[ccp4bb] Molrep and Refmac5 errors

2013-10-15 Thread Rajiv K Bedi
Dear all, I recently started using CCP4i (Windows 8) software for data processing and model building but I get the following error messages whenever I use molrep and refmac5: MOLREP #CCP4I TERMINATION STATUS 0 MOLREP(ccp4): Error in label assignments in LABIN #CCP4I TERMINATION TIME 08 Oct

Re: [ccp4bb] Improve diffraction ...any ideas?

2013-05-24 Thread Rajiv K Bedi
I think you can soak another protein into a protein crystal if it is small enough to pass through water channels, I guess. More info: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483499/

Re: [ccp4bb] Improve diffraction ...any ideas?

2013-05-23 Thread Rajiv K Bedi
Dear Umri, I think the main problem is co-crystallization. What I would do is crystallize protein and antibody separately and then soak protein crystals into reservoir solution containing antibody or vice versa. And do try to get crystals from different conditions which may alter the space