Dear All ,
Could anybody suggest the best possible manner to measure the halflife time
of a protein-ATP complex? Our protein binds to ATP and we would like to
measure its half life time after it is bound to radiolabelled ATP. Looking
forward for your valuable suggestions.
Best wishes,
Jan
Dear All,
I am interested in cross-linking of a protein molecule with a complex
(isolated). The protein is expressed recombinantly from E.coli and we are
would like to precisely map the residues involved in interaction. So far,
all other crosslinking methods (targeting amines etc.) have not
Dear All,
I will be grateful to your suggestions about Iron-Sulfur cluster protein
purification. My colleague has some problems with the purification and it
seems that the iron-sulfur cluster might be deformed, and protein is
aggregated. The purification is done under aerobic (normal condition),
Dear all,
I am working on a protein where I have to stabilize the closed conformation
of the protein using disulphide bond. The strategy to design the cysteine
mutants is based on the molecular dynamic simulations, and accordingly the
residues were chosen. The ultimate goal is to trap the ligand
Dear All,
I wonder whether it is possible to get some recommendations/suggestions for
a new set of powerful heavy atom clusters used to phase macromolecular
complexes. Additionally, is anybody familiar whether
Pentamethylcyclopentadienyliridium (III) chloride dimer is soluble in water?
Your
