Thank you everyone...
On Sun, Aug 17, 2014 at 10:35 PM, Bernhard Rupp (Hofkristallrat a.D.)
hofkristall...@gmail.com wrote:
Also MATTPROB indicates 3 or 4 molecules/asu as likely solutions.
http://tinyurl.com/pe2choc
I do not understand why you propose 16 mol/asu?
The entire unit cell
Dear All,
Greetings and apology for an off topic query. may request you all to kindly
share with me a conventional protocol, by which i can calculate a total
Glutathione content (oxidized + reduced) in an *in-vitro* reaction set up.
all i find over internet are kit-based methods, which turns out
Dear Avinash,
you can use Ellman's reagent (DTNB) for GSH.
Have a look here:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Thiols_and_disulfides
For glutathione you don't have to use a buffer with GuHCl.
HTH, Guenter
Dear All,
Greetings and apology for an off topic query. may
Next MX-proposal application deadline: September 1, 2014 is approaching
BAG proposal applications are now possible
Single 8h-shift beamtime bookings are now possible
We kindly invite new MX-proposals for beamtime applications for the next
beamtime period.
In order to apply for beamtime, please
Dear colleagues,
this is to remind you of our symposium IYCr2014@Innsbruck on 8-9
September 2014 in Innsbruck (Austria), celebrating 100 years of
crystallography.
Registration is still possible, have a look at
http://biocenter.i-med.ac.at/iycr2014 for more information and an
updated program.
Avisek,
Besides the advice that Bernhard and Remy have given you, beware of strange
packing arrangements with low symmetry space groups and higher order oligomers.
We have had several cases in P21 and C2 where tetramers pack with 6 or 8
molecules in the asymmetric unit (ASU), but could not be
(Don't know if this should go to developers or the list. Some users may have
more information, or be interested in the information.)
I'd like to point out what i believe to be errors in the HEC.cif file released
with
CCP4 version 6.2 and later.
HEC is heme c which is regular heme as in
