[ccp4bb] Computational Crystallography Newsletter, Volume 8, Number 1

I am pleased to announce the publication of the latest issue of the Computational Crystallography Newsletter: http://www.phenix-online.org/newsletter/ A listing of the articles and short communications is given below. Please note that the newsletter accepts articles of a general nature of

[ccp4bb] AW: [ccp4bb] How to determine the concentration of biotinylatedpeptide?

very good point! you need lots of protein and it has to be pure (meaning also minimal buffer, salts and stuff) but it worked pretty well in our hands (when we were trying to measure the extinction coefficient of phytochrome). incidentally, I think that the notion of quantitative amino acid

[ccp4bb] Postdoctoral researcher in Structural Biology

Dear colleagues, The Deindl group (www.deindl-lab.com) is recruiting a highly motivated postdoctoral researcher with a natural talent and strong motivation to identify and solve scientific problems. He/she will carry out cryo- EM or X-ray crystallographic and

Re: [ccp4bb] How to determine the concentration of biotinylated peptide?

These suggestions are all possible, but why not simply lyophilize it into a tared tube and weigh it out? On Mon, Feb 6, 2017 at 12:28 PM, Alex Lee wrote: > Thank you all for your suggestions! > > On Mon, Feb 6, 2017 at 5:53 AM, Artem Evdokimov

[ccp4bb] Employment opportunities at Charles River Labs

Dear colleagues, I would like to draw your attention to the following employment opportunities within Charles River Labs' Structural Biology group, based in Saffron Walden, UK: https://jobs.criver.com/job/saffron-walden/protein-crystallographer/4665/3817382

[ccp4bb] Position available for crystallographer with Bruker AXS – Business Development Scientist Structural Biology

Dear CCP4 community, A position is available for an experienced crystallographer to join Bruker AXS in a scientific support role in the Structural Biology Business. The successful candidate will join a highly motivated team, providing scientific and application support in the fields of

[ccp4bb] PhD position available - PDZ-net european training program

Posted on behalf on Prof. Stefan Knapp. We currently have a PhD position available within Prof. Stefan Knapp group at Goethe-university Frankfurt: The project is embedded into a highly interactive European training program involving internationally well recognized laboratories and companies.

Re: [ccp4bb] How to determine the concentration of biotinylated peptide?

Hi, In addition to HABA dye assay (which will work great but will also be fooled by any biotin that is not conjugated) you can do: * quantitative MS * TLC * HPLC * elemental analysis * https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614710/ biotin catalysis of the N3- + I3- reaction (also fooled

Re: [ccp4bb] !SPAM: [ccp4bb] P622 xds index process with large unit cell

Dear Peter, if this is indeed your entire XDS.INP, you asked XDS to refine all parameters except the axis during the integration step. This may have led to a drifting away from the input parameters. Try e.g. REFINE(INTEGRATE)=CELL ORIENTATION BEAM to fix the detector distance during

[ccp4bb] P622 xds index process with large unit cell

Dear All, I got a data set from Diamond recently with space group of P622 and cell parameters: 87 87 629; 90 90 120. I can process the data using mosflm at home, However, XDS could not do with error saying " !!! ERROR !!! CANNOT INTERPRETE LATTICE BY THE GIVEN UNIT CELL". Can anyone advice me

Re: [ccp4bb] on the resoution of crystal

6-Feb-2017 Dear Pavel & Tim, For a very good visualization & description of “Resolution”, please see the Proteopedia page created primarily by Eric Martz at: http://www.proteopedia.org/w/Resolution it includes a pointer to James Holton’s movie showing an Electron Density Map vs. Resolution (with

[ccp4bb] AW: [ccp4bb] Unknown blob extended from catalytic serine residue

Dear Sharifah, Was no protease inhibitor used during crystallization, or during the whole purification process? E.g. when a protease inhibitor cocktail was added during cell-lysis, a protease inhibitor may irreversibly have modified your serine. If your protein is a protease, it may have