[ccp4bb] Postdoctoral Positions in Engineering Ion Channels and Transporters

PostdoctoralPositions in Engineering Ion channels and Transporters   Two postdoctoral positions are availablein the Valiyaveetil group at Oregon Health and Science University in Portland,Oregon. The Valiyaveetil group investigates structure and function of ionchannels and transporters

Re: [ccp4bb] E. coli BirA biotin ligase expression/purification

Dear Gloria, I think that the most economical biotinylation manner is in vivo, by co-expression of a tagged protein with BirA. One can PCR amplify the BirA cDNA from E. coli and clone it into plasmids for any expression system and sub-cellular localization needed. Ready to use BirA expression

Re: [ccp4bb] E. coli BirA biotin ligase expression/purification

Dear Gloria, This is the basic protocol we follow. Hope it helps Arnaud Gautier and Marlon J. Hinner (eds.), Site-Specifi c Protein Labeling: Methods and Protocols, Methods in Molecular Biology, vol. 1266, DOI 10.1007/978-1-4939-2272-7_12, © Springer Science+Business Media New York 2015

[ccp4bb] E. coli BirA biotin ligase expression/purification

Dear friends in crystallography, I know this seems unrelated, but it really isn't ... please forgive me. We are trying to use the Avitag/BirA system to specifically biotinylate target proteins in an economical manner. Are any of you purifying the BirA enzyme in your lab for biotinylation and if

Re: [ccp4bb] off topic: microscope in glove box

Short version: It should be OK, especially since the vacuum is transient and not particularly 'strong*' :) Long version: if this is an older microscope there may be further delamination of optically bonded components if air is already admitted between glass planes (i.e. the optical cement is worn

[ccp4bb] off topic: microscope in glove box

Dear all, I want to put one of our microscopes into the glove box. Does anybody know whether some parts of the microscope optics do not like vacuum in the air lock ? Thanks and best regards, Guenter To unsubscribe

[ccp4bb] PhD position in Structural Biology - Biozentrum, University of Basel; Switzerland

PhD student, 100%, Structure-function analysis of toxin-antitoxin systems in the human pathogen Pseudomonas aeruginosa. The Biozentrum of the University of Basel is one of the world’s leading Life Sciences Institutes with over 30 research groups and 500 employees that research how molecules and

[ccp4bb] PhD and postdoc position – Humboldt-Universität Berlin Germany

Dear colleagues, We are looking for a PhD candidate (m/f/d) and a Postdoctoral researcher (m/f/d) to strengthen our structural biology/biochemistry team at the Humboldt-Universität zu Berlin (HU). Our research focuses on the biochemical principles how archaea and bacteria use carbon monoxide

Re: [ccp4bb] CCP4 Computational Scientist

Dear all, Here's a reminder that CCP4 is looking for a computational scientist to investigate corrections for electron diffraction data. Job details are below. Best wishes -- David On Mon, 23 Sep 2019 at 13:20, Eugene Krissinel wrote: > Dear All, > > CCP4 is looking for computational