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Looks like you're on the twofold axis, which will make interpretation
challenging. Anything you put in may end up being too close to itself in
the neighboring AU. What happens if you put in a water and display the
symmetry?
On Mon, Jun 23, 2014 at 8:17 AM, Shanti Pal Gangwar
Dear Shanti
Looks like you’re looking down the symmetry axis - so this could simply be
‘noise’, or a superposition of two bound ligands on top of each other… what’s
your cryo-protectant?
With regards,
Tony.
- - - - - - - - - - - - - - - - - -
Dr Antony W Oliver
Senior Research Fellow
CR-UK
Dear Shanti Pal,
did you use ethylene glycol as cryoprotectant? It may even be there in
small amounts in your PEG400 solution. As Nicholas and Tony have said, this
could be noise (or could be distorted due to noise) as it's on a 2-fold
axis. From those pictures, it looks to me like one molecule
Dear all,
Is there any standard protocol to know the number of incorporated
seleno-methionine in a protein ??? I am growing one of the protein in
minimal media for phase determination.
I am using mass spectrometry facility to know the incorporated
seleno-methionine but I wanted to know whether I
