If you want to test this, set the ARG atom occupancies to 0.0 - calculate
another map, and see what the density looks like - you can flip through the
aRG rotamers using coot.
Eleanor
On 13 February 2015 at 06:12, Dialing Pretty
03f1d08ed29c-dmarc-requ...@jiscmail.ac.uk wrote:
Dear All,
Dear colleagues,
We are pleased to announce the
Sixth edition of the Macromolecular Crystallography School 2015, to be held at
the Institute Química-Física Rocasolano- CSIC (Madrid, Spain).
Dates: May 18 - 23, 2015
Invited speakers and tutors:
Paul Adams, Berkeley, USA
Pavel Afonine,
Dear all,
Sorry for this off topic question. When I started PyMOL (Windows
1.6.0.0), it is defaulted to file type ACNT maps. Is there any way that
I can set the program to default to PDB? Thanks.
Wai
--
Yu Wai Chen, PhDLecturer
King's College London,
Dear CCP4 Users
An update for the CCP4-6.5 series has just been released, consisting
of the following changes
* refmac (all)
- bug fix for occupancy refinement
* imosflm/mosflm
- update to 7.1.2
- bug fix update
* pointless (all platforms)
- update to 1.9.25
- Some fixes for Saint
Dear Community,
Sorry for this off topic question. I am dealing with a non specific DNA
binding protein. In a non radio-labelled EMSA DNA:protein titration
experiment when I EtBr stained the 5% native acrylamide gel, I could see
the DNA control (101 bps) but as the amount of my protein increases
Sudipta,
the link for the cyanin dyes are at the following link
http://www.lifetechnologies.com/us/en/home/references/molecular-probes-the-handbook/nucleic-acid-detection-and-genomics-technology/nucleic-acid-stains.html
Sorry for the wrong link
P
On Fri, Feb 13, 2015 at 1:50 PM, Sudipta
Hi,
On 13 Feb 2015, at 12:31, Yu Wai Chen yu-wai.c...@kcl.ac.uk wrote:
Dear all,
Sorry for this off topic question. When I started PyMOL (Windows 1.6.0.0), it
is defaulted to file type ACNT maps. Is there any way that I can set the
program to default to PDB? Thanks.
Check this link:
What is the charge on your protein? If it is a very positively charged protein
it may not enter the gel. I have found this before in EMSAs but normally at low
protein concentrations it still enters the gel only at the higher concs it does
not. I had to change protein to DNA ratio and also the
