t; wrote:
>
> Hi Vaheh,
>
> for this purpose, I use
>
> pointless hklin refmacXY.mtz < labin F=FC
> eof
>
> Thus, pointless determines the space group, including the crystallographic
> screw axes, from the Fcalc.
>
> Best wishes,
> Kay
>
>
Hi All,
Interesting discussion as I have a similar case. In my case molecular
replacement solution can be found easily in P21, P212121, with very similar
looking electron densities. However, R-factors remain relatively high (mid
30s). In P1 completeness suffers (75% completeness), maps look
For what it is worth, human serum albumin has been crystallized initially from
250 mg/ml solution back in ‘90s. When I started working on ternary complex
hSA-FcRn-Fc (PDB Id 4N0U) was afraid that such high concentration couldn’t be
achieved with amounts of FcRn I can reasonably express.
Nick,
What files do you mean when call them CCP4 formatted? Coordinates are accepted
only in mmCIF. Experimental data in mtz. What else?
Thank you.
Vaheh
From: CCP4 bulletin board On Behalf Of Nicholas Clark
Sent: Wednesday, January 31, 2024 3:49 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re:
Run SEC on complex prior to crystallization to remove single entities, provided
size difference makes sense. Not too many column options though.
Vaheh
From: CCP4 bulletin board On Behalf Of
careinaedgo...@yahoo.com
Sent: Friday, November 24, 2023 4:00 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re:
Hi Morgan Elizabeth,
In some cases adding 1-2% of cryoprotectant into crystallization drop during
setting those drops up helps to introduce 25-30% of the same cryoprotectant
during harvest, provided you still can get those crystals to grow. Worked for
me in several cases.
Vaheh
From: CCP4
You may as well have fun by manually building your molecule B into 1.9A ed map
when R-factors are already in mid 30s.
Vaheh
From: CCP4 bulletin board On Behalf Of Sam Tang
Sent: Monday, November 6, 2023 2:02 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] RES: [ccp4bb] About model building
Whatever it is worth, I agree with Dale. I see those "Major validation issues"
all the time. Some structures at medium resolution (2-3A), and some are at high
(1.1-1.4A). Not all Arg residues are "in violation". At low and medium
resolution cases it is hard to argue. But high resolution
Hi Rams,
Given the ligand is identical in all structures I'd create and object from just
the ligand and then write a script to align all 50 structures with that ligand
object. I've done that on number of cases.
Hope it works in your case as well.
Vaheh
From: CCP4 bulletin board On Behalf Of
Hi Ben,
All copies created by multiplying cell dimensions will act exactly same as the
original one, mathematically exactly. Nick’s approach is better. Something
similar to what Nick said was published around 2002-2003. I was reviewing it. I
did not understand then what the author was trying
Hi Stephan,
You’re mostly correct, however, gradients made on instrument with one pump are
less reliable.
Thank you.
Vaheh
From: CCP4 bulletin board On Behalf Of Stephan Rempel
Sent: Friday, September 16, 2022 7:38 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] off-topic: experience
Thank you, Paul!
From: CCP4 bulletin board On Behalf Of Paul Emsley
Sent: Friday, July 8, 2022 11:50 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Coot sporadically crashes
Is there an option to save the session automatically every 10-15 minutes or so?
And now to actually answer
Hi All,
Sorry for posting on CCP4BB.
Coot installed together with latest CCP4 on Win10 crashes time to time with no
messages/warnings. Is there an option to save the session automatically every
10-15 minutes or so?
Thank you.
Vaheh
So does PISA with great detailed info on interface residues available.
VO
From: CCP4 bulletin board On Behalf Of Jan Dohnalek
Sent: Tuesday, May 31, 2022 2:48 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] displaying residues (as a surface perhaps) for one
component of a p-p-i
Dear Fred,
Well, there is almost nothing left to suggest, but one. Try co-crystallizing.
While in solution molecules will have more flexibility to adopt a conformation
that fits ligand bound state better. When soaking there might be restrictions
on conformational changes needed for better binding due to
It would probably be safe if you run dialysis first. If you still think that
may harm the expensive SEC column then running PD-10 column first would be
advised.
