Re: [ccp4bb] Co-purified ligand present in protein crystal

Wenhe Potentially, mass spectrometry in combination with electron capture dissociation and/or collision-induced dissociation might be helpful. See doi: 10.1007/s13361-013-0662-5. Best wishes, Klaus === Dr. Klaus Fütterer

Re: [ccp4bb] FW: [ccp4bb] Fwd: [ccp4bb] just out of totally idle curiosity ...

I would concur with Ed. I can understand the emotions, but political shouting should be done elsewhere. Let’s remain civil. Klaus === Dr. Klaus Fütterer Deputy Head of School Undergraduate Admissions Room 717, Biosciences

Re: [ccp4bb] comparisons - views

=== Dr. Klaus Fütterer Deputy Head of School Undergraduate Admissions Room 717, Biosciences Tower School of Biosciences P: +44-(0)-121-414 5895 University of Birmingham E: k.futte...@bham.ac.uk

Re: [ccp4bb] SAXS facility and Cryo EM facility

=== Dr. Klaus Fütterer Deputy Head of School Undergraduate Admissions Room 717, Biosciences Tower School of Biosciences P: +44-(0)-121-414 5895 University of Birmingham E: k.futte...@bham.ac.uk Edgbaston

[ccp4bb] Intergrown crystals

of potential ligands/substrates Any suggestions for tricks that we haven't thought of so far? Thank you. Klaus === Dr. Klaus Fütterer School of Biosciences P: +44-(0)-121-414 5895 University of Birmingham

Re: [ccp4bb] Best compounds for heavy atom soaks

Did anybody mention native gel electrophoresis to select suitable HA ions? Worked for us really nicely in a situation were speed was essential. Here's a reference: PMID 14646083 Klaus === Dr. Klaus Fütterer Room 717

Re: [ccp4bb] How it could be possible?

initial MR solution, where you allow each domain to move independently? All the best with this structure. Klaus === Dr. Klaus Fütterer Deputy Head of School Undergraduate Admissions Room 717, Biosciences Tower School

[ccp4bb] Marie Curie Early Stage Research

=== Dr. Klaus Fütterer Deputy Head of School Undergraduate Admissions Room 717, Biosciences Tower School of Biosciences P: +44-(0)-121-414 5895 University of Birmingham F: +44-(0)-121-414 5925 Edgbaston E: k.futte

Re: [ccp4bb] Why nobody comments about the Nobel committee decision?

. Klaus Fütterer Deputy Head of School Undergraduate Admissions Room 717, Biosciences Tower School of Biosciences P: +44-(0)-121-414 5895 University of Birmingham F: +44-(0)-121-414 5925 Edgbaston E: k.futte...@bham.ac.uk

[ccp4bb] Organic solvents for ligand solubilisation

suggestions for alternative organic solvents that have been used to solubilise hydrophobic ligands, and are reasonably gentle to the protein crystal. Thank you. Klaus === Klaus Fütterer, Ph.D

Re: [ccp4bb] delete subject

All, I personally would feel awkward at depositing my hard fought data before the associated paper is accepted. However, a recent paper from our institution (I was not part of that study) originated from precisely the model that has been suggested in this thread:

Re: [ccp4bb] viewing and scoring diffraction using the PX Scanner

'2 cents worth', Klaus === Klaus Fütterer, Ph.D. Reader in Structural Biology Undergraduate Admissions School of Biosciences P: +44-(0)-121-414 5895 University

Re: [ccp4bb] Off Topic: Web or e-tools for booking instrument time

We use Google Calendar for booking our X-ray facility. Klaus === Klaus Fütterer, Ph.D. Reader in Structural Biology Undergraduate Admissions School of Biosciences

Re: [ccp4bb] Lousy diffraction at home but fantastic at the synchrotron?

Marcus, May I ask the following: assuming 8 A is obtained from a single crystal on the home source, what diffraction limit would one expect on the PX scanner? Best regards, Klaus === Klaus Fütterer

Re: [ccp4bb] Difficult to solve MR - A mysterious case

=== Klaus Fütterer, Ph.D. Reader in Structural Biology School of Biosciences P: +44-(0)-121-414 5895 University of Birmingham F: +44-(0)-121-414 5925 Edgbaston E: k.futte...@bham.ac.uk Birmingham, B15 2TT, UK W

Re: [ccp4bb] Refining against images instead of only reflections

the quality of the refined structures significantly. Finding the correct (or best) profile function, however, took a while, at least for the X-ray case. Klaus === Klaus Fütterer, Ph.D. Reader

Re: [ccp4bb] optimizing ncs masks in DM + interpreting output

=== Klaus Fütterer, Ph.D. Reader in Structural Biology School of Biosciences P: +44-(0)-121-414 5895 University of Birmingham F: +44-(0)-121-414 5925 Edgbaston E: k.futte...@bham.ac.uk Birmingham, B15

[ccp4bb] Research Fellow in Structural Biology of Tuberculosis

and crystallization of membrane-bound proteins. Informal enquiries can be addressed to Professor Gurdyal S. Besra by phone (+44 (0)121 415 8125, email: [EMAIL PROTECTED]) or to Dr. Klaus Fütterer (+44 (0) 121 414 5895, [EMAIL PROTECTED]). Maximum starting salary £24,402 a year, in the range

[ccp4bb]

- Klaus Fütterer, Ph.D. School of Biosciences University of Birmingham P: +44-(0)-121-414 5895 Edgbaston, F: +44-(0)-121-414 5925 Birmingham, B15 2TT, UKE: [EMAIL PROTECTED] W