[ccp4bb] crystallization screen for protein-protein complex

2017-05-20 Thread Hena Dutta
Dear Members, I am trying to crystallize a protein-protein complex. Both are soluble proteins and form complex as observed in FPLC either co-purifying or separately purifying and then mixing with equi-molar ratio. Looking for suggested screens to try for crystallization. So far tried with Qiagen

Re: [ccp4bb] NAD dihedral for C2N-C3N-C7N-N7N

2017-05-20 Thread Jorge Iulek
Thanks Victor, Dale and John for inputs concerning this question. Yes, always to check the cif files. I will look carefully at the hydrogen bond network, though both conformations might be possible; right now I am testing both and I will check better

[ccp4bb] Off-topic: 3D printing tools for molecular models

2017-05-20 Thread Paul Paukstelis
Sorry for the somewhat off-topic post. After getting interested in 3D printing I quickly found that making nice ball-and-stick objects was very difficult to do on the typical fused filament printers most people can afford. This is largely because of the amount of support structures needed for