Hope this helps.
Vaheh Oganesyan, Ph.D.
[cid:image001.png@01D80EB0.8E5CA9A0]
R | Biologics Engineering
One Medimmune Way,
Dear John Helliwell,
I should have been more clear in addressing my email. It was in response to
John Berrisford’s answer.
Regards,
Vaheh
From: John R Helliwell
Sent: Monday, November 1, 2021 3:13 PM
To: Oganesyan, Vaheh
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] renaming chains
Dear
Monday, November 1, 2021 12:53 PM
To: Oganesyan, Vaheh
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] renaming chains
Dear Vaheh
Usually we do not rename chains as part of the curation procedure.
There are instances when we do, for example when a chain has to be split
into two chains and a new cha
Hi All,
This question is mostly for RCSB and PDBe: why are you renaming chains in the
deposited PDB files? Why does it matter what letter is assigned to the chain?
For 1,2 or 3 chain structures it is manageable, but for more chains and/or many
complexes per asu this becomes quite a challenge.
How P3221 can be an option if it assumes chain on axis? I guess I'm missing
something, but per my belief only those sg will be possible for which there is
no axis going through the extra molecule. P1 sg looks the only correct option
here in my humble opinion.
Democracy (voting) depends on
Herman's suggestion is very good. But I'd go one step further and try to grow
crystals in 30-40% PEG400. Then you definitely do not need any additional
cryoprotectant. There is a chance that your crystals are of poor quality to
start with. In that case no cryoprotection will help. You can test
Hi Jan,
They hold nice because of high occupancy or because you have very high
resolution and no restraints are necessary at all (even for protein part)?
Thank you
Vaheh
From: CCP4 bulletin board On Behalf Of Jan Dohnalek
Sent: Tuesday, September 8, 2020 8:01 AM
To: CCP4BB@JISCMAIL.AC.UK
book when new one is coming out.
And I’m not a hoarder, do not have OCD or ADHD.
This is already 4 cents together. Sorry to clutter your mailbox.
Vaheh (CCP4 user since 1992)
From: Christian Roth
Sent: Tuesday, July 7, 2020 12:42 PM
To: Oganesyan, Vaheh
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re
… and how all these changes being justified?
From: CCP4 bulletin board On Behalf Of Eleanor Dodson
Sent: Tuesday, July 7, 2020 12:25 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Accessing full list of programs in CCP4I2
Yes - there are things I use all the time which are not part of the
Hi Chitra Latka,
By far the best approach is to find what protein is interacting with the one
you have the structure and try co-crystallizing them together. At least there
will be some more biology (science) involved in what you will be doing. You may
get lucky and get different packing of
Colleagues,
While on stereo issues here is problem I’m trying to resolve with community
help:
my hardware includes nVidia quadro FX1400, Xpand emiter and NewVision glasses.
Driver taken from nVIDIA site specifically for Quadro FX 1400, long lived. On
old Linux station these worked together fine
Hello Colleagues,
A bit off topic, but could someone point me in the direction of Alwyn’s
executables for “O”?
It doesn’t reside at xray.bmc.uu.se anymore.
Regards,
Vaheh Oganesyan, Ph.D.
Scientist, Biologic Therapeutics
Hi Napo,
Just in case nobody suggested this option I want to add it. Very recently I’ve
solved structure of Fab with 8 molecules per au. It wasn’t easy to do because
some of tNCS were dropped by programs. After contacting the developer of MolRep
Alexey Vagin and asking him to help understand
Try seeding at lower concentration.
