Re: [ccp4bb] Modeling Disulfide Bond Occupancies

2024-05-06 Thread Jeroen Mesters
Do not know what the resolution is here, try an anisotropic refinement of the Cys side chains first to see if this solves your problem. __ Dr. math. et dis. nat. Jeroen R. Mesters University of Lübeck https://orcid.org/-0001-8532-6699 Am 06.05.2024 um 21:48 schrieb Eleanor Dodson

Re: [ccp4bb] To Trim or Not to To Trim

2023-03-17 Thread mesters@biochem
Dear all, to trim or not to to trim…. - what is the difference between highly disordered side-chain atoms versus disordered bulk-solvent atoms? Do not both represent a continuum function with a comparable electron-density distribution? Have not solvent and side-chain atoms merged/mixed at

Re: [ccp4bb] Rational mutations and crystallizability

2023-03-08 Thread mesters@biochem
R. Mesters https://orcid.org/-0001-8532-6699 University of Lübeck Institute of Biochemistry https://www.biochem.uni-luebeck.de phone: +49-451-3101-3105 Ratzeburger Allee 160 23562 Lübeck Germany -- > Am 08.03.2023 um 13:42 schrieb mesters@biochem > : > > > https://servic

Re: [ccp4bb] Rational mutations and crystallizability

2023-03-08 Thread mesters@biochem
https://services.mbi.ucla.edu/SER/intro.php <https://services.mbi.ucla.edu/SER/intro.php> Best J. -- Dr. math. et dis. nat. Jeroen R. Mesters https://orcid.org/-0001-8532-6699 University of Lübeck Institute of Biochemistry https://www.biochem.uni-luebeck.de phone: +49-451-310

Re: [ccp4bb] NMR structires

2023-03-02 Thread mesters@biochem
Maybe intensional to stress the word „paradoxal“ included in the title of the deposition? Same could be true for the related entry 1HIS to stress the word „dynamics“? I guess back in in 1993-1994, structures were not checked rigorously… Jeroen -- Dr. math. et dis. nat. Jeroen R. Mesters https

Re: [ccp4bb] complementarity between the protein and the ligand/compound

2023-02-12 Thread mesters@biochem
Could this be what you are looking for? http://biosig.unimelb.edu.au/csm_lig/prediction <http://biosig.unimelb.edu.au/csm_lig/prediction> J. -- Dr. math. et dis. nat. Jeroen R. Mesters https://orcid.org/-0001-8532-6699 University of Lübeck Germany -- > Am 12.02.2023 um 03:34 sc

Re: [ccp4bb] 3d stereo on windows 10/11

2023-02-10 Thread mesters@biochem
Active stereo support is slowly dying and the hardware will fail in the end (sorry to say)…. One possible alternative is a passive stereo PluraView , which is expensive but compatible with software such as YASARA,

Re: [ccp4bb] Ice Lamellar formation over the MicroTube - Oxford cryo stream 700

2023-02-09 Thread mesters@biochem
luck, J. -- Dr. math. et dis. nat. Jeroen R. Mesters https://orcid.org/-0001-8532-6699 University of Lübeck Institute of Biochemistry https://www.biochem.uni-luebeck.de phone: +49-451-3101-3105 Ratzeburger Allee 160 23562 Lübeck Germany -- > Am 09.02.2023 um 09:14 schrieb Natesh Ramanat

Re: [ccp4bb] Regarding the diffraction image

2023-02-03 Thread mesters@biochem
. et dis. nat. Jeroen R. Mesters https://orcid.org/-0001-8532-6699 University of Lübeck Institute of Biochemistry https://www.biochem.uni-luebeck.de phone: +49-451-3101-3105 Ratzeburger Allee 160 23562 Lübeck Germany -- > Am 03.02.2023 um 09:22 schrieb kavyashreem : > > Dear

[ccp4bb] FEBS Advanced Course - Biomolecules in action III - Hamburg - June 19–24, 2022

2022-05-03 Thread mesters
worthy data with whichever bioanalytical technique applied. A multidisciplinary blend of lectures and practical exercises will allow the participants to interact closely with expert tutors to maximize training outcome. Organizers: Jeroen Mesters, Christian Betzel *Final deadline for application:

Re: [ccp4bb] Ligand occupancy refinement

2022-03-03 Thread mesters
To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1> -- signature.html *Dr. /math. et dis. nat./ Jeroen R. Mesters *Deputy, Lecturer, Program Coo

