To ask a potentially daft question - but why do you need to reduce the salt?
You say you are able to purify it, and that it behaves on gel filtration - but
only starts misbehaving when you dialyse, and the NaCl is below 100 mM.
We've crystallised many proteins, particularly DNA-binding
Dear Pascal,
We have the case of a protein that gelifies without precipitating when
bearking overexpressing E. coli cells. We had a look at the sample in
electron microscopy and the protein polymerises and forms irreversible
and very pretty fibers (explaining the gel). Maybe you want to have
Position:
Group Leader/Manager – Structural Biology – Crystallography
Client:Dart
NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company
with offices in San Diego, CA. DNS will
discover new technologies and develop new therapies to help maintain cognitive
vitality
Position:
Group Leader/Manager – Structural Biology – Protein Chemistry
Client: Dart
NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company
with offices in San Diego, CA. DNS will
discover new technologies and develop new therapies to help maintain cognitive
vitality
Position:
Group Leader/Manager – Structural Biology – Protein Production
Client: Dart
NeuroScience LLC (DNS) is a privately held Delaware Limited Liability Company
with offices in San Diego, CA. DNS will
discover new technologies and develop new therapies to help maintain cognitive
vitality
We are pleased to announce this year's Summer School in Protein Crystallography
to be held at St Andrews from 25th – 31st August.
Further details and an on-line application form can be found at:
http://biology.st-andrews.ac.uk/pxsummer/
Closing date is 31st May.
Best wishes
Tracey Gloster,
Hi,
My apology for the off topic question.
I purchased a Toshiba Qosmio laptop X875Q7390. This has inbuilt ir emiter.
It came with NVIDIA 3D VISION active shutter glasses. This is a 3D vision
ready laptop. I can see high quality the 3d stereo using this glass for
some given still pictures. I want
Hi Hena,
The 3D stereo on PyMOL and Coot are being driven through OpenGL, not Direct X
support on your current laptop setup.
Scott
Scott T. R. Walsh, PhD
Assistant Professor
University of Maryland
IBBR/CBMG
3127E CARB-2
9600 Gudelsky Drive
Hi Pascal,
Is this a membrane protein? I worked on a GPCR that gelled upon
high concentration in a detergent/cholesterol solution. It was
reversible upon dilution and ran as a monomer by size exclusion
chromatography. Detergent assays showed that detergent was not being
concentrated in the
Dear all,
Arginine sepharose 4B is an affinity column. If you have used it please
let me know where you purchased it.
GE Lifesciences used to sell it but discontinued it.
I found it in China in the site http://www.pharmaceutical-sales.net
and I corresponded with their sales representative
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