[ccp4bb] Crystallization suggestion for antigen-Fc complex

Dear CCP4 members, I am trying to crystallize an antigen-bound to the Fc fragment of an antibody. Is Fc fragment difficult to crystallize compared to Fab? Are there any commercial screens widely known to crystallize Fc fragment or antibodies? I would appreciate if you can share any experience

Re: [ccp4bb] B-factors/Occupancies Versus "Local Resolution"

On Monday, 06 March, 2017 19:54:23 Keller, Jacob wrote: > Dear Crystallographers (and cryo-EM practitioners,) > > I do not understand why there is a discrepancy between what crystallographers > use to models disordered regions (b-factors/occupancies) and what the cryo-EM > world uses ("local

[ccp4bb] B-factors/Occupancies Versus "Local Resolution"

Dear Crystallographers (and cryo-EM practitioners,) I do not understand why there is a discrepancy between what crystallographers use to models disordered regions (b-factors/occupancies) and what the cryo-EM world uses ("local resolution.") I am tempted to say that "local resolution" is a

[ccp4bb] 8th workshop on Neutron Scattering Applications in Structural Biology

Second announcement 8th Workshop on Neutron Scattering Applications in Structural Biology Oak Ridge, TN. June 5 - June 9, 2017 Detailed information can be found on the workshop web page: https://conference.sns.gov/event/66/ Application deadline: April 22, 2017 The workshop on Neutron

[ccp4bb] Phasing and Model Building satellite meeting in Hyderbad

Dear All from http://www.iucr2017.org/program/satellite-meetings/ Phasing and Model Building Satellite meeting in Hyderabad at CSIR-IICT on 20 – 21 August 2017 This one and half day satellite meeting on the CCP4/Phenix will provide an overview of Phasing with Anomalous Diffraction, Molecular

Re: [ccp4bb] Crystallization Screens

Dear FBDD Evangelist, As Andreas mentioned, Emerald is now Rigaku Reagents. We still offer this screen, but under different name (Wizard pH Buffer screen). Here’s a link: http://www.rigakureagents.com/p-11-wizard-ph-buffer-96-well-block-plate.aspx Best, Angela From: CCP4 bulletin board

Re: [ccp4bb] Crystallization Screens

Many thanks to all, I was able to obtain the screen conditions quite quickly, thanks to you. Good to know that there still is some information out there about these discontinued screens, which is good. You never know how many of these old screens might still be around (worldwide!) and be

[ccp4bb] PhD position available - Sussex University

Dear All An exciting PhD studentship, at the intersection between Myeloma Research, Structural Biology and Drug Discovery, is available in the School of Life Sciences at the University of Sussex. Multiple myeloma is an aggressive and incurable cancer of plasma cells, with rising incidence

Re: [ccp4bb] Crystallization Screens

In general, at Formulatrix we have a lot of these discontinued screens on record, albeit in RM-XML format (for import into RockMaker). I'd be happy to provide .CSV exports of these if needed. Send requests to me offline. Flip On 3/6/2017 11:18, Hernani Silvestre wrote: many thanks! On 6

Re: [ccp4bb] Crystallization Screens

many thanks! On 6 March 2017 at 10:16, wrote: > ​Hi Hernani, > > > If you look at c6.csiro.au - use the guest account to log in, then > select (via pulldown menus) the report "Single Screen Contents", then "A > Vendor" in answer to the question "specify" then "Rigaku

[ccp4bb] Vacancy: structural biologist with cryo-EM expertise at GSK closing date 24th March

Job-posting - Seeking structural biologist with cryo-EM expertise at GSK A vacancy for a structural biologist with cryo-EM expertise has opened up within the UK structural and biophysical sciences group at GlaxoSmithKline R Joining an experienced multi-disciplinary group, this is an exciting