[ccp4bb] FEBS Advanced Course - Biomolecules in action III - Hamburg - June 19–24, 2022

2022-02-24 Thread mesters
worthy data with whichever bioanalytical technique applied. A multidisciplinary blend of lectures and practical exercises will allow the participants to interact closely with expert tutors to maximize training outcome. Organizers: Jeroen Mesters, Christian Betzel *Deadline for applications: YTFs,

Re: [ccp4bb] Strategies for increasing Trp content of proteins / Tris-NTA-Orange-Green

2022-02-19 Thread mesters
B list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1> -- signature.html *Dr. /math. et dis. nat./ Jeroen R. Mesters *Deputy, Lecturer, Program Coordinator Infection Biology

Re: [ccp4bb] Validation of structure prediction

2021-12-20 Thread mesters
B=1> To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1> -- signature.html *Dr. /math. et dis. nat./ Jeroen R. Mesters *Depu

Re: [ccp4bb] [ccpem] relion benchmark with recent GPUs

2021-03-26 Thread mesters
ve Bus speed (1 Go/s) be enough to "feed" all the GPU without slowing down the system ? Is extra PCI-Express card with SSD at 3 Go/s a preferred choice for this kind of node ? Any feedback is welcome Pierre-Damien Coureux -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, L

Re: [ccp4bb] Maniatis: Molecular Cloning

2021-03-26 Thread mesters
rock "Molecular Cloning" are still being printed and sold? I could not find it by ISBN (0‐87969‐309‐6) at my favourite book store. Or is there anything comparable? Best regards, Tim -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, Lecturer, Program Coordinator /Infection Biology / &

[ccp4bb] Fwd: [ccp4bb] Co-crystallization and thermal shift assay

2021-02-25 Thread mesters
s worth using/testing), but whether the complex can in fact be co-crystallized (the ultimate "test"!).  So yes, go ahead and test it. Best, Jeroen -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, Lecturer, Program Coordinator /Infection Biology / <http://www.uni-luebeck.de/studi

Re: [ccp4bb] Relion's own implementation of motioncorr2 on gpu cluster

2020-09-11 Thread mesters
n should I use? To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, Lecturer, Program Coordinator /Infection Bi

Re: [ccp4bb] protein oligomer

2020-07-20 Thread mesters
ning. Thanks! Best Regards To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, Lecturer,

Re: [ccp4bb] A question of density

2020-03-04 Thread mesters
CCP4BB=1 -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, Lecturer, Program Coordinator /Infection Biology / <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html>Visiting Professorship (South Bohemian University) in Biophysics Logo Uni Lübeck *Universit

Re: [ccp4bb] Are there any proteins capable of crystallizing at a wide range of pH having the same space group?

2019-09-20 Thread mesters
you know any proteins overpopulating the PDB (i.e. besides HEWL)? Lots of thanks as always. Murphy To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1

Re: [ccp4bb] Secrets of ZYMIT

2019-09-13 Thread mesters
scmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, Lecturer, Program Coordinator /Infection Biology / <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html>Visiting Professorship (South Bohemian University) in Bi

[ccp4bb] FEBS advanced/practical course "Biomolecules in Action II", Hamburg, June 23-28, 2019

2019-03-12 Thread mesters
on2019.febsevents.org Poster:https://biomolaction2019.febsevents.org/download/PC19-044_Poster.pdf Course organizers: Christian Betzel (Hamburg University) and Jeroen Mesters (Lübeck University) To unsubscribe from the CCP4B

Re: [ccp4bb] SO4 or PO4

2019-02-16 Thread mesters
badmin?SUBED1=CCP4BB=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, Lecturer, Program Coordinator /Infection Biology / <

Re: [ccp4bb] Crystallization problems using MPD (2-Methyl-2,4-pentanediol)

2019-02-02 Thread mesters
) -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, Lecturer, Program Coordinator /Infection Biology / <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html>Visiting Professorship (South Bohemian University) in Biophysics Logo Uni Lübeck *University of

[ccp4bb] FEBS advanced/practical course "Biomolecules in Action II", Hamburg, June 23-28, 2019

