[ccp4bb] dumb software question

, etc measurements with links to the pdb? Thanks in advance. Paul Dr. Paul Kraft Structural Biologist cell 586-596-2770 email: haresea...@yahoo.com

[ccp4bb] before doing an elisa or buying a dye for affinity measurement....

PCR rxn to generate a W mutant would be easy too. Dye detection methods  can be problematic at times, and elisa's have there own problems... Dr. Paul Kraft Structural Biologist cell 586-596-2770 email: haresea...@yahoo.com email: kraft_proteome_resea...@yahoo.com This communication and any

[ccp4bb] linux upgrade preferences for CCP4

hello, I'm considering upgrading my linux software from CENTOS5 to perhaps Fedora or UBUNTO. Does anyone have an opinion about the best linux version to upgrade to for not only CCP4 but also for general robustness and for the best standard apps..Thanks Paul Dr. Paul Kraft Structural Biologist

Re: [ccp4bb] gst-tag protein purify problem

Forgot to add, a 150aa dna binding protein should only need a minimal hexahistidine tagthese proteins are typically very soluble and express well. Adding a GST or sumo tag is overkill. Dr. Paul Kraft Structural Biologist cell 586-596-2770 email: haresea...@yahoo.com email

Re: [ccp4bb] gst-tag protein purify problem

for source the source gene). Paul ps one thing I forgot, you mention it is a DNA binding protein, solublizing in 2M NaCl is much better than 1M NaCl, you could just be losing it...the protein that is :-)   Dr. Paul Kraft Structural Biologist cell 586-596-2770 email: haresea...@yahoo.com email

Re: [ccp4bb] Off Topic: How to delete loops from a protein/homology modeling

with loops spliced in and energy minimized. There are many other more or less complicated programs, but it's a good one to start with. Paul Dr. Paul Kraft Structural Biologist cell 586-596-2770 email: haresea...@yahoo.com email: kraft_proteome_resea...@yahoo.com This communication and any attachments

Re: [ccp4bb] protein aggregation

Try taking the detergents out of your solublization buffer (that is if you know it's not a membrane protein) and only extract the buffer soluble portion. If you have a tag try switching the tag to a different terminus. Dr. Paul Kraft Structural Biologist cell 586-596-2770 email: haresea

[ccp4bb] homology modeling

that can be substituted for CHARM (will GROMOS from SWISS PROT work?).   Also is their any open source homology modeling program that includes solvent interactions in the minimization (it seems they all require $$, at least the good one's). Thanks in advance. Paul Dr. Paul Kraft Structural

Re: [ccp4bb] purification of a strange protein

Dr. Paul Kraft Structural Biologist cell 586-596-2770 email: haresea...@yahoo.com The information constituting this transmission is privileged and confidential. If you are not the intended recipient, please notify me immediately. --- On Tue, 6/8/10, Oliver Li lihua...@hotmail.com wrote: From

[ccp4bb] Fw: Re: [ccp4bb] Structural importance of ordered water?

waters in crystal structures vs NMR structures? Paul Kraft --- On Tue, 6/17/08, Sanishvili, Ruslan [EMAIL PROTECTED] wrote: From: Sanishvili, Ruslan [EMAIL PROTECTED] Subject: Re: [ccp4bb] Structural importance of ordered water? To: CCP4BB@JISCMAIL.AC.UK Date: Tuesday, June 17, 2008, 5:16 PM Hi

[ccp4bb] libstdc++.so.5: cannot open shared object file: No such file or directory

shared object file: No such file or directory for pretty much all of the progrrams. I know this is a library problem I've seen before but can't remember how to fix it. Your help would be appreciated. Thanks, Paul Dr. Paul Kraft Structural Biology Research Associate Dept. Biochemistry and Molecular

[ccp4bb] ipmosflm centos4.4 mouse enlarge doesn't work

crappy data and the first indexing puts the spots slightly off and you need to adjust them. Any ideas besides switching to an older version of either centos or CCP4? Best Regards, Paul Dr. Paul Kraft Structural Biology Research Associate Dept. Biochemistry and Molecular Biology Laboratory of Dr