From: CCP4 bulletin board On Behalf Of zhangyi19950109
Sent: Friday, March 22, 2019 1:55 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] image
Dear all,
A single plus-stranded RNA virus RDRP monomer grows crystals, precipitants
(PEG8000)
with low
Posting on behalf of colleague (please, do not reply to me):
·
o
§
US - Gaithersburg - MD
· Apply
· Apply with
Firdous,
Many crystallographers (if not all) went through case like you describe. So far
you’ve got great suggestions that are worth trying. I’ll add one more from my
own experience. In all cases I tried it appeared that addition of 0.5 to 1% v/v
of glycerol or ethylene glycol to drop (either
Not very elegant way of doing what you want but as a last resort I used
distance criteria. P-O distance is 1.6A, S-O distance is 1.4A. Provided your
coordinate error is in the range of 0.1A you may cautiously suggest one or the
other. However, it may be impossible to prove that what you see is
Dear crystallographers,
Lately when refining a structure (at 2.8A) with Refmac5 I've found that nearly
all Arg residues get distorted at one angle: NE-CZ-NH1(2). Starting model has
120*, final model 123*(117*), which validation server considers a major issue.
May any of you recognize why is
Hi Herman,
I haven't done His-6 versus His-10 for the same protein, but have done that for
different ones with success. However, if in His-6 containing protein structure
the packing or folding is such that you don't see His-6 then it shouldn't
matter it is 6 or 10. Just an opinion.
Regards,
James,
What you wrote doesn't look like official risk assessment document. However,
your essay is very informative and entertaining. Thank you.
Regards,
Vaheh Oganesyan
www.medimmune.com
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of James
Percent of identity/similarity is a number that might be often misleading when
used as a judgement rule for molecular replacement. Very high or very low
numbers are almost always indicative of corresponding outcome. The numbers in
twilight zone of 20 to 35%, however, are not. When aligning
What I’m about to write should be referred as a question rather than an answer.
However, it might also help to find the answer to crystallization question
discussed here.
The good old crystallization diagram so far for me was something that I’d look
after successful crystallization story and
1:38 PM
To: Oganesyan, Vaheh; Phil Jeffrey; CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Problem with a cell content
Dear Vaheh and Phil,
Sorry, I was a bit misleading – antibody fragment I used is a nanobody (VHH,
15kDa). I was worried about the cell content because when I started the pdb
Похоже, что вы уже решили структуру. Почему вас беспокоит плотность ваших
кристаллов? Кристаллы бывают разные.
First of all Fab by itself is already almost 50 kDa, so complex with antigen
should be more than 50 kDa. Because you already solved the structure calculate
the molecular mass based on
Matthews coef. is not made to give you exact answer about number of molecules
in the asu. As you rightly say, it is just probability.
I’d have been convinced seeing unbiased (before refinement with fifth molecule)
diff. electron density map or discontinuous packing in the crystal in the
absence
Would this figure answer your question? Cells must have way of feeling the
difference in the bond lengths, and strength and nuances in hybridization. At
least, I hope they do.
Regards,
Vaheh Oganesyan
www.medimmune.com
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Colleagues,
Working at relatively high resolution of 1.5A allows in some cases seeing
alternative positions of side chains. After placing those in the coordinate
file with occ adding to 1.0 and running refinement (latest REFMAC on Win) I
find that occupancies of all atoms in the specific
Hi Herman,
While you're correct regarding increase in number of entities in the asu upon
lowering the symmetry, you're not correct for specific case of R32. One
molecule per asu in R32 equals 18 molecules per asu in P1.
Regards,
Vaheh Oganesyan
www.medimmune.com
From: CCP4 bulletin board
a correction.
Next time, I will check the space group before sending an email.
Best regards,
Herman
Von: Oganesyan, Vaheh [mailto:oganesy...@medimmune.com]
Gesendet: Donnerstag, 2. Juli 2015 15:48
An: Schreuder, Herman RD/DE;
CCP4BB@JISCMAIL.AC.UKmailto:CCP4BB@JISCMAIL.AC.UK
Betreff: RE: [ccp4bb
Hi Robbie and Co,
These things are happening now too. Look at the entry 4x4m. The paper got
published in January, PDB released coordinates in April. That means reviewers
did not have a chance to look even at validation report. In my opinion,
whatever it is worth, every journal dealing with
Kay and others,
I think Quadro 5000 available @
http://www.amazon.com/PNY-VCQ5000-PB-DisplayPort-Profesional-Graphics/dp/B003X26T7K
is a better option both price wise and need of transforming the output into
3-pin mini DIN. Having said that I should mention difference: the memory is
2.5GB for
Colleagues,
I’d like to thank everyone who took time to answer my question regarding Quadro
cards that support quad buffered stereo. I now hope to build a workstation with
Quadro 5000.