2019-01-28 Thread mesters
on2019.febsevents.org Poster: https://biomolaction2019.febsevents.org/download/PC19-044_Poster.pdf Course organizers: Christian Betzel (Hamburg University) and Jeroen Mesters (Lübeck University) ** To unsubscrib

Re: [ccp4bb] How to calculate Molecular weight of protein_reg

2018-12-28 Thread mesters
nd Regards To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 -- *Dr.math. et dis. nat.Jeroen R. Mesters* Deputy, Lecturer, Program Coordinator /Infection Biology / <http://www.uni-luebeck.de/studium/studiengaenge/infe

Re: [ccp4bb] CHAPS provider

2018-11-30 Thread mesters
n/webadmin?SUBED1=CCP4BB=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher

Re: [ccp4bb] suggestions for cryoprotectant

2018-10-20 Thread mesters
Thanks Firdous To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.

Re: [ccp4bb] [Offtopic] Protein stain in Coomassie Blue dyes

2018-10-05 Thread mesters
://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB=1 -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html> Institute of Biochemistry,

Re: [ccp4bb] Reference for hanging drop crystallization

2018-03-17 Thread mesters
race the origins of the technique, or a particularly thorough textbook or review? Shane Caldwell shane.caldwel...@gmail.com <mailto:shane.caldwel...@gmail.com> -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ &

Re: [ccp4bb] "Atomic resolution"

2018-01-12 Thread mesters
/04/00/jm5025/index.html> and Responses to/`Atomic resolution': a badly abused term in structural biology / <https://doi.org/10.1107/S205979831700417X> <https://doi.org/10.1107/S205979831700417X> <https://doi.org/10.1107/S205979831700417X> / /- J. - -- Dr.math. et dis. nat

Re: [ccp4bb] Super protein wire model bender

2018-01-09 Thread mesters
05 008 -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html> Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538

Re: [ccp4bb] 3D stereo and pymol

2017-11-28 Thread mesters
sorry, small correction, it should read "or a "new, see details" Quadro 5000with build-in*3-pin**.*" Am 28.11.17 um 15:40 schrieb mesters: Hi, yes, consumer cards do have OpenGL implemented on a chip but the Nvidia driver did not allow OpenGL 3D to work until February

Re: [ccp4bb] 3D stereo and pymol

2017-11-28 Thread mesters
ly support openGL, but posts from October last year on the BB suggests that it only works in windows unless you have a monitor with a built in emitter. I would appreciate any feedback. Regards Christine -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researche

Re: [ccp4bb] long loop

2017-07-05 Thread mesters
to me to get crystal. Would be very grateful for any advice! Thanks Dong Xiao -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introducti

Re: [ccp4bb] ATP analog

2017-05-03 Thread mesters
. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html> Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-45

Re: [ccp4bb] Using a codon-optimised gene to improve proteinsolubility

2017-04-04 Thread mesters
. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html> Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-45

Re: [ccp4bb] protein precipitation reg

2017-03-30 Thread mesters
are ranked. Happy reading, Jeroen Am 30.03.17 um 23:36 schrieb Edward A. Berry: On 03/30/2017 08:10 AM, mesters wrote: If the pI of the protein is below the pH of the buffer (net negatively charged protein), optimum stabilization (salting out; lower solubility) of the macromolecule

Re: [ccp4bb] protein precipitation reg

2017-03-30 Thread mesters
ards Akila -- Akilandeswari G -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html> Institute of Biochemistry, University of Lübeck Ratzebur

Re: [ccp4bb] Coot and pymol 3D for Centos 7?

2017-02-01 Thread mesters
but I could not make it work under Centos 7. WinCoot was not able to load big virus maps, it crashed all the time. I really want to make coot 3D work under Centos 7. Has anybody succeeded at running coot 3D in Centos 7? Jun -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher &

Re: [ccp4bb] Need suggestion for protein solubility

2016-12-25 Thread mesters
0mM NaCl, 10% Glycerol. Elution buffer has varying concentration of imidazole varying from 10mM to 300mM. Any kind of suggestion will be highly appreciated. My project requires structure determination. Thanks in advance. Regards Praveen -- Dr.math. et dis. nat. Jeroen R. Mesters De

Re: [ccp4bb] 3D viewing room options

2016-11-14 Thread mesters
2, I didn't try it on linux). I tested wincoot, pymol, chimera and Amira in stereo, which all worked. Matt -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-bio

Re: [ccp4bb] Good 3D Monitor for Molecular Modelling

2016-10-31 Thread mesters
ist, but am on the hunt for someone who is. All help appreciated. Kind regards, Matt -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.ht

Re: [ccp4bb] Crystals grow at the bottom of the tube.

