Respected All,
Thanks for your valuable suggestions and inputs.
with regards,
Harsh
On Thu, Mar 21, 2013 at 7:38 AM, Bosch, Juergen jubo...@jhsph.edu wrote:
Yep,
mostly you should stay away from Tris as this is the worst buffer system
when playing with temperature changes. Tris for
Sorry for a simple and non-CCP4 question.
I have determined the structures of three different mutants of a
thermostable protein by X-ray crystallography method. I feel that Mg2+ has
a role in protein stability.
So I want to perform a thermal denaturation study by CD spectroscopy both
in
