Dear all,
Posted on behalf of Peijun Zhang...
THREE Wellcome Trust funded postdoctoral fellowships are available in
Peijun Zhang lab at the University of Oxford (https://www.ndm.ox.ac.uk/prin
cipal-investigators/researcher/peijun-zhang
Dear Thomas,
I do all my experiments in a 384 well, non-binding plates. I have a salt
concentration of 50 mM NaCl, with BSA. i will try the other suggestions as
well.
>From a couple of experiments, I could determine the Kd to be approx. 10 nM.
Thanks for all the suggestions!
On Fri, Jul 21,
Dear Didier and Opher,
The difference in polarities can reach upto 60 units. I have tried
concentrations between 1-5 nM DNA. Maybe I will give higher concentrations
a shot.
Thanks!
On Fri, Jul 21, 2017 at 4:02 PM, Didier Spittler
wrote:
> Hello,
>
> What is your
Dear Julius,
That is a good suggestion. I will definitely try this.
Thanks!
On Fri, Jul 21, 2017 at 3:41 PM, Rabl, Julius wrote:
> Dear Mohammad,
> your buffer is key to success in biophysical measurements. While your
> protein may be totally fine in standard buffer, the
May i ask, whether the fluoresnce anisotropy method was reliable enough to
determine the stoichiometry of a protein complex?
发自网易邮箱大师
在2017年07月22日 03:44,Phoebe A. Rice 写道:
You might also be getting aggregation.
If you do an old-fashioned EMSA ("gel shift") assay, does it hang up in the
well?