Hi,
Sorry for unrelated email. We are working on a protein however much of it
is unfolded when expressed in *E coli*. I was wondering if some one can
help us by providing chapronin constructs (DNA J and K, GroEL, GroES or any
other).
Many thanks!
Regards
Dr Muhammad Imran
Assistant Professor
Hi Marta,
1-- There is always a possibility of forming detergent/lipid (cholesterol
in your case) -containing crystals combined with other small molecule(s)
(organic or not).
Although you did not specify the width of the oscillation used to collect
the frame displayed in your mail (was it 45
I am forwarding this for my brother:
https://job-search.astrazeneca.com/job/gothenburg/postdoc
-fellow-in-artificial-intelligence/7684/9616027
***
Markus Seeliger
Associate Professor
Department of Pharmacological Sciences
Stony Brook
Hello All,
I have an Post-Doc opening in my lab for an X-ray crystallographer to support
our drug development efforts. Please see the ad
(https://jobs.sciencecareers.org/job/485983/post-doctoral-research-associate-in-x-ray-crystallography/)
for more information or contact me at
Dear Refmac developers,
I am trying to reproduce some successful refmac runs but cannot get the
previous results despite playing around with the parameters.
I did those runs in Feb 2014 (Refmac 5.8.0049) but unfortunately have
overwritten the log files in a backup. I still have the pdbs, mtz
Dear all,
Applications are now open for the 2019 edition of RapiData at SSRL. RapiData is
a practical course in macromolecular X-ray diffraction data collection, data
processing and structure solution. The aim of the RapiData course is to educate
and train young scientists in data collection
Hi Firdous,
For salt conditions try malonate:
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2356-8. Epub 2003 Nov
27.
Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown
macromolecular crystals.
Holyoak T1, Fenn TD, Wilson MA, Moulin AG, Ringe D, Petsko GA.
