Re: [ccp4bb] Off-topic x 2: Chaperone co-expression AND Bac-Mam contract work

Hi, You can find GroESL expressing plasmid and plasmids with different chaperones combinations from prof. Bernd Bukau lab on Addgene: https://www.addgene.org/Bernd_Bukau/ Best Michal 2017-01-20 3:19 GMT+01:00 Patrick Loll : > Hello everyone, > > Please allow me to kill

Re: [ccp4bb] help with Buccaneer

You need go first for some density improvement, see http://ccp4wiki.org/~ccp4wiki/wiki/index.php?title=Automated_phase_improvement_with_Parrot All the best Carlos On 01/20/17 05:10, Vikram Dalal wrote: Hi Everyone, I want to run the Buccaneer. I need Hendrickson-Lattman coefficients for

Re: [ccp4bb] help with Buccaneer

When faced with a program requiring HL coefficients, and having phases from a (partial) model, I used to run a ccp4 program called sigmaa to generate the HL coefficients. I'm not sure if this is theoretically a good idea, but at least it allows the program to run. On 01/19/2017 11:10 PM, Vikram

[ccp4bb] help with Buccaneer

Hi Everyone, I want to run the Buccaneer. I need Hendrickson-Lattman coefficients for it. How I can find Hendrickson-Lattman coefficients? Thanks & Regards,

Re: [ccp4bb] CCP4BB Digest - 18 Jan 2017 to 19 Jan 2017 (#2017-20)

Hi, everyone, I think I found the cause and solution. It was due to the project folder of CCP4i,which was set by me to E:/***/** p reviously, based on which imosflm automatically set its processing folder also as E:/***/. Because disk E: used to be the flash disk on my computer, when I unplug

Re: [ccp4bb] Off-topic x 2: Chaperone co-expression AND Bac-Mam contract work

Hi Patrick: We have used the Clonetech Chaperone Coexpression Kit. That includes GroEL/GroES plasmids. http://www.clontech.com/BT/Products/Protein_Expression_and_Purification/Bacterial_Expression_Systems/Protein_Folding/Chaperone_Plasmid_Set Hope this helps, Alexandra On 1/19/17 9:19

[ccp4bb] Off-topic x 2: Chaperone co-expression AND Bac-Mam contract work

Hello everyone, Please allow me to kill two off-topic birds with one email ‘stone’: 1) Can anyone suggest where I can lay hands on the pREP4-GroESL plasmid for bacterial co-expression of GroEL and GroES? I’ve checked with the original author (Philip Cole), but it’s been decades since he

Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)

Excellent point. I've heard apocryphal stories of someone obtaining beautiful salt crystals (magnesium ammonium phosphate) by trying to crystallize an ATPase with ADP and Mg++ using (NH4)2SO4 as a precipitant. Wait long enough, and the ADP becomes ATP plus AMP, the ATPase takes the 3rd

Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)

Remember that 2xADP can disproportionate into ATP + AMP > On 19 Jan 2017, at 20:54, Panneerselvam, Saravanan > wrote: > > Dear All, > Sorry for the little bit off topic. Is there a possibility for covalent > bond formation between beta phosphate of ADP and

Re: [ccp4bb] Anisotropy and temperature

> Diffraction measurements at a single temperature do not distinguish > between these two components. In principle a series of diffraction > measurements from the same crystal at different temperatures would > allow partitioning the observed vibrational into the two components. > [Burgi (2000)

[ccp4bb] Joint Postdoctoral Position in the Grishin and Chook Labs at UT Southwestern

The Grishin (http://prodata.swmed.edu/ ) and Chook (http://www4.utsouthwestern.edu/chooklab/ ) Labs at UT Southwestern seek a recent PhD graduate for a postdoctoral fellow position to study structural determinants of nuclear

Re: [ccp4bb] Anisotropy and temperature

On Thursday, 19 January, 2017 20:35:14 you wrote: > A PhD student asked me what causes diffraction anisotropy. Quoting from the > Diffraction Anisotropy Server webpage that it is caused by whole-body > anisotropic vibration of unit cells. He asked whether a colder cyrostream > could improve

Re: [ccp4bb] Anisotropy and temperature

The actual vibrations don't exist at 100 K (similar to the case of B-factors/ADPs, sometimes called mildly misleadingly "temperature factors"), but are rather "frozen" where they were when they get cold. So a colder cryo stream would not help. JPK -Original Message- From: CCP4

Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)

On 1/19/17 3:54 PM, Panneerselvam, Saravanan wrote: We observed additional density around ADP that fits perfectly like a gamma phosphate Hello Saravanan At 1.4 Angstrom resolution wouldn't that suggest that you've somehow got ATP in there ? I don't think I understand the other option - were

Re: [ccp4bb] CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)

Dear All, Sorry for the little bit off topic. Is there a possibility for covalent bond formation between beta phosphate of ADP and acetate molecule both are coordinated by divalent metal ions? I am working on a Kinase structure which was crystallized with ADP and in presence of 1M sodium

[ccp4bb] Anisotropy and temperature

A PhD student asked me what causes diffraction anisotropy. Quoting from the Diffraction Anisotropy Server webpage that it is caused by whole-body anisotropic vibration of unit cells. He asked whether a colder cyrostream could improve anisotropy. My answer would be yes, as colder temperatures

[ccp4bb] PhD fellowships in Spain

Dear future PhD student, For prospective PhD students there is the current fellowship call open: https://obrasociallacaixa.org/el/educacion-becas/becas-de-posgrado/inphinit/programme-description This is a competitive call with good conditions (salary, mobility, complementary training),

[ccp4bb] Unique postdoctoral research opportunity in Tromsø, Norway

Dear all, I would like to call your attention to a unique postdoctoral fellowship opportunity in structural chemistry at the UiT The Arctic University of Norway in Tromsø. The project area is "Crystallography, biophysical and cheminformatics studies for next-generation kinase inhibitor

[ccp4bb] Cryosystems series 600

We are having a clear out and have several Oxford cryosystems 600 going spare. They are not in working order, and they are really only for spare parts. Free to a good home, pick up only. Contact me off list if you are interested. If we don't hear within 10 days, they will go in the bin. Johan

Re: [ccp4bb] Off-topic, protein in dye-front (ion front?) on native-PAGE

Try Tricine gel. It is particularly suited for low molecular wt proteins and it will give you very good resolution. Even you can run it overnight at 30 V (16-18 hours). On Thu, Jan 19, 2017 at 9:33 AM, Walt wrote: > Hi, > > I have a small protein (~9 kDa) with acidic pI (~4).

[ccp4bb] 6th Edition of the ISBC2017

Dear Colleague, The Laboratory of Crystallographic Studies is pleased to announce the 6th International School on Biological Crystallization (ISBC2017), to be held in Granada (Spain) during May 29th to Jun 02nd, 2017. ISBC2017 is intended for postgraduate/postdoctoral students and research

Re: [ccp4bb] Off-topic, protein in dye-front (ion front?) on native-PAGE

A) change dye or use pure glycerol to load gel B) change gel pH or add various substances to it (easy to do with the buffer) Artem On Jan 19, 2017 6:34 AM, "Walt" wrote: > Hi, > > I have a small protein (~9 kDa) with acidic pI (~4). > When I run 18% native-PAGE, it appears

[ccp4bb] Off-topic, protein in dye-front (ion front?) on native-PAGE

Hi, I have a small protein (~9 kDa) with acidic pI (~4). When I run 18% native-PAGE, it appears my protein is in the dye front. How can I fix this problem? Changing the pH of separating gel might help? How about gradient native-PAGE? Thank you! Walt

[ccp4bb] Call for MX beamtime proposals at HZB, BESSY II, deadline March 01, 2017

Next MX-proposal application deadline: March 01, 2017 is approaching http://www.helmholtz-berlin.de/user/beamtime/proposals/index_en.html Hereby we would like to invite the submission of new proposals for MX-beamtime at the HZB-MX beamlines for the next beam time period (08/2017-01/2018). In

Re: [ccp4bb] *** WARNING SUSPECTED VIRUS, SPAM or SCAM *U* [ccp4bb] error in startup script

Hi It looks like you've got the Windows environment variable "MOSDIR" set to "E:\" when running CCP4i - I have no idea how that would happen; it's normally set to something like C:\MOSDIR. If you haven't got an E:\ drive that would be the root of your problem... Have a look in the list of

[ccp4bb] AW: [ccp4bb] on space group

Dear Smith, I think your question was clear, and the answer you got was clear as well. However, I think the question you asked was not the right question. You want to use a particular phrase to describe your crystal packing and you want the CCP4BB to endorse this. When the answer was negative,