Re: [ccp4bb] Off-topic: coomassie linearity?

microg per band or spot. Staining and destaining require only 30 min, and the method is compatible with subsequent immunodetection. PMID: 16336940 Regards, Steve -- Steven Darnell University of Wisconsin-Madison Madison, WI USA Jacob Keller said the following, on 09/25/2008 04:35 PM: Dear

Re: [ccp4bb] interface energetics

the following on 9/12/08 4:42 AM: Thanks to all who responded to my question regarding the energetics of a known interface applied to orthogolous dimers. Steven Darnell asked me for some clarifications. I have the structure of a homodimer, defined the dimerization interface and substituted

Re: [ccp4bb] interface energetics

suggestions or corrections? I've only had 7 hours of sleep since Saturday. ~Steve -- Steven Darnell Univeristy of Wisconsin-Madison Madison, WI USA [EMAIL PROTECTED]

Re: [ccp4bb] Off-topic: Native gel electrophoresis of basic proteins

this gel system, we show that the polypyrimidine tract-binding protein (PTB) is a monomer. PMID: 16991206 [PubMed - indexed for MEDLINE] -- Steven Darnell Department of Biochemistry University of Wisconsin-Madison Madison, WI USA [EMAIL PROTECTED] said the following on 8/26/08 12:20 PM: You could

Re: [ccp4bb] interface

interface area. Potentially confusing. Phil Jeffrey Princeton -- Steven Darnell Department of Biochemistry University of Wisconsin-Madison Madison, WI USA

Re: [ccp4bb] interface

Sorry, that equation should read: Buried_Surface_Area = ASA_unbound1 + ASA_unbound2 - ASA_bound ASA = Accessible Surface Area The way I wrote it before would give you a negative value. Regards, Steve Darnell -- Steven Darnell Department of Biochemistry University of Wisconsin-Madison Madison