Regards,
Vaheh Oganesyan
www.medimmune.com
To the extent this electronic communication or any of its
Graphics gurus,
http://www.amazon.com/PNY-DisplayPort-Profesional-Graphics-VCQ6000-PB/dp/B0044XUD1U/ref=sr_1_8?ie=UTF8qid=1427151570sr=8-8keywords=nvidia+quadro
Would this card work for quad buffered stereo on Linux workstation? It does
have 3-pin mini Din.
Regards,
Vaheh Oganesyan
I might be completely wrong here but doesn’t it bother that the number of
rejected reflections shown in log file is ~83000? It shows, at least to me,
that either indexing step is far from being correct or crystal is not really
good enough due to defects.
Vaheh Oganesyan
www.medimmune.com
This fact by itself is unusual to say the least (for me):
we have NO rotational symmetry (2, 3, or 4-fold) whatsoever between
interacting monomers in the ASU or relating those built up by the
crystallographic symmetry
There might be several ways of choosing molecules to represent the asymmetric
Theresa,
Try Genscript. They have probably special now going on, so box of 10 SDS PAGE
costs $39.
I'm not associated with Genscript in any way and I don't use their SDS PAGE.
Vaheh Oganesyan
www.medimmune.com
-Original Message-
From: CCP4 bulletin board
Hi all,
I'm planning migration from CRT monitor to wall mounted screen with projector
that will support stereo. Mid-range emitter like AE125 for small office (4m by
4m) like mine is sufficient. I'm looking for advise on projector and screen. It
looks like ViewSonic PJD7820HD ($699) with
It sounds very interesting: experimental and computational dance biology. Any
type of computational dance or there are style limitations?
Regards,
Vaheh Oganesyan
www.medimmune.com
[MedI Logo Sig]
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Sebastiano Pasqualato
Colleagues,
Sorry to bother for something really minor. The Refmac usually always
recognizes tetrahedral SO4 groups and there were no problems related to its
geometry. Two attached files demonstrate SO4 geometry before and after
refinement. Would you be able to point me to mistake I’m doing?
To: Oganesyan, Vaheh
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] SO4 geometry
On Monday, 31 March, 2014 19:01:33 Oganesyan, Vaheh wrote:
Colleagues,
Sorry to bother for something really minor. The Refmac usually always
recognizes tetrahedral SO4 groups and there were no problems related to its
Colleagues,
I was happily using my NuVision 60GX emitter with Quadro FX1400 graphics card
for number of years on CRT and recently something went bad and image will flip
regularly sending front to back and vice versa. First I thought the card went
bad but after installing new one nothing
Thanks to Debanu Das, Pedro J. B. Pereira and Bernhard Rupp for pointing me in
the right direction.
https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind1308L=ccp4bbP=R2552751=ccp4bb9=AJ=ond=No+Match%3BMatch%3BMatchesz=4
Regards,
Vaheh Oganesyan
www.medimmune.com
To the extent this
Colleagues,
In August of 2013 there was a tread regarding high purity Imidazole and someone
posted list of products with absorption values at 280 nm and manufacturer.
I can find most of the e-mails from that tread but not the one with list of
Imidazoles. Has anyone saved that e-mail for future
It actually looks much like pyrophosphate! If your protein is phosphatase and
the extra density is in vicinity of the active site it might be the remaining
product of reaction.
Vaheh
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
I think this is an advice not to follow.
Vaheh
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Rajiv K
Bedi
Sent: Friday, May 24, 2013 1:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Improve diffraction ...any ideas?
Dear Umri,
Herman,
I don't know which early days you refer to, but from late 80s until structural
genomics era there were relatively few crystallization reports. May be I didn't
see them, and then I apologize. But crystallization reports in large started in
late 90s through early 21st century and Acta F
Hi All,
May I ask opinion of those who currently have standalone UV microscope whether
or not they are happy with their choice? Few that I know are from Formulatrix,
JanScientific and Corima. Pros and cons of your instument are greatly
appreciated.
