[ccp4bb] Imidazole maleate

2010-02-12 Thread Ankit Gupta
Hello, I have been using Imidazole maleate pH 5.5 buffer from Axygen Biosciences. Axygen Biosciences discontinued selling crystallography reagents. Does anyone know about the formulation of Imidazole maleate or anyplace that sells this reagent? Thanks! Regards, Ankit

[ccp4bb] SOLVE help

2010-02-05 Thread Ankit Gupta
Hello, I have a SeMet-SAD dataset (at Br edge : 2.7 A) and a 2 wavelength MAD dataset (inflexion and remote : 3 A). The SAD dataset when searched for heavy atom sites with a native dataset (SIRAS) gives a figure of merit of 0.26 and Z-score of 28. The MAD dataset by itself gives a low figure of

[ccp4bb] SOLVE help

2010-02-05 Thread Ankit Gupta
Hello, I have a SeMet-SAD dataset (at Br edge : 2.7 A) and a 2 wavelength MAD dataset (inflexion and remote : 3 A). The SAD dataset when searched for heavy atom sites with a native dataset (SIRAS) gives a figure of merit of 0.26 and Z-score of 28. The MAD dataset by itself gives a low figure of

[ccp4bb] SOLVE help

2010-02-05 Thread Ankit Gupta
Hello, I have a SeMet-SAD dataset (at Br edge : 2.7 A) and a 2 wavelength MAD dataset (inflexion and remote : 3 A). The SAD dataset when searched for heavy atom sites with a native dataset (SIRAS) gives a figure of merit of 0.26 and Z-score of 28. The MAD dataset by itself gives a low figure of

[ccp4bb] SIRAS Phasing - SOLVE/RESOLVE

2009-11-11 Thread Ankit Gupta
Hello, I am currently working on a problem by SIRAS. I have a native and an isomorphous SeMet dataset. I have been trying to use SOLVE/RESOLVE by modifying the MIR file. The problem I am having is with the input file. Can two separate files be used as input, one native.mtz(scaled amplitudes) and

Re: [ccp4bb] Axygen MS-100 plate sealer: user comments?

2009-07-28 Thread Ankit Gupta
performance in pressure sealing crystallization plates and/or in heat sealing deep well blocks. Any user input would be greatly appreciated. Thanks in advance, Chris. -- Ankit Gupta, PhD candidate Dept of BCH/BPH University of Rochester 601 Elmwood Avenue, Box 712, Rochester, NY-14642