Re: [ccp4bb] gelification of a pure protein

2013-04-23 Thread Antony Oliver
To ask a potentially daft question - but why do you need to reduce the salt? You say you are able to purify it, and that it behaves on gel filtration - but only starts misbehaving when you dialyse, and the NaCl is below 100 mM. We've crystallised many proteins, particularly DNA-binding

Re: [ccp4bb] gelification of a pure protein

2013-04-23 Thread Cécile Breyton
Dear Pascal, We have the case of a protein that gelifies without precipitating when bearking overexpressing E. coli cells. We had a look at the sample in electron microscopy and the protein polymerises and forms irreversible and very pretty fibers (explaining the gel). Maybe you want to have

[ccp4bb] DNS Group Leader/Manager – Structural Biology – Crystallography

2013-04-23 Thread jovanna bost
  Position: Group Leader/Manager – Structural Biology – Crystallography   Client:Dart NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company with offices in San Diego, CA.  DNS will discover new technologies and develop new therapies to help maintain cognitive vitality

[ccp4bb] DNS Group Leader/Manager – Structural Biology – Protein Chemistry

2013-04-23 Thread jovanna bost
Position: Group Leader/Manager – Structural Biology – Protein Chemistry   Client: Dart NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company with offices in San Diego, CA.  DNS will discover new technologies and develop new therapies to help maintain cognitive vitality

[ccp4bb] DNS Group Leader/Manager – Structural Biology – Protein Production

2013-04-23 Thread jovanna bost
 Position: Group Leader/Manager – Structural Biology – Protein Production   Client: Dart NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company with offices in San Diego, CA.  DNS will discover new technologies and develop new therapies to help maintain cognitive vitality

[ccp4bb] PX Summer School @ St Andrews 25th-31st August

2013-04-23 Thread Garry Taylor
We are pleased to announce this year's Summer School in Protein Crystallography to be held at St Andrews from 25th – 31st August. Further details and an on-line application form can be found at: http://biology.st-andrews.ac.uk/pxsummer/ Closing date is 31st May. Best wishes Tracey Gloster,

[ccp4bb] stereo set up

2013-04-23 Thread Hena Dutta
Hi, My apology for the off topic question. I purchased a Toshiba Qosmio laptop X875Q7390. This has inbuilt ir emiter. It came with NVIDIA 3D VISION active shutter glasses. This is a 3D vision ready laptop. I can see high quality the 3d stereo using this glass for some given still pictures. I want

Re: [ccp4bb] stereo set up

2013-04-23 Thread Scott Thomas Walsh
Hi Hena, The 3D stereo on PyMOL and Coot are being driven through OpenGL, not Direct X support on your current laptop setup. Scott Scott T. R. Walsh, PhD Assistant Professor University of Maryland IBBR/CBMG 3127E CARB-2 9600 Gudelsky Drive

Re: [ccp4bb] gelification of a pure protein

2013-04-23 Thread Ho Leung Ng
Hi Pascal, Is this a membrane protein? I worked on a GPCR that gelled upon high concentration in a detergent/cholesterol solution. It was reversible upon dilution and ran as a monomer by size exclusion chromatography. Detergent assays showed that detergent was not being concentrated in the

[ccp4bb] question about arginine sepharose 4B

2013-04-23 Thread Jacqueline Vitali
Dear all, Arginine sepharose 4B is an affinity column. If you have used it please let me know where you purchased it. GE Lifesciences used to sell it but discontinued it. I found it in China in the site http://www.pharmaceutical-sales.net and I corresponded with their sales representative