ACA BioSAS Training Workshop WK.01
Biological Small Angle Solution Scattering
Theory and Practice
2013 ACA meeting in Hawaii
There is still Room. Reserve your space now! The early registration discount
ends May 31*.
Small angle solution scattering (SAS) has recently experienced a dramatic
I am looking for easily-available proteins that exist in equilibrium between
two or more known oligomeric states
in solution.
BSA is a possibility, but I am concerned that the rate constant for association
may be rather slow. Fast exchange would be better.
Does anyone know of other
BioSAXS time is available at CHESS
Please apply soon to guarantee your spot!
Dear BioSAXS users,
Beamtime is available for the Summer run (May 8 - June 18, 2013) for BioSAXS.
To apply for time, please submit an online express-mode proposal at
This has been a point of confusion
Here are the conventions used in a few of the classic SAXS text vs. recent
reviews:
--
I believe Guinier actually used the variable u in his thesis, but must have
changed at some point (I don't have it handy to check at the moment).
Guinier
Three years is starting to be a reasonable time interval to see interesting
scientific progress. On the 1 or 2 years time scale it is hard, as anyone
organizing annual meetings will tell you.
One thing I would do is to browse the abstracts of the most recent American
Crystallographic
Hi Chelsy, yes we had a lot of trouble with the nanoview during that run. Even
after going through the calibration procedure with the special fluid provided,
we still had inconsistent results even on standards. Finally, I carefully
cleaned the return light path of the instrument (a separate
There are actually a bunch of depth cues that humans use to perceive
3-dimensionality. Existing computer displays only reproduce a few. Because of
redundancy, people can function with only a subset, but it can be a serious
handicap. Individuals who are stereoblind can learn to judge distances
This has been a problem for us too. Sorry, I don't have a solution to offer
except, recently, we have provided metal buckets filled with a layer of
aquarium gravel at each station and encouraged users to dump their excess
nitrogen there instead of on the floor.
Richard
On Feb 16, 2011, at
Does anyone know of a water soluble dye that changes color upon exposure to
x-rays?
Preferably from clear to a dark color. Must work in the liquid state
(non-frozen... so color centers are out).
Doesn't matter if it is organic or inorganic.
Thanks
Richard Gillilan
MacCHESS
Cornell
Since several people have asked me for code, I now realize that I've
contributed my bit to the confusion over names of various surface definitions.
To clear things up there is an excellent online article which covers the
history of various molecular surface definitions that I highly recommend:
-nancy.frhttp://medecine.uhp-nancy.fr
Cell.: +33 (0)6.11.35.69.09
On 13/01/2011 13:40, Richard Edward Gillilan wrote:
:
Subject: Re: [ccp4bb] What is the simplest method to analytically compute the
Solvent-Accessible Surface Area of a given atom in a protein?
My knowledge on this is probably
:
Subject: Re: [ccp4bb] What is the simplest method to analytically compute the
Solvent-Accessible Surface Area of a given atom in a protein?
My knowledge on this is probably quite out of date by now, but some years ago
there was a lot of research on this topic because such surfaces are
What kind of SAXS data are you looking for?
Richard
On Jan 11, 2011, at 11:29 PM, Rojan Shrestha wrote:
Hello:
I am searching for SAXS data depositor like protein data bank for protein
structure and EDS from electron density map. I have no idea how can I get SAXS
data. Is there a depository
I will second this particular reference (below). A number of our SAXS users
have found it to be one of the most accessible and practical guides for the
non-specialist/non-physicist, with lots of emphasis on biological applications.
Someone already mentioned the EMBO practical course web link,
Small angle x-ray solution scattering (SAXS) can also give you molecular
weight, though not quite as accurately as the best static light scattering.
While SAXS is preferably done on monodisperse systems extrapolated to infinite
dilution, cases in which the monomer and dimer are in equilibrium
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