I'll send the summary to the list.
Number of years ago Jaru Jancarik (the author of Screen I II sold by HR)
while in Berkeley Structural Genomics Center (or may be even earlier) made an
observation regarding protein precipitation in condition A6 in that very
screen. Based on this observation HR sells now PCT (protein
It wasn't doing well lately. So, it was expected.
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Jacob Keller
[j-kell...@fsm.northwestern.edu]
Sent: Thursday, May 31, 2012 2:20 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Death of Rmerge
All,
For those who still don't use Coot is there an automatic water picking
procedure in CCP4?
Many years ago there was a peakmax which is now for Patterson peaks only. Then
there was routine through Arp/wArp. Now is Coot only. Is this right?
Thanks.
Vaheh Oganesyan, PhD
Antibody Discovery
BBers,
In my new 6.2.0 installation I'm getting child killed: segmentation violation
while running Truncate. The /tmp/Vaheh directory does exist and is writable.
Below is the message:
[Vaheh] ===
The program run with command:
I was hesitant to add my opinion so far because I'm used more to listen this
forum rather than tell others what I think.
Why and what to deposit are absolutely interconnected. Once you decide why
you want to do it, then you will probably know what will be the best format and
vice versa.
GE has a policy on Product Obsolescence, which, afaik, means that service
contracts will not be issued to those instruments that were discontinued 7
years ago. Among instruments affected by this deadline are AktaPrime INCL,
AktaPrime EXCL and AktaPrime COMPLETE. You have time to service these
While I believe there is plenty written about metal coordination, the best
approach, IMHO, is to search PDB for metal of your choice at resolution as high
as you can get and compare to your case.
Vaheh
From: CCP4 bulletin board
I completely disagree with Filip's assessment. I've been using nanodrop nearly
5 years and never had inconsistency issues. If you work at reasonable speed (if
you put a drop there then lower the lever and click measure before you do
anything else) there will be no issues. At very high
I think that the absolute value of protein concentration is not very important.
Some proteins get crystallized at 1 mg/ml, others at 50. What is important is
to be able to reproducibly estimate it from prep to prep. You probably want to
start at some reasonable value of about 10 mg/ml. If it in
Using different web servers on your refined structure is good thing to
do. But for distinguishing metal ion, specifically Mg, from water was
done unambiguously before these programs existed. The simple rule is two
fold: 1. distance; 2. coordination. For Mg ion distances are between 2
and 2.2 A and
On the same note with Mirek: does anyone know of a source other than GE for
resin for purification of FLAG-ed proteins?
Thanks.
Vaheh
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Mirek
Cygler
Sent: Tuesday, November 02, 2010 11:47 AM
Thanks go to all who took their time and answered and in some cases did
file manipulations. Extremely helpful! Not only for this particular case
but many times CCP4BB has proven to be the best.
Below are received answers in chronological order:
Albert Gluskov: I converted it in adobe acrobat pro,
Actually it probably would be better ask the supplier of your screen
first how they got PEG 1K diluted to 12.5%. If they did hit it then you
have to do the same. But even better idea is to order that PEG 1K from
the same company that sells the screens. It will insure identical
treatment.
My 2
Dear bb contributors,
I would like to refer to your expertise and get advice regarding
multiple NCS relations between two macromolecular complexes in asu.
Resolution 2.5A, s.g. C2221, R-merge 6%, synchrotron data, 2 complexes
per a.s.u. Each complex consist of three polypeptide chains, let's say
Protein crystals grown in Phosphate-Citrate buffer, pH 4.2 behave exactly the
way Richard described: they first turn blue then fade through yellow-brown.
Vaheh
-Original Message-
From: CCP4 bulletin board on behalf of Richard Gillilan
Sent: Mon 3/15/2010 2:23 PM
To:
Colleagues,
Do any of you have created a def.site file for LRL-CAT 31-ID-D beamline
to be used by HKL2000? I'll appreciate if you can share.
