Thank you Pavel, responses inline below. There are indeed a lot of
possible approaches, and my experience is far from complete, but I will
share my impressions.
On 8/15/2022 8:15 PM, Pavel Afonine wrote:
Hi James,
I like your approach with dummy atoms and occupancy refinement.
Dealing with
Hi Gergő,
Thank you for discussing it in detail. It is a great help. Based on the
curve fit, we also believe that the two proteins bind independently to the
DNA duplex with the same affinity. The DNA indeed contains 2 sites for
protein binding. As far as the dimerization of protein is concerned,
I am not a proper chemist, but I remember that during my college classes my
professor emphasized that ^x is a measured value and not really related to
stoichiometry. If someone has something to add…
Best
Rafael Marques da Silva
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel
Hello everyone!
Hope this email finds you well. I have an off-topic question regarding ITC
binding studies, which was asked by a reviewer.
We performed an ITC binding study (using Affinity ITC, TA Instruments) to
evaluate protein-DNA interaction which resulted in a perfect sigmoidal
curve.
Dear all,
the next MX-proposal application deadline: September 01, 2022 is approaching
NEW: REMOTE OPERATION IS NOW THE PREFERRED MODE AT BLs14.1 AND 14.2.
PARTICPATION IN REMOTE TRAINING COURSE IS REQUIRED.
As usual, all proposals will be handled by our electronic user portal GATE,