2016-10-19 Thread mesters
-- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher & Lecturer Program Coordinator /Infection Biology/ <http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html> Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübec

Re: [ccp4bb] OT: looking for a 3D-beamer that works with nvidia nvision

2015-06-11 Thread mesters
-witte http://grk1721.genzentrum.lmu.de/gregor-witte -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher Lecturer Program Coordinator /Infection Biology/ http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html Institute of Biochemistry

Re: [ccp4bb] nVidia quadro Update for Linux

2015-04-02 Thread mesters
: On 04/02/2015 02:05 PM, mesters wrote: After Kay send me an email today to have a look at the wiki at http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Stereo to add my findings, I researched things further. I remember I wrote to the ccp4bb March 1st, 2013, asking about Nvidia 3D Vision

[ccp4bb] [SUSPECTED SPAM] Re: [ccp4bb] nVidia quadro Update for Linux

2015-04-02 Thread mesters
withdraw my previous email. Jeroen Am 02.04.15 um 17:34 schrieb mesters: Be aware though, the option 3-pin + USB for Nvidia 3D Vision 2 glasses is not running/supported under Linux! For linux boxes and Nvidia 3D Vision 2 glasses: You have to buy an 120Hz ASUS VG287HR (there is no other useful

Re: [ccp4bb] nVidia quadro

2015-04-02 Thread mesters
. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher Lecturer Program Coordinator /Infection Biology/ http://www.uni-luebeck.de/studium/studiengaenge/infection-biology/introduction.html Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49

Re: [ccp4bb] nVidia quadro

2015-03-24 Thread mesters
dis. nat. Jeroen R. Mesters Deputy, Senior Researcher Lecturer Coordinator master's program Infection Biology Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-451-5004065 (secretariate -5004061) fax: +49-451-5004068 http://www.biochem.uni

Re: [ccp4bb] Graphics cards for Coot, Pymol, Chimera on MacBook Pro Laptop

2015-03-18 Thread mesters
with me? Thanks a lot for your answer. Best wishes, Marc -- Dr.math. et dis. nat. Jeroen R. Mesters Deputy, Senior Researcher Lecturer Coordinator master's program Infection Biology Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-451

Re: [ccp4bb] non-specific disulfide bonds in crystal structures

2014-12-21 Thread mesters
, but I'm looking for some info on a lazy saturday. Thanks in advance. Best- Todd -- Dr.Jeroen R. Mesters Deputy, Senior Researcher Lecturer Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-451-5004065 (secretariate 5004061) fax: +49-451

Re: [ccp4bb] ICCBM15 workshop completely full / overbooked

2014-07-23 Thread mesters
. Chairpersons: Christian Betzel and Jeroen Mesters

[ccp4bb] Gentle reminder, early Bird Registration deadline ICCBM15 in Hamburg

2014-07-08 Thread mesters
, so better sign up sooner than later. Chairpersons: Christian Betzel and Jeroen Mesters

[ccp4bb] How to grow crystals for serial crystallography, neutron diffraction and other exciting techniques

2014-06-03 Thread mesters
later. Chairpersons: Christian Betzel and Jeroen Mesters

[ccp4bb] Sep. 17-20, Hamburg - Crystallization for novel radiation sources - ICCBM15

2014-05-02 Thread mesters
Betzel and Jeroen Mesters

Re: [ccp4bb] Stereo monitors for use with Pymol and Coot

2014-03-06 Thread mesters
Dr. Jeroen R. Mesters Deputy, Senior Researcher Lecturer Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-451-5004065 (secretariate 5004061) fax: +49-451-5004068 http://

Re: [ccp4bb] Room temperature data collection

2014-02-06 Thread mesters
ctors like Pilatus useful for this? Thank you. Theresa -- Dr. Jeroen R. Mesters Deputy and Group Leader Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-4

[ccp4bb] Save the date: ICCBM15, Sep. 17-20, Hamburg - Crystallization for novel radiation sources.

