Dear Colleagues,
We recently acquired HDX data on a target, which basically gives us %change
per residue over the entire primary sequence. Is there a simple way to
reassign the B-factor column of my PDB with those %change numbers so I can
then easily color the molecular surface? I hope the
Hi Radhika,
R-factor value is almost useless unless you know the resolution (which you
did not tell us): 26% is ok for 3A resolution and is nonsense for 1A, for
example. The Rfree-Rwork gap is obviously large, suggesting sub-optimal
refinement strategy. Try optimizing weights, let program update
Hello All,
I am currently working on the structure of a DNA protein complex. The data
has been processed in space group P1 (53.042 59.527 78.526 105.24
98.03 106.99 P 1, Rpim 11.7%). At this stage I have almost 85% model is
complete but my R/Rfree is stuck as 26%/34%.
I have some concerns
I take it you mean for some semisynthetic approach? One solution that springs
to mind would be to get one or both of the azide/alkyne “click” proline
derivatives (e.g.
http://www.sigmaaldrich.com/catalog/product/aldrich/713643?lang=en=GB or
Sorry for being a bit off-topic, but I’m looking to transform the catalytic
properties of a protein I’m working on by adding an aromatic moiety on one of
its prolines. However none of the proline derivatives I find commercially are
really interesting. Does anyone know what would be the cheapest
Dear all,
Could you pass the following announcement on to suitable candidates (including
yourselves, of course) ?
Thank you.
Fred. Vellieux
Fred. Vellieux (Ph.D., HDR)
Centre of Molecular Structure
Institute of Biotechnology
Czech Academy of Sciences, BIOCEV
Prumyslova 595
252 50 Vestec
Czech
Applications are invited for a joint postdoctoral position in the Department of
Biomedical Engineering and the Medical School of Washington University in St.
Louis. Washington University has a tradition of excellence in medical science
and a stimulating academic environment. Weikai Li and
Dear Jacob,
The big advantage of microscopes (whether using electrons or X-rays) is of
course that you 1) do not need crystals and 2) get phase information. However,
aligning an extremely large number of single molecule images is non-trivial and
this is the reason it is still very hard, if not
Please note that the Early bird deadline for the forthcoming CCP4 Study Weekend
in January 2018 is 19th November 2017 i.e. this Sunday.
After this date, no standard student bursaries will be available and the
registration fee will increase from £240 to £290.
Register now before it's too late!
