Re: [ccp4bb] mystery feature near a PLP substrate

2017-05-24 Thread Avinash Punekar
DMSO may fit nicely there.

Re: [ccp4bb] mystery feature near a PLP substrate

2017-05-24 Thread Karolina Michalska
Hi, The Lys-PLP adduct looks like a perfectly happy internal aldimine. I do not see any indication of a covalent bond between PLP and the density in question indicating any other intermediate. Which, to my knowledge, would be rather hard to get accidentally. I do not know distances between these

Re: [ccp4bb] mystery feature near a PLP substrate

2017-05-24 Thread SR Bharath
It is most likely amino acrylate, the intermediate in the reaction of PLP with amino acids. On Wed, 24 May 2017 at 22:44, Francis Reyes wrote: > Fidget spinner? > > https://en.m.wikipedia.org/wiki/Fidget_spinner > > F > > On May 24, 2017, at 10:16 AM, Eleanor Dodson

Re: [ccp4bb] mystery feature near a PLP substrate

2017-05-24 Thread Francis Reyes
Fidget spinner? https://en.m.wikipedia.org/wiki/Fidget_spinner F > On May 24, 2017, at 10:16 AM, Eleanor Dodson > wrote: > > Any ideas? >

Re: [ccp4bb] mystery feature near a PLP substrate

2017-05-24 Thread Carlos Pla Prats
Have you tried modeling a fidget spinner there? Carlos On Wed, May 24, 2017 at 4:33 PM, Soisson, Stephen M < stephen_sois...@merck.com> wrote: > This looks suspiciously like Cartoon moose-head. Is it possible that you > have contamination in your buffers? We once had this problem when using >

Re: [ccp4bb] mystery feature near a PLP substrate

2017-05-24 Thread Soisson, Stephen M
This looks suspiciously like Cartoon moose-head. Is it possible that you have contamination in your buffers? We once had this problem when using Canadian water. [Related

[ccp4bb] post-doc in RNA enzymology in Paris (France)

2017-05-24 Thread Beatrice Golinelli
A postdoctoral position is available in Beatrice Golinelli-Pimpaneau’s group at the Collège de France, 75005 Paris, France. In Marc Fontecave’s lab (https://www.college-de-france.fr/site/chimie-processus-biologiques/index.htm), the « RNA enzymology » group aims at understanding the structural

Re: [ccp4bb] XDS on Windows

2017-05-24 Thread Kay Diederichs
Hi Jan, Rob, it is documented in XDSwiki at http://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Installation#Windows - I just added the hints concerning the Creators Update (can anybody verify that OpenMP parallelization works with that??). best, Kay On Tue, 23 May 2017 14:06:54

[ccp4bb] EM Facility Manager position - London

2017-05-24 Thread Zhang, Xiaodong
The Section of Structural Biology within the Department of Medicine along with its partners (The London Consortium for cryo-EM) is seeking to recruit a Facility Manager for a new high resolution Electron Microscopy Facility. Imperial College London, in partnership with the Institute of Cancer