Re: [ccp4bb] Refmac- solvent mask

2012-09-27 Thread Kiran Kulkarni
Thank you very much James ! -kiran From: James Holton jmhol...@lbl.gov To: CCP4BB@JISCMAIL.AC.UK Sent: Wednesday, September 26, 2012 9:58 PM Subject: Re: [ccp4bb] Refmac- solvent mask Yes, it's called a partial structure.  You input the mask as structure

[ccp4bb] Position available: Postdoctoral fellow in New York

2012-09-27 Thread Karim-Jean Armache
*Postdoctoral Fellow: Structural biology of chromatin modifiers.* A postdoctoral position is available in the laboratory of Dr. Karim-Jean Armache, Assistant Professor in the Structural Biology Program at the Skirball Institute of Biomedical Research—NYU Medical Center, New York, USA.We are

[ccp4bb] Poor baculovirus stability at 4C?

2012-09-27 Thread aaleshin
Sorry for an off-topic question. We began experiencing a sudden reduction in stability of baculovirus stock stored at 4C, like the titer drops more than 5 fold in 3-5 month. The only difference compared with previous preparations is a switch from Gibco SF900-II to a media from Lonza

Re: [ccp4bb] Poor baculovirus stability at 4C?

2012-09-27 Thread Dima Klenchin
We began experiencing a sudden reduction in stability of baculovirus stock stored at 4C, like the titer drops more than 5 fold in 3-5 month. The only difference compared with previous preparations is a switch from Gibco SF900-II to a media from Lonza (Insect-XPRESS). Could it be due to the

Re: [ccp4bb] Poor baculovirus stability at 4C?

2012-09-27 Thread Vitali Stanevich
Alexander, We produce baculovirus at Gibco SF900-II + 10% heated FBS + 1xPSG and then store at 4C routinely. And it seems to be quite stable. We have a virus that had no change for more than 2 years: we judge by the volume of it needed to infect_cells/produce_protein, no titer measurements. We