Dear All,
I've recently crystallized a protein under a condition containing 1.1 M
ammonium tartrate. (I was able to get a few other hits but only these
crystals diffract below 2.5 A). Upon refinement, it appears that the
electron density in the active site shows a mixture of tartrate and the
Sadly Rafal is right the unit cell dimensions don't make sense - either the
space group is wrong or the contents have been digested before crystallization.
The size of the overall unit cell is ~21 x 23 x 43. Given a (DNA-centric but
close enough) view that a duplex is ~25A wide and there are
Hi everyone,
I have a problem with Crank2 via ccp4i2. The program went through SHELXC/D but
it failed in Bp3 with error message as shown below. May I know what is the
problem?
Thanks for the help.
Best regards
HK
-ERROR- CTaskCrank2:0 Error in wrapper crank2 0.02::
The input objects for
?Hi,
I have seen this type of behavior happen often, albeit in phasing runs with
Buccanneer and/or Phenix. Check the model statistics immediately after phasing,
and compare them to the model statistics after refinement in Refmac. When I
have observed this phenomenon, it's likely due to the
"re-installing" the xds package entails:
- downloading from http://xds.mpimf-heidelberg.mpg.de/html_doc/downloading.html
- the installation is also described there!
- unpacking, typically "tar xvf XDS-INTEL64_Linux_x86_64.tar.gz" on Linux, or
"tar xvf XDS-OSX_64.tar.gz" on Mac
- copying (or
I really need more information to comment properly.
However just check that you are using the same reflections for both Rfree
calculations.
An Rfree of 26% seems low to me from a Se met phased structure at 2.1A -
there are usually small corrections to be made, What is the Rwork?
Maybe attach the