__
Oganesyan Vaheh, Ph.D
Antibody Discovery
MedImmune, Inc.
To the extent this electronic communication or any of its
(with properly chosen resin and buffer pH).
Your idea works fine: that's why I limit load to 300 ml.
Thank you.
___
Vaheh
From: Brad Bennett [mailto:bradbennet...@gmail.com]
Sent: Thursday, October 08, 2009 4:43 PM
To: Oganesyan, Vaheh
Subject: Re
Dear All,
When mammalian cell culture is being loaded to GE HisTrap resin Ni ions are
being stripped off the resin, at least in my hands. Did any of you have similar
experience and if so what kind of work-around was found?
Volume is fairly large (3L) and concentration/dialysis have proven to
In case of cell lysates this may be a good idea since you can adjust the salt
concentration in your sample. In case of secreted proteins it is probably not
so good since the media contains ~150 mM NaCl. In my case this salt prevents
protein from bindinq to Q column.
Thank you anyway.
Colleagues,
Would some one kindly suggest software that calculates shape
complementarity of two interacting proteins based on co-crystal
structure?
I've seen number of reports with sc parameter included but none of
those mention how it was done.
Among non-runnable programs in CCP4 there is the sc
I came across this LCD monitor with DVI connection. Would experts in the
field recommend this monitor for stereo viewing?
http://www.pcconnection.com/IPA/Shop/Product/Detail.htm?sku=6220485
Thank you.
___
Vaheh
To the extent this electronic communication or any of its attachments contain
version of TLSMD. I'm
writing to you to find out if there was any progress in that direction and how
to make carbohydrates to be recognized.
Regards,
Vaheh Oganesyan
___
Vaheh
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Kay Diederichs
Sent
Please, forgive my partially off-topic question.
I'm looking for commercial or cell bank source for GnTI-deficient
HEK293S cells and will appreciate any suggestions on how to get them.
P.S. It's partially off-topic since the cells will be used to produce
protein for crystallography.
Thanks.
is
not required.
To apply, please visit http://www.medimmune.appone.com/ and search by
Req. Number 00616.
__
Oganesyan Vaheh, Ph.D
Antibody Discovery
MedImmune, Inc.
To the extent this electronic communication or any of its attachments contain
information that is not in the public
=-44 RFZ=3.8 TFZ=5.8 PAK=19 LLG=-908
Thank you in advance.
__
Oganesyan Vaheh, Ph.D
Antibody Discovery
MedImmune, Inc.
To the extent this electronic communication or any of its attachments contain
information that is not in the public domain, such information
Mark,
What was the state of the larger drops when tiny counterparts had
crystals? My guess - they all precipitated.
I'm trying to understand why some proteins or some conditions require
change in protein concentration while others do not when migrating from
smaller drops to larger ones. If it
I have been using Phoenix for more than two years and so far there were
no issues with maintenance. Wash the needles and nano-dispenser before
and after the runs and you are good to go.
Electrostatic effects have been seen in a way of drops being positioned
on the side of the flat bottom plate,
Phoebe,
In addition to all other comments I like to add that have had several
cases when crystals would not take/like any cryo agent at all. What I
did is added 1% glycerol in the crystallization solution. That did not
change crystallizability of any of the proteins I worked with, but as a
result
In addition to all good recommendations I would add the following: load
your protein to the whatever-Ni column then wash it with urea. 1 to 4 M
urea will not, in most cases, destroy your protein but will disrupt the
hydrophobic interaction between contaminant and your protein in case if
the
Similar thing was happening on the dual Xeon 2.8 GHz machines in O as well.
But it was 2-5 sec long in time.
Vaheh Oganesyan
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] Behalf Of Kay Diederichs
Sent: Tuesday, August 14, 2007 3:54 PM
To: CCP4BB@JISCMAIL.AC.UK
Isn't this e-mail's topic and it's appearance contradict each other?
It comes from William Scott's e-mail account, but signed as coming from Paul
Emsley.
How do we figure out who actually wrote this e-mail with or without control
access.
The easiest way is to assume that it is coming from Bill,
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