Hello all,
Looking for advice from any CCP4MG users.
I am making some structural figures and have run into a problem. I posted a
question to the CCP4MG-specific email list quite a while ago but received no
responses, so I don't know if that list is still active.
Essentially, I want to change
I want to second the recommendation to try microseed matrix screening. I
recently had a case of a protein that did not yield any crystals after trying
more than 500 conditions. Of those 500, one single condition gave to me what
appeared to be crystalline material, but not distinct single
ical Biology
University of Pittsburgh School of Medicine
From: Ethan A Merritt<mailto:merr...@uw.edu>
Sent: Thursday, February 27, 2020 6:57 PM
To: Whitley, Matthew J<mailto:mjw...@pitt.edu>
Cc: CCP4BB@jiscmail.ac.uk<mailto:CCP4BB@jiscmail.ac.uk>
Subject: Re: [ccp4bb] Hydrogens in PDB
Hello all,
I am nearly finished refining the structures of two mutant proteins from
crystals that diffracted to very high resolution, 1 Å and 1.2 Å, respectively.
Refinement was conducted in the presence of explicit hydrogens on the models.
I am preparing to deposit these models into the PDB
In the 2019 book Wilhelm Conrad Röntgen: The Birth of Radiology by Gerd
Rosenbusch and Annemarie de Knecht-van Eekelen, there is a table of all the
doctoral and/or habilitation students supervised by Röntgen.
In this table, Walter Friedrich's dissertation is dated 24 July 1911 and
carries the
Hi Tiantian,
You say you have a 'co-crystal' of your protein with the magic triangle, but
how do you really know that I3C is there? The fact that you put it into the
crystallization drop doesn't necessarily mean that it made it into the crystal.
In fact, based on the output of shelxc that
Greetings all,
We have solved a small molecule structure using electron diffraction and would
like to refine the structure with SHELXL. Coming from a macromolecular
background, this is our first experience with SHELXL, and we are not exactly
sure how to proceed. The first thing to do seems
r 27, 2018 5:41 AM
To: Whitley, Matthew J
Cc: ccp4bb
Subject: Re: [ccp4bb] Off topic: 'Difficult' Datasets for Processing Practice
Dear Matthew,
I am also late in responding to this, but as part of a
Nature Protocols paper on iMosflm (Supplementary Information for Na
For some reason, the September 19th ccp4bb digest got caught in my spam filter
and didn't come through until a few minutes ago, so I didn't see several
responses concerning interesting datasets for processing until just now.
Therefore, thanks also to Kay Diederichs, Eugene Osipov, and David
later in the fall.
Sincerely,
Matthew
---
Matthew J. Whitley, Ph.D.
Research Instructor
Department of Pharmacology & Chemical Biology
University of Pittsburgh School of Medicine
On 9/19/2018 5:15 PM, Whitley, Matthew J wrote:
Dear colleagues,
For teaching purposes, I am looking for a s
Dear colleagues,
For teaching purposes, I am looking for a small number (< 5) of
macromolecular diffraction datasets (raw images) that might be
considered 'difficult' for a beginning crystallography student to
process. By 'difficult' I generally mean not able to be processed
automatically by
I would also be interested in learning whether polyoxotungstate has
worked wonders for anyone. At the current price of 387 EUR/gram, it is
a bit too expensive for us to seriously consider giving it a try. Yes,
a gram is a lot of compound and would probably last a long time, but
there are
not active, aborted
The path to the ccp4mg executable on our system is as follows:
/usr/local/ccp4-7.0/ccp4-7.0/bin/ccp4mg
I would appreciate any ideas about the source of the issue and how to resolve
it.
Sincerely,
Matthew Whitley
---
Matthew J. Whitley, Ph.D.
Research Instructor
W. Furey Lab
I second (or third) the suggestion that others have given to try soaking
mercury compounds into your crystal. Mercury absolutely loves free cysteines,
and if you have 5, you have a great chance of getting binding. If isomorphism
is maintained after soaking, the isomorphous differences will be
Hi Claire,
Isn’t the simplest answer just that the EDS server is calculating the
completeness for a different amount of data (high res limit of 1.92 Å), whereas
in your Depositor data it was only calculated to a high res limit of 2.1 Å?
This would probably be the result of you measuring
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