Hello,
I have been using Imidazole maleate pH 5.5 buffer from Axygen Biosciences.
Axygen Biosciences discontinued selling crystallography reagents. Does
anyone know about the formulation of Imidazole maleate or anyplace that
sells this reagent?
Thanks!
Regards,
Ankit
Hello,
I have a SeMet-SAD dataset (at Br edge : 2.7 A) and a 2 wavelength MAD
dataset (inflexion and remote : 3 A). The SAD dataset when searched for
heavy atom sites with a native dataset (SIRAS) gives a figure of merit of
0.26 and Z-score of 28. The MAD dataset by itself gives a low figure of
Hello,
I have a SeMet-SAD dataset (at Br edge : 2.7 A) and a 2 wavelength MAD
dataset (inflexion and remote : 3 A). The SAD dataset when searched for
heavy atom sites with a native dataset (SIRAS) gives a figure of merit of
0.26 and Z-score of 28. The MAD dataset by itself gives a low figure of
Hello,
I have a SeMet-SAD dataset (at Br edge : 2.7 A) and a 2 wavelength MAD
dataset (inflexion and remote : 3 A). The SAD dataset when searched for
heavy atom sites with a native dataset (SIRAS) gives a figure of merit of
0.26 and Z-score of 28. The MAD dataset by itself gives a low figure of
Hello,
I am currently working on a problem by SIRAS. I have a native and an
isomorphous SeMet dataset. I have been trying to use SOLVE/RESOLVE by
modifying the MIR file. The problem I am having is with the input file. Can
two separate files be used as input, one native.mtz(scaled amplitudes) and
performance in pressure sealing crystallization
plates and/or in heat sealing deep well blocks. Any user input would be
greatly appreciated.
Thanks in advance,
Chris.
--
Ankit Gupta, PhD candidate
Dept of BCH/BPH
University of Rochester
601 Elmwood Avenue, Box 712,
Rochester, NY-14642