An opening is available in the laboratory of Dr. Stephen Fesik
(http://www.mc.vanderbilt.edu/fesiklab) for the Research Associate position in
structural biology and drug discovery. The candidate must possess a degree in
biochemistry or a related field with 1-2 years of experience in protein
Hi Stephen,
we crystallized several proteins in a glove box placed in a room with
air condition. We checked the temperature all the times and found that
is had been quite stable.
For one protein that crystallized only at 4 deg C, we setup the
crystallization plate within the glove box and
A look at the term scheme might help to understand the absorption edge
structure - pp 286 and Figure 6-30 BMC.
Best, BR
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Keller, Jacob
Sent: Wednesday, March 11, 2015 9:58 AM
To: CCP4BB@JISCMAIL.AC.UK
Hi Jacob:
If you can find a copy of R. W. James, THE CRYSTALLINE STATE VOL II: THE
OPTICAL PRINCIPLES OF THE DIFFRACTION OF X-RAYS. Hardcover – 1965, this gives a
very good (albeit primarily classical) description. Blundell and Johnson also
has a good, slightly more modern, description, which
Hi,
Molprobity is also a nice software to do this kind of analysis - if you
use it as implemented in phenix you also get good visualization in coot.
I also asked the pymol community to create an implementation for pymol,
but I did not follow if somebody took that up.
Also this 'protein
Thanks for all of the responses, there seems to be no real consensus, but I
have discovered:
1 There are several small incubators supplied by - Molecular Dimensions,
Revsci, Centeo
2 Good air conditioning may negate the need for an incubator.
3 A Glove box may not even be necessary depending
Several have mentioned James to me, and I have had and read a copy for quite
some time. I find, however, that the descriptions are more mathematical than
mind-pictures. While math is more agnostic, I find mind-pictures more
prognostic and helpful to me. But I absolutely love James.
Somebody
Dear CCP4BBer's
Apologies for the off-topic post, but the CCP4BB seems to be the best place to
ask about crystallisation.
I am looking to set up crystallisation in an anaerobic glove box and wondered
how other people did this, specifically the crystallisation stage. My initial
thoughts were
Dear Steve,
There is a model of small incubator that fits through the large portal of a
Belle Technology rigid glovebox - as an aside, that’s a good box for
crystallisation and crystal manipulation since you can get the
microscope-mounted side box with a dewar port for rapid and pretty
Dear Steve,
You should not require an incubator if you have good, stable air con. We
routinely grow crystals in plates stored at room temperature in wall mounted
cabinets in our crystallisation lab. Another of the groups here have
experience of growing crystals in an anaerobic box.
On Mar 11, 2015, at 11:13 AM, Keller, Jacob kell...@janelia.hhmi.org wrote:
Somebody sent me a pdf with such a mind-picture which describes the origin of
the anomalous effect as arising from broken centrosymmetry of the anomalous
atoms themselves:
(p. 8) Under normal conditions,
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