Dear all
How to decide for minimum distance between heavy atoms in shelxd esp w.r.t.
S-SAD.
Thanx in advance
Monica
Dear all
I am naive in phasing experiments. CAn anyone please guide how to do the
following:
In a S-SAD for *SHELXD* searches, try various high-resolution cutoffs for
example 2.5 to 5 Å in steps of 0.1 Å. Based on these attempts, use a
high-resolution cutoff at for example 3.8 Å for substructure
Dear Peter,
First a common misconception: your goal should be to get the best possible fit
of your model to your electron density maps. Rfree is only an indicator,
telling you whether you are moving in the right direction.
So in coot, you should look for places where your model does not fit
Dear Monica,
if you are new to phasing I recommend you first use the ``standard''
values in shelx c/d/e, either using one of my tutorials or, even better,
the GUI hkl2map, available at http://webapps.embl-hamburg.de/hkl2map/.
If your native data are about 2A or better, and your structure is a
Dear all,
Right now, we are try to crystallise an protein complex. By all kinds of
efforts, the resolution of the crystal was about 8 angstrom. So we would
like to try dehydration.
The precipitant that our crystal grew was ethanol. Since the evaporation of
the ethanol, our crystal is unstable at
I think what one uses will depend on what one expects to be in the structure
and the resolution and the quality of their diffraction data.
I usually start with 1.8 Å resolution data in case there is chance of having
disulfides. Then 1.9, then 2.4, then 2.0, then 2.6, then 2.2, then 1.85, then
Dear all,
Leiden University, The Netherlands has a tenure track position available in
its Protein Chemistry department.
For details, please see the following site:
Dear Jack,
In this recent review paper (http://www.biomedcentral.com/1472-6807/13/6), I
read about non-catalytic Rossmannn fold proteins which include mitochondrial
transcription factor B (sc-mtTFB) (http://www.ncbi.nlm.nih.gov/pubmed/12897151)
and a t-RNA (1-methyladenosine) MTase from
DprA, from the Streptococcus pneumoniae transformasome, is one, I believe:
http://www.ncbi.nlm.nih.gov/m/pubmed/22904190/
On 22 Apr 2014, at 14:26, Tanner, John J. tanne...@missouri.edu wrote:
Does anyone know of a protein that has a Rossmann dinucleotide binding domain
that does not
Actually, that was the issue. I manually reset the occupancies of the
problematic atoms in the PDB to unity. This solved my problem.
Thanks to Nat and Tom.
Best,
Chris
On 4/21/14, tom.p...@csiro.au tom.p...@csiro.au wrote:
Hello Chris,
This probably isn't your problem, but I once put some
Dear all:
I am facing a display issue in pymol. In my structure, there is a nucleic acid
chain with some monomers which are modified nucleotides built in scketcher of
ccp4 suite. However, When I showed the structure in pymol as cartoon, the
nucleic acid chain was discontinuous where the
Dear all,
The registration deadline to the 2nd Australian Advanced Methods in
Crystallography Workshop has been extended.
Registration deadline: 6th May 2014
Workshop dates: 22-25th June 2014
Website: https://events.synchrotron.org.auhttps://events.synchrotron.org.au/
Contact:
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