Re: [ccp4bb] Help with preparation of the ligand coordinates/restraints needed

Dear CCP4 Users, Thank you very much for all suggestions. Special thanks to Oliver for the ready-to-use files. Great job :) Hopefully it will solve my problems... Best wishes, Rafal -- |--| |Rafal Dolot, Ph.D

Re: [ccp4bb] Help with preparation of the ligand coordinates/restraints needed

'm wrong. Have you any idea, where is a problem? Best, Rafal --- |--| |Rafal Dolot, Ph.D.| | | |Polish Academy of Sciences| |Centre of Molecular and Macromolecular Studies|

[ccp4bb] Help with preparation of the ligand coordinates/restraints needed

to prepare a new ligand like this one, for incorporation into the protein and further refinement? Best regards, Rafal Dolot -- |--| |Rafal Dolot, Ph.D.| | | |Polish Academy

Re: [ccp4bb] Problems with refinement of nucleic acid structure

Dear all, Thank you for the response. I will try to explain it more precisely. The molecule of interest is a duplex with 9 nt length, but is paired on the length of eight bases, with overhangs at ends. Molecules form a parallel strings across the crystal lattice, parallel to C-axis, because

Re: [ccp4bb] According correct space group assignment...

regards, Rafal --- |--| |Rafal Dolot, Ph.D.| | | |Polish Academy of Sciences| |Centre of Molecular and Macromolecular Studies| |Department of Bioorganic Chemistry

[ccp4bb] According correct space group assignment...

-tetraplex formation. Data were collected to almost 1 A, so it should be enough for trials with direct methods/ab initio solution. What I should do first to find correct SG and/or cell parameters? Best regards, Rafal -- |--| |Rafal Dolot, Ph.D

[ccp4bb] Another problem after last CCP4 update...

Dear CCP4 users, I observed problems with "Real space refine zone" in Coot after CCP4 package last update, which revealed a complete disaster in phosphate groups of nucleotides... Windows 8.1 system Best regards, Rafal -- |------| |Rafal D

[ccp4bb] Dyes for DNA crystals

advices, Rafal Dolot |--| |Rafal Dolot, Ph.D.| | | |Polish Academy of Sciences| |Centre of Molecular and Macromolecular Studies| |Department of Bioorganic

[ccp4bb] Problem with aimless/ctruncate

XDSdataset has failed with error message child killed: segmentation violation *** When I use old truncate, it works fine. Best regards Rafal |--| |Rafal Dolot, Ph.D

[ccp4bb] Diffraction image viewer with display of resolution circles

- maybe detector format is a problem? Best regards, Rafal |--| |Rafal Dolot, Ph.D.| | | |Polish Academy of Sciences| |Centre of Molecular and Macromolecular

[ccp4bb] Question about stereo on LCD monitor

. With best wishes Rafal |--| |Rafal Dolot, Ph.D.| | | |Polish Academy of Sciences| |Centre of Molecular and Macromolecular Studies| |Department of Bioorganic

[ccp4bb] Software for RNA model building

protein chain building, but without options of DNA/RNA chain building. With best wishes Rafal |--| |Rafal Dolot, Ph.D.| | | |Polish Academy of Sciences| |Centre

Re: [ccp4bb] Question about stereo on LCD monitor

wishes Rafal |--| |Rafal Dolot, Ph.D.| | | |Polish Academy of Sciences| |Centre of Molecular and Macromolecular Studies| |Department of Bioorganic Chemistry

[ccp4bb] Question about stereo on LCD monitor

card with shutterglasses, but with CRT Iiyama monitor. Maybe after 2-3 years something changes in this subject... With best wishes Rafal Rafal Dolot, Ph.D Polish Academy of Sciences Centre of Molecular and Macromolecular Studies Department of Bioorganic Chemistry