2014-01-03 Thread mesters
electron microscopy The conference is preceded by a hands-on crystallization workshop (Sep. 14-16) Chairpersons: Christian Betzel and Jeroen Mesters 2014: International Year of Crystallography -- Dr. Jeroen R. Mesters

Re: [ccp4bb] small crystals

2013-12-05 Thread mesters
: Check Authenticity -- Dr. Jeroen R. Mesters Deputy and Group Leader Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-451-5004065 (secretariate 5004061

Re: [ccp4bb] small crystals

2013-12-05 Thread mesters
Hi, sorry, it should read salt in not inverse salt in ... - J. - Am 05.12.13 19:55, schrieb mesters: Hi, Showers of crystals often occur if the protein is not that soluble/happy/stable in the solute. The solubility of a protein depends on its concentration, its pI, pH of solute

Re: [ccp4bb] Stereo monitor

2013-11-26 Thread mesters
h/people/tobias ___ -- Dr. Jeroen R. Mesters Deputy and Group Leader Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-451-5004065 (secretariate 5004061) fax: +49-451-5004068

Re: [ccp4bb] 3D stereo notebook

2013-11-23 Thread mesters
it under linux as well. - J. - -- Dr. Jeroen R. Mesters Deputy and Group Leader Institute of Biochemistry, University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany phone: +49-451-5004065 (secretariate 5004061

Re: [ccp4bb] 3D Monitors

2013-01-27 Thread mesters
://www.computerbase.de/forum/showthread.php?t=949874 - J. - -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538 Lübeck Tel: +49-451-5004065 Fax: +49

[ccp4bb] DLP Link vs. IR emitter based 3D projection

2012-06-14 Thread mesters
Hi, I am looking for an affordable 3D Protein Cave configuration (thus, no quadro 1500 US$ and no 3D stereoscopic projector 1500 US$). At Schrödinger (Pymol) they successfully operate a viewsonic pro8450w, a high-end quadro and the vnidia 3D vision ready glasses (USB port based IR emitter)

[ccp4bb] ICCBM14 Workshop Conference, Hunstville, Alabama

2012-06-13 Thread mesters
We cordially invite you to participate in the 14th International Conference on the Crystallization of Biological Macromolecules (ICCBM14) on September 23-28, 2012. The conference will be held in Huntsville, the /Rocket City/, in the beautiful state of Alabama. The meeting site will be at the

[ccp4bb] coot-0.5.2-osx-universal.dmg.gz

2010-10-22 Thread Jeroen Mesters
I am looking for coot-0.5.2-osx-universal.dmg.gz Anybody out there that can help? - J. -

[ccp4bb] Positions to be filled in Lübeck

2010-05-12 Thread mesters
resumé, including the names of two possible referees, before Mai 31, 2010, indicating the respective reference number, to Universität zu Lübeck – Dezernat Personal – Ratzeburger Allee 160, 23538 Lübeck, Germany -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese

Re: [ccp4bb] Alternatives to ChemDraw 3D? Yes, Chemdoodle!

2010-02-08 Thread mesters
Science Engineering 2-2 Wakamatsu-cho, Shinjuku-ku Tokyo, Japan 162-8480 tel. 03-5369-7369 (ext. 3241) fax. 03-5367-2820 ~ -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck

Re: [ccp4bb] Stereo TF - VGA to 3-pin VESA Stereo Adapter

2010-01-19 Thread mesters
. - J. - Sabuj Pattanayek wrote: On Mon, Jan 18, 2010 at 7:33 AM, mesters mest...@biochem.uni-luebeck.de wrote: Hi, the following information might be of use for the ones that do not have a 3-pin mini connector and are working under linux http://www.int03.co.uk/crema/hardware/stereo

Re: [ccp4bb] Stereo worked, but can't log back on after the screen lock Re: [ccp4bb] [COOT] 120hz LCDs and coot stereo

2010-01-19 Thread mesters
will not work with coot. But i think the reason is that the drivers for these non-quadro cards do not support true quad buffered stereo. -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck

Re: [ccp4bb] Stereo TF - VGA to 3-pin VESA Stereo Adapter

2010-01-18 Thread mesters
: http://forums.nvidia.com/index.php?showtopic=91072st=0p=968627#entry968627 HTH, Sabuj Pattanayek -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger

[ccp4bb] coot-0.5.2-osx-universal.dmg.gz problem

2009-11-23 Thread mesters
the stage at which one is asked to enter the administration password.. The installation of ccp4mg-universal-1.1.2.dmg worked. Jeroen. -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische

