Hmmm… is it really (physiological-like) binding, or your protein A is
aggregating/precipitating on the partner? Do you have a good negative control
(a similar protein to which protein A should not bind)? Also, as a general
rule, be careful about your detection method for the pull-down, don't
Dear All
the soon to be released update (via the update mechanism) will address this by
applying the patch to imosflm.tcl.
Charles Ballard
CCP4
On 23 Oct 2013, at 23:35, Andreas Förster wrote:
Hi David,
I've just renamed the ipmosflm in $CCP4/bin to ipmosflm_new and then defined
Dear colleagues,
We are pleased to announce that the 2014 EMBO practical course on
Computational Structural Biology - from data to structure to function
will be held at EMBL-EBI, 7th - 11th April, 2014.
Now in its 5th consecutive year, this unique and highly popular
practical course covers
Dear all,
I was just wondering if anyone has some information or references about the
dimensions of the largest protein crystal ever grown? I am aware that for
neutron protein crystallography one usually needs crystals with mm
dimensions. I have found some information on crystallization under
Very recently (last few months), 3x1x1mm monsters for neutrons. I think
J. Crystal Growth.
On 24/10/2013 16:33, Tobias Beck wrote:
Dear all,
I was just wondering if anyone has some information or references
about the dimensions of the largest protein crystal ever grown? I am
aware that for
I remember seeing an approx (5mm)^3 haemoglobin crystal in the MRC LMB crystal
growing room, and the nucleosome crystals there were almost as big in their
longest dimension.
Bill
On Oct 24, 2013, at 8:33 AM, Tobias Beck tobiasb...@gmail.com wrote:
Dear all,
I was just wondering if anyone
Dear Colleagues, I want to bring the following position to your attention:
Faculty Position in Structural Biology
Purdue University
The Department of Biological Sciences and the Markey Center for Structural
Biology at Purdue University invite applications for a tenure-track faculty
position in
I once grew an oxymyoglobin crystal 1 cm long for neutron diffraction at
Brookhaven. I was very proud of it, but when I got to Brookhaven I was told it
was too big for the beam (!) so I had to use a much smaller one of only 8 mm**3
(Nature 292:81-82 (1981). I still have a few left over that
Dear Tobias,
Take a look at http://dx.doi.org/10.1107/S0108767389012912
The ribonuclease crystal I used to measure the speed of sound, using laser
generated ultrasound, was of volume 129 mm3 ie 7.7x6.2x2.7 mm . David Moss of
Birkbeck College provided it.
Best wishes,
John
Prof John R Helliwell
Following on from John's comment, when I did my PhD at Birkbeck in the
early 2000s, one of David Moss's other PhD students (John Bond) grew some
gigantic (1cm edges) crystals of things like HEWL Myoglobin, which he
then (somewhat perversely) crushed to load into capillaries for powder
diffraction
This is just to inform that if you have a second screen plugged to your Mac and
you want to use Mac OS 10.9, you should consider disabling the “Displays have
separate Spaces” option, or you won’t be able to work with Coot (the window
will keep hiding from you). This is not a Coot bug (and
Also following on from John's comment - back to the times of my PhD I
was repeatedly growing crystals of bacterial formate dehydrogenase (80
kDa) of a size about 7x1.5x1 mm. I thought that was quite normal and
did not even think of making a photo of 'just a protein crystal'.
Victor
Dear all,
The MX beamline at the Alba Synchrotron (Barcelona), BL13-XALOC, is now open
for user proposal applications. Worldwide institutes are eligible for
beamtime, which is awarded upon peer-reviewed proposals. The beamline is
included in the http://www.biostruct-x.eu/ Biostruct-X funding
Hi,
Last spring I visited the Protein Crystallography Station at Los Alamos. On a
shelf, in a capillary in a serious exhibition-quality glass dome, was a crystal
of myoglobin some 50 mm**3, if I remember correctly. I was told it had been
made by Benno Schoenborn some decades earlier and had
Dear Tobias,
There is also this one :- http://dx.doi.org/10.1107/S0021889801007245
In this study we had to use a smaller crystal than the largest ones available
of 125mm3. They were a lovely rhombic dodecahedral crystal habit. Nb we only
published details of the size of the one used. These
Hi everyone,
Recently I’ve got a protein crystal and I did indexing and scaling with a
cubic space group (unit cell 104.115 104.115 104.115 90.0 90.0 90.0). But a
Rmerge value was too high (around 0.5-0.6). So, I tried lower symmetry
space groups and I successfully solved the structure with a
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