I think the origin of this thread is that the original Zalman Monitors are no
longer sold and Zalman only announces these new ones now.
We are waiting for their availability (in Germany) and we have no doubt that
they are going to work like the previous models did - the technology (which is
about
Is there anyone want to participate in a Project Evaluation, free of
transformation fee but no payment. Held in Middle August in Le-Shan, China. You
can see the LeShan Grand Buddha and E-Mei Mountain by the way, which is one
of the best scenic areas in China.
The topic of evaluation is
Is there anyone want to participate in a Project Evaluation, free of
transformation fee but no payment. Held in Middle August in Le-Shan, China. You
can see the LeShan Grand Buddha and E-Mei Mountain by the way, which is one
of the best scenic areas in China.
The topic of evaluation
While what Ed is suggesting has worked for me too and, and as far as know, is
probably the only way to get mixed iso/aniso B-refinement to do what one
intends to do, perhaps Garib et al. can introduce a more elegant way of
specifying in the GUI which residues range should be refined with
Dear colleagues,
this is to remind you that the Murnau Conference 2010 on Structural Biology of
the Modern RNA World will be taking place from 13 to 16 October, 2010.
As you might already know, the Murnau Conference is an international meeting
that covers medically relevant and timely topics
In the realm of nVidia 3D vision technology, as opposed to Zalman:
ASUS showed a 27 monitor at CeBIT, the PG276H, which is not yet
available (Some articles say June 2010, some say Q4 2010). The pixel
resolution, 1929x1080, will be the same as the current crop of 23
monitors (e.g. Acer
Hi，
I am using CNS to refine the structure.But when I come to bgroup refinement,
the
produced bgroup.pdb have many residues with very high B factor. I read the
bgroup.inp, and found the item‘B-factor groups'with following description:
{* select atoms in group 1 - protein mainchain example *}
The problem is the unrestrained nature of the grouped b-factor
refinement itself. Read this thread
http://www.mail-archive.com/ccp4bb@jiscmail.ac.uk/msg14133.html
In a nutshell, just stick with (properly wighted) individual B-factors.
On Fri, 2010-07-16 at 10:57 -0400, hongjunyu wrote:
Hi，
I
Hi Hui,
I think most of us can't do much with rar archive files.
This is a Windows thing I believe and my Linux system tells me
Archive type not supported...
Fred.
hui yang wrote:
Hi all,
I just collected a data set from a long-spindle-shaped crystal. The
data has been scaled to P1
Hi Fred
You probably need to install package 'unrar-free', e.g. see:
http://packages.ubuntu.com/hardy/i386/unrar-free/download .
Cheers
-- Ian
On Fri, Jul 16, 2010 at 3:37 PM, Vellieux Frederic frederic.velli...@ibs.fr
wrote:
Hi Hui,
I think most of us can't do much with rar archive
rar and unrar are available for linux. Something similar to aptitude install
unrar or zypper -i unrar, depending on the distribution, might even be faster
than repacking the archive.
Tim
On Fri, Jul 16, 2010 at 04:37:04PM +0200, Vellieux Frederic wrote:
Hi Hui,
I think most of us can't do much
The Varani group at the University of Washington is looking for a postdoc to
work in the area of RNA structural biology, primarily on RNA:protein and
protein:protein complexes involved in RNA processing.
Candidate must have a PhD in biochemistry or related fields, and a background
in
Dear Hui,
I am not sure what you mean by the diffraction patterns looking all the same.
They don't look the same to me, although they surely don't display any lunes.
The diffraction goes quite a bit beyond 3.5A, but I don't know HKL2000 well
enough to explain why it refuses to integration
Pointless thinks it's P42 21 2 with a reasonably high confidence (though that
doesn't exclude twinning)
Phil
data.log
Description: Binary data
On 16 Jul 2010, at 17:30, Tim Gruene wrote:
Dear Hui,
I am not sure what you mean by the diffraction patterns looking all the same.
They don't
Dear CCP4BB-ers,
I should have realised that if you ask CCP4BB for feedback, feedback is what
you will get. In spades :-)
Some feedback to your (public and private) feedback:
- colour-blindness - we have switched from dark EMBL green to light EMBL green
to accomodate all kinds of
Dear Hui,
About the so-called weird pattern, it is not a surprise at all!
You mentioned you had a long-spindle shaped crystal with large cell
dimensions (208 A) and a very possible P4x2x2 SG (proved by Phil). It
is very likely the longest dimension of the crystal (parallel to cell
edge
Sorry, I think my reply was not case in the attached images! I
happened to have the problem to see the image but I could now see all
of them. I think Tim was correct, the patterns are not the same at
all. Lijun
On Jul 16, 2010, at 12:23 PM, Lijun Liu wrote:
Dear Hui,
About the
Hi everybody,
I just calculated a self rotation function from the data used for molecular
replacement (what by the way did not worked) and saw no peak at all. Does this
not mean that there is only one molecule in the AU and no additional
crystallographic axis? Matthews suggested 3-7 molecules.
All:
We are running CCP4 v6.1.3. I have recently tried to use the Program SC, which
calculates Surface Complementarity of an interface between two components. I
can get it to run fine, but I have one complex, where one of the partners has
carbohydrate residues, which have atoms that are not
Pure translation NCS?
On Fri, 2010-07-16 at 20:04 +, Marie Lacroix wrote:
Hi everybody,
I just calculated a self rotation function from the data used for
molecular replacement (what by the way did not worked) and saw no peak
at all. Does this not mean that there is only one molecule in
Hi Steven
SCRADII is defined in $CINCL/defaults.def which if I recall correctly
implies that by default it will look in $CLIBD for the file, unless you
specify a directory explicitly.
So you could try:
% sc XYZIN foo.pdb SCRADII ./my_radii.lib
Hope it works!
Cheers
-- Ian
On Fri, Jul 16,
Ian:
Thank you for responding at 22:00 on a Friday evening.
Your suggestion of using ./my_radii.lib led to the program recognizing the file.
CCP4 should considering modifying the sc.doc document to make the fact that the
directory needs to be specified explicit.
Steven
From: Ian Tickle
Hi All,
I used JLigand to form a covalent bond between ligand, Benzoate and residue Ser
and followed the tutorial in the JLigand website and refined it. How shall I
know that a covalent bond is existing now? It does not show in coot as a bond.
Also although i had inserted the line with Link
Hi CCP4ers:
Recently I am trying to fix a helice, but found that it is so hard for
me because of the poor resolution(3.2A) and also a unvisible loop near it,
so is there any way to just fix a helice ? can I get it out of the whole
structure to refine it?
Best regards
--
Xinghua Qin
College
Dear Fred. and Martyn:
Thank you for your replies. I am new to CNS and I just followed the CNS
website protocols. I got your ideas. Since my resolution is 2A, I should skip
the bgroup refinement.
Hongjun Yu
Dear CCP4BB users:
I have recently switched to use REFMAC5 instead of CNS in the final
refinement of a structure. The TLS method gave much better R/Rfree
values than did CNS (17/22 vs 25/28 at 2A).
In CNS there is a harmonic term to tether some specified atoms to
their original positions. I use
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