Re: [ccp4bb] video that explains, very simply, what Structural Molecular Biology is about

2009-11-16 Thread mesters
biochemical data! - J. - -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538 Lübeck Tel: +49-451-5004065, Fax: +49-451-5004068 Http

Re: [ccp4bb] Test of cryoconditions without X-rays or cryostream

2009-09-22 Thread mesters
/middleeast/windows/windowslive/products/photo-gallery-edit.aspx -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538 Lübeck Tel: +49-451

[ccp4bb] Job openings for doctoral candidates and postal doctoral candidates in structural virology

2009-09-18 Thread mesters
At the Institute of Biochemistry of the University of Lübeck (Germany), job openings for *doctoral candidates and postal doctoral candidates in structural virology * are available to reinforce the ongoing research projects: 1. Postal-doctoral candidate in crystallography / structural virology

Re: [ccp4bb] Lost my protein

2009-05-05 Thread mesters
of the Amicon tube ate my protein. Why this happen and how to recover my protein? Thank you very much. Yan -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie

Re: [ccp4bb] Permanent industrial position for a PhD crystallographer in Munich, southern Germany

2009-04-08 Thread mesters
. ** -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538 Lübeck Tel: +49-451-5004065, Fax: +49-451-5004068 Http

[ccp4bb] friction

2009-04-02 Thread mesters
, the deposition in general has not become any simpler or faster. Or, has it? -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538 Lübeck Tel: +49

Re: [ccp4bb] sticky crystals

2009-01-27 Thread mesters
/spyware-doctor-antivirus/ http://www.pctools.com/en/spyware-doctor-antivirus/ * -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538

Re: [ccp4bb] structure (factor) amplitude

2009-01-12 Thread mesters
of Arizona 520 621 8171 s...@email.arizona.edu mailto:s...@email.arizona.edu http://www.biochem.arizona.edu/xray -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und

Re: [ccp4bb] 2D

2009-01-07 Thread mesters
! - J. - Vin Purp wrote: Sorry for the non ccp4 related post. Can anyone point me in the direction of a good method, reference or link for improving 2D xtals? They are hexagonal 2D plates, and some have a tendency to stack. Cheers. =v= -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle

Re: [ccp4bb] Fwd: [ccp4bb] X-Ray versus NMR Structure

2008-11-16 Thread mesters
. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538 Lübeck Tel: +49-451-5004065, Fax: +49-451-5004068 Http://www.biochem.uni-luebeck.de Http://www.iobcr.org

Re: [ccp4bb] Crystal growing in one direction...need suggestions

2008-10-14 Thread mesters
, Detergent kit from Hampton and lot more alongwith temperature variation. Need suggestions regarding improvement of xtal quality, THICKNESS. Thanx in advance. Shivesh -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu

Re: [ccp4bb] Progresss with Stereo 3D under Mac OS X Leopard

2008-09-17 Thread mesters
for your time. -- Dr. Jeroen R. Mesters Gruppenleiter Strukturelle Neurobiologie und Kristallogenese Institut für Biochemie, Universität zu Lübeck Zentrum für Medizinische Struktur- und Zellbiologie Ratzeburger Allee 160, D-23538 Lübeck Tel: +49-451-5004070, Fax: +49-451-5004068 Http

Re: [ccp4bb] small crystallisation incubator

2008-04-17 Thread mesters
my bench (in a genetics lab) and hold a few dozen 96 and some 24 well plates. A friend already suggested to get a wine-cooler?! I would be grateful for some advice. Thanks! Michael -- Jeroen Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische

[ccp4bb] [Fwd: Re: Summary of Thermofluor]

2008-03-31 Thread mesters
be interesting for anybody intending to buy a new machine. - J. - Original Message Subject:Re: Summary of Thermofluor Date: Thu, 14 Feb 2008 12:05:08 +0100 From: Jeroen Mesters [EMAIL PROTECTED] To: [EMAIL PROTECTED] References: [EMAIL PROTECTED] mesters

[ccp4bb] practical protein crystallization courses

2008-02-15 Thread mesters
If You are interested in practical protein crystallization courses, have a look at the following link: http://www.iobcr.org/science.html J. -- Jeroen Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische Struktur und Zellbiologie Ratzeburger Allee

