Hi Roger,
I crystallized a protein that started in solution with 250 mM NaCl, 50%
glycerol, and 50 mM Arg+Glu. I initially used traditional microbatch under
paraffin oil to get crystallization hits (where protein and screen are 1:1;
glycerol ends up at 25%). Then, I found that setting up
We've been using Bio-Rad biologic duoflow FPLCs for over 5 years (we have 4
of them on site). They can also handle abuse and cold room storage. Their
hardware and software are user-friendly. It seems the Profinia system is
geared toward simpler purifications.
Okay, I got the problem resolved in the following way (thanks go to Clint
Leysath):
1. removed the tcltk++ directory that came with my ccp4 download
2. installed Activestate's tcltk 8.4.19.2 from
https://www.activestate.com/activetcl/downloads/
3. downloaded blt2.4z.tar.gz and the blt2.4z-patch-2
I am grappling with a linux system for the first time. I am successful in
compiling the source for ccp4 files. When I start ccp4i I see the normal
interface and I can click on the buttons, but the buttons that have menu
lists do not respond to clicks. My terminal reports the following:
*
Thanks! The 75 dpi fonts fixed it!
I am trying to get a current version of ESCET to compare several models of
the same protein with different ligands.
I have tried twice to contact Thomas Schneider at the address listed in this
post:
Hi,
for obtaining ESCET, please contact me directly at:
thomas.schnei...@embl-hamburg.de
Thanks
I'm running flexWarp on a MacBook Pro and on a Linux box. The program goes
through several
cycles and then gives the following errors when trying to improve an MR
solution:
If I start from the PDB file as is
ERROR
Exception class : 'IndexError'
Message : 'no args'
BackTrace (limited to level
Before I place an order for some Izit, are there some other dyes I can use to
check if I've got a protein
crystal?
Thanks,
Mark
At what refinement resolution or resolution ranges would you call a structure
high resolution vs.
low resolution? I realize that this may boil down to semantics (e.g. some
may classify structures as
medium resolution), but I wanted to get an opinion from the pros.
