[ccp4bb] postdoc position available in antibiotics uptake, Newcastle University (UK)

2013-03-18 Thread Bert Van-Den-Berg
An immediate position is available for up to 5 years for an enthusiastic and highly motivated postdoctoral Research Associate in the area of Outer Membrane Uptake of Antibiotics. You will participate in a European consortium (TRANSLOCATION) consisting of academic labs, small biotech firms and

Re: [ccp4bb] Build DNA to fit density

2013-03-18 Thread Nicolas Foos
Hi Wei, If you have an interpretable density, the efficientest way in my opinion, is to construct manually (only 6bp). You can use coot and the button add residue. Coot can add nucleotide directly at the extremity of your existing nucleic acid model. Hope to help you. Nicolas Le 18/03/13

Re: [ccp4bb] space group determination problem

2013-03-18 Thread gengxiang zhao
Dear All, Firstly, thanks Ian and Michael's efforts on this issue and everyone's. We pointed the space group to P43212, then it works okay. Best Wishes, Gengxiang On Fri, Mar 15, 2013 at 1:26 PM, Ian Tickle ianj...@gmail.com wrote: Michael, yes sorry I had (temporarily) forgotten about

[ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

2013-03-18 Thread Jacob Keller
Dear List, Does anyone know of a source of quantification of membrane proteins in garden-variety eukaryotic cell lines, e.g. HEK or HeLa cells (incidentally, it just occurred to me that probably all HeLa cells are XX--seems right, no?) I am looking for the highest-expressed, particularly, and

[ccp4bb] international PhD program at IGBMC, deadline for application 22.3.2013

2013-03-18 Thread Bruno KLAHOLZ
Dear all, kind reminder for the International PhD program at the IGBMC, notably for integrated structural biology, with a deadline for applications 22nd of march 2013: http://phdprogramme.igbmc.fr/ see also http://www.igbmc.fr/research/department/3/ Best regards, Bruno Klaholz

Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

2013-03-18 Thread Edward A. Berry
In the mitocondrial inner membrane (which is very protein-dense) I believe the most abundant protein is the adenine nucleotide transporter. (e.g. pdb1OKC). Single chain or homodimer, but apparently its not very easy to crystallize. Jacob Keller wrote: Dear List, Does anyone know of a source

Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

2013-03-18 Thread Jacob Keller
I don't want to crystallize the protein--I have another reason Jacob On Mon, Mar 18, 2013 at 1:18 PM, Edward A. Berry ber...@upstate.edu wrote: In the mitocondrial inner membrane (which is very protein-dense) I believe the most abundant protein is the adenine nucleotide transporter. (e.g.

[ccp4bb] Balbes serv

2013-03-18 Thread Ville Uski
Dear Crystallographers, the Balbes webserver is now hosted at the Research Complex at Harwell, on the Rutherford site. The new URL is http://rcoisin.rc-harwell.ac.uk/BALBESSERV/ The service should still be considered somewhat experimental, due to ongoing work to add another MR pipeline, MrBUMP,

[ccp4bb] Philosophical question

2013-03-18 Thread Theresa Hsu
Dear all I have a somewhat philosophical question. Why do all protein sequences start with a methionine (not referring to mature/processed form)? What is so special about methionine and cannot be replaced by other amino acids? Second, how does the ribosome know the first start codon is for

Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

2013-03-18 Thread Soisson, Stephen M
BLASPHEMY! haha From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob Keller Sent: Monday, March 18, 2013 1:22 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List? I don't want to crystallize the protein--I have another

[ccp4bb] Structural biology to support anticancer drug discovery at the NICR, Newcastle University

2013-03-18 Thread Martin Noble
Dear all, As per the attached, we are really pleased to announce up to four positions to join a structural biology team supporting drug discovery in the Northern Institute for Cancer Research and the Chemistry Department, Newcastle University. The structural biology positions, supported by

[ccp4bb] International Conference on Structural Genomics - Structural Life Science, Sapporo, Japan, July 29-Aug 1, 2013

2013-03-18 Thread Terwilliger, Thomas C
Dear Colleagues: On behalf of the organizing committee of the International Conference on Structural Genomics 2013 – Structural Life Science – (ICSG2013-SLS), we cordially welcome you to the conference, to be held in Sapporo, Hokkaido, Japan, July 29th – August 1st, 2013. ICSG2013-SLS is

Re: [ccp4bb] Most Abundant Eukaryotic Membrane Protein List?

2013-03-18 Thread David Waterman
I wouldn't be so sure that HeLa is just XX, or that it should be called garden variety after what I read today. http://www.nature.com/news/most-popular-human-cell-in-science-gets-sequenced-1.12609#/b1 -- David On 18 March 2013 15:08, Jacob Keller j-kell...@fsm.northwestern.edu wrote: Dear

[ccp4bb] How to convert file format from CNS to CCP4

2013-03-18 Thread Wei Feng
Dear all, I have used CNS to calculate the experimental phase of my structure. After Heavy-atom search, Heavy-atom refinement/SAD phasing and SAD Phasing - Density Modification - Selection of Map. Some files outputted: sad_phase2.hkl sad_phase2.sdb density_modify.hkl density_modify.map