[ccp4bb] streaming of the 2013 CCP3 Study Weekend

2013-01-02 Thread Charles Ballard
Dear All it is the time of year again when we will be (attempting) to stream the Study Weekend live from Nottingham on the 4th and 5th of January. This time round we have a different method for you to access the stream The link for watching the Live Event is: 87.83.31.218/stfc Open

[ccp4bb] postdoctoral position at the NIH on microtubule regulators

2013-01-02 Thread ANTONINA ROLL-MECAK
A postdoctoral position is available in the Cell Biology and Biophysics Unit headed by Dr. Antonina Roll-Mecak at the National Institutes of Health. The Roll-Mecak Laboratory is interested in understanding the interplay between microtubules and their regulators and how tubulin

[ccp4bb] Expert opinion for optimizing ethylene glycol crystallization condition

2013-01-02 Thread Sankaranarayanan Srinivasan
Dear all, A very happy new year to all. I would appreciate some expert advice on optimizing a crystallization condition in which the initial hits were obtained with ethylene glycol as the main precipitant. Here is the summary of things tried. We have a protein, size (31Kda) and the starting

Re: [ccp4bb] Expert opinion for optimizing ethylene glycol crystallization condition

2013-01-02 Thread Enrico Stura
Dear Sankaranarayanan Srinivasan Try replacing both glycerol and ethylene glycol by propylene glycol in part or completely. Enrico. On Wed, 02 Jan 2013 18:59:21 +0100, Sankaranarayanan Srinivasan texs...@gmail.com wrote: Dear all, A very happy new year to all. I would appreciate some

Re: [ccp4bb] Expert opinion for optimizing ethylene glycol crystallization condition

2013-01-02 Thread David Briggs
Hi Shankar, I don't claim to be an expert, but I've used ethylene glycol as a cryoprotectant many times, and 30% (v/v) would probably be sufficient for proper freezing without any further addition. Did you try and shoot a crystal at room temperature? Hth, Dave On Jan 2, 2013 5:59 PM,

[ccp4bb] domain predication

2013-01-02 Thread LISA
Dear All, I have a big protein having more than 2000 amino acids. which sever is good to predicate the domains or motifs it has. Thank you. lisa