Re: [ccp4bb] Summary of Thermofluor

2008-02-14 Thread Jeroen Mesters
mesters wrote: Sorry for the off-topic but can somebody recommend highly a sensitive RT-PCR machine for the thermofluor experiment (sypro orange). That would imply excitation below 500 nm (ideally 470) and detection at about 570 nm, right? I know several simple machines have a problem

Re: [ccp4bb] Thermofluor experiment

2008-02-13 Thread mesters
Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [EMAIL PROTECTED] Tel -49 7071 601 323 Fax -49 7071 601 349 -- Jeroen Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische Struktur und

Re: [ccp4bb] Thermofluor

2008-02-13 Thread mesters
think this is the least expensive RT-PCR on the market but it performs very well in our experiments. It can excite the samples between 470 and 505 nm and one of the detection channel covers the Sypro Orange emission. The control software is VERY easy to use. Andrzej, HY mesters wrote: Sorry

[ccp4bb] Job opportunities in Luebeck

2008-02-13 Thread mesters
the Director of the Institute of Biochemistry, Prof. Rolf Hilgenfeld, Tel. 0451-500-4060; [EMAIL PROTECTED] -- Jeroen Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische Struktur und Zellbiologie Ratzeburger Allee 160, D-23538 Luebeck Tel: +49-451

[ccp4bb] Thermofluor

2008-02-12 Thread mesters
... Jeroen. -- Jeroen Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische Struktur und Zellbiologie Ratzeburger Allee 160, D-23538 Luebeck Tel: +49-451-5004070, Fax: +49-451-5004068 E-mail: [EMAIL PROTECTED] Http://www.biochem.uni-luebeck.de Http

Re: [ccp4bb] 3D Glasses - Infocus DepthQ

2008-02-06 Thread mesters
that does stereo in CRT work the same ? Is there an 'emmiter' or not ? Do you know how much glasses (and anything else extra) cost ? Thanks a lot - Tassos -- Jeroen Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische Struktur und Zellbiologie

Re: [ccp4bb] WG: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

2008-02-04 Thread Jeroen Mesters
Hi Gregor, I think this LCD monitor is not useful since the refresh rate is only 75 Hz, thus in stereo 2 x 37.5 Hz and that is going to give a big headache. I know Samsung is working on a 100 Hz LCD-TV. Nevertheless, the goal is not for stereo but to suppress the afterglow effects again. They

[ccp4bb] ICCBM12 in Cancun, Mexico

2008-01-07 Thread mesters
, please visit http://www.iccbm12.com.mx/ See you there! Jeroen. -- Jeroen Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische Struktur und Zellbiologie Ratzeburger Allee 160, D-23538 Luebeck Tel: +49-451-5004070, Fax: +49-451-5004068 E-mail: [EMAIL

Re: [ccp4bb] protein degradation?

2007-11-05 Thread Jeroen Mesters
Hi, if I recall this correctly, it is the nickel that is in your sample after elution and boiling your protein in SDS sample buffer does the rest. So, could be the sample is fully okay!!! J. Tiago Botelho wrote: Hi, I also had a similar problem with one of my proteins... I had it cloned

Re: [ccp4bb] Is phophorylation possible in E. coli expression system?

2007-10-29 Thread mesters
Tu is phosphorylated in vivo. J Biol Chem. 1993 Jan 5;268(1):601-7. Alexander C, Bilgin N, Lindschau C, Mesters JR, Kraal B, Hilgenfeld R, Erdmann VA, Lippmann C. Related Articles, Links Phosphorylation of elongation factor

Re: [ccp4bb] Cost-effective imaging systems: recommendations and opinions requested

2007-10-29 Thread mesters
is very welcome as well, as is any information about the associated software. I’ll compile and re-post all comments and opinions for everyone else who might find this information useful. Thanks very much in advance, Rebecca Rebecca Page Brown University -- Jeroen Raymundus Mesters, Ph.D

Re: [ccp4bb] extra density on Cysteine

2007-08-14 Thread mesters
Raymundus Mesters, Ph.D. Institut fuer Biochemie, Universitaet zu Luebeck Zentrum fuer Medizinische Struktur und Zellbiologie Ratzeburger Allee 160, D-23538 Luebeck Tel: +49-451-5004070, Fax: +49-451-5004068 E-mail: [EMAIL PROTECTED] Http://www.biochem.uni-luebeck.de Http://www.iobcr.org Http

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