptide we want to synthesis is around 40-50aa long, should be HPLC
grade and above 98% purity.
Thank you!
Ping Sun
National Cancer
Use the FRET channel - it's ideal for SYPRO orange.
Artem
Sorry Charlie for the late reply.
I'm using the CFX96 5 channel with Sypro and other dyes. I tested the
machine against the Eppendorf product nd decided to go for the Biorad
version as running identical samples gave better results on
ping,
we had great success as far as price and level of synthesis
with a company called Synbiosci, Livermore, CA.
I am no way connected to this company but just a suggestion.
hope it helps
psp
On Mon, Jun 1, 2009 at 10:31 AM, ping sun ccp4@gmail.com wrote:
Dear All,
I would appreciate if
Hi,
I have to take issue with one of Ian's points. His statement that
the integral of the electron density does not change with the change
in a B factor but does change with a change in occupancy is the equivalent
to stating that the integral of an occupancy difference map feature is
nonzero
Dear Jain,
The Wilson plot is based on the assumption that you can model your
structure with a collection of atoms randomly distributed in the unit
cell. This model is very poor at low resolution, say, 4.5A and lower.
Since the Wilson B depends on the change in scattering as a function of
Hi,
I've used the CFX96 with the FRET channel for DSF and found it to work well.
Gary
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
ar...@xtals.org
Sent: Monday, June 01, 2009 11:47 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] BioRad
A PhD and a Postdoctoral position are immediately available
in the Ribosome Crystallography Group led by Prof. Dr. Paola Fucini,
in the Cluster of Excellence for Macromolecular Complexes and Johann
Wolfgang Goethe University.
The candidates will participate in the ongoing research of the
Dear HengChiat Tai
(a) According to me Seed Solutions should be stored depending on the
stability of the crystals in the drop. If your crystal goes bad quite early
then you have to make fresh seed frequently. Its always a good habit to make
fresh seed, everytime you do streaking.
(b) The
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