Re: [ccp4bb] suitable buffer for CD studies

2013-03-20 Thread Kavestri
Sent from my iPad On 20/03/2013, at 7:59 PM, Harsh Bansia spideysp...@gmail.com wrote: Sorry for a simple and non-CCP4 question. I have determined the structures of three different mutants of a thermostable protein by X-ray crystallography method. I feel that Mg2+ has a role in protein

Re: [ccp4bb] suitable buffer for CD studies

2013-03-20 Thread Kavestri
concentrations, I routinely use NaF as an alternative. It is transparent in the wavelength range relevant for far UV CD spectral collection. Kavestri Y. Kingston Laboratory - Structural Biology Group University of Auckland On 20/03/2013, at 7:59 PM, Harsh Bansia spideysp...@gmail.com wrote: Sorry