Re: [ccp4bb] cryo-EM

Hi Xavier, > is there some kind of general trend if not consensus as how to refine > cryo-EM structures? > Not really consensus, but my clearly biased contribution to the topic: I'd say basic common sense for refinement applies: - optimally sharpen the map (

[ccp4bb] cryo-EM

Dear Xavier, in addition to Rob and Israel's excellent suggestions you may also want to have a look at Garib's account in Chapter 11 here: https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/579 Best wishes, Arjen On Friday, 31 August 2018, F.Xavier Gomis-Rüth wrote: > Dear

Re: [ccp4bb] coiled-coil "degree"

As a matter of fact, no, it is not. But it must be available soon! Yours, Jorge On 08/30/2018 01:13 PM, Pius Padayatti wrote: this sounds like an ABC transporter structure just a guess On Tue, Aug 28, 2018 at 6:03 AM, Jorge Iulek > wrote: Dear all,    

Re: [ccp4bb] cryo-EM

Dear Xavier, dear all, good points indeed. I guess the aim is to refine an atomic model against a cryo-EM map so that the atomic model describes the experimental data well, aiming for a good final geometry, refined B-factors etc. of the atomic model. Here is a description of what we recently

Re: [ccp4bb] cryo-EM

Dear Xavier, I recommend having a look at a few of the articles that were published in the special issue of Acta Cryst D from June this year - Proceedings of the third CCP-EM Spring Symposium. https://journals.iucr.org/d/issues/2018/06/00/ Best regards, Rob -- Original Message --

[ccp4bb] cryo-EM

Dear all, is there some kind of general trend if not consensus as how to refine cryo-EM structures? What do people do for maps in the 3-4Å range, pure RSR against the untouched map and that's it? Reciprocal space refinement against the backtransformed structure factors from the map but

[ccp4bb] EMBL Group Leader Positions in Cryo-EM and Systems Biology at EMBL Heidelberg, Germany

Group Leader positions in in Cryo-EM and Systems Biology in the Structural and Computational Biology (SCB) Unit, EMBL Heidelberg, Germany. Application closing date: 7 October 2018. Interviews are planned for 22/23 November 2018. The Structural and Computational Biology (SCB) Unit at EMBL

[ccp4bb] Postdoc positions in structural biology and single-molecule biophysics

Dear all, We’re currently looking to hire two postdoctoral researchers with a background in cryo-EM, X-ray crystallography, or single-molecule biophysics to join our ERC-funded research project at SciLifeLab Uppsala/Stockholm (deindl-lab.com). Our group utilizes