Re: [ccp4bb] How to determine the concentration of biotinylated peptide?

These suggestions are all possible, but why not simply lyophilize it into a tared tube and weigh it out? On Mon, Feb 6, 2017 at 12:28 PM, Alex Lee wrote: > Thank you all for your suggestions! > > On Mon, Feb 6, 2017 at 5:53 AM, Artem Evdokimov

Re: [ccp4bb] How to determine the concentration of biotinylated peptide?

Hi, In addition to HABA dye assay (which will work great but will also be fooled by any biotin that is not conjugated) you can do: * quantitative MS * TLC * HPLC * elemental analysis * https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614710/ biotin catalysis of the N3- + I3- reaction (also fooled

Re: [ccp4bb] How to determine the concentration of biotinylated peptide?

Hi Alex, In addition to Mirella's suggestion I would like to make an addition which might be specifically useful for you. Since your peptide has biotin tag, You may use HABA dye assay for the exact quatifiation of biotin (and thus biotinylated peptide). As far I recall, Thermo scientific

Re: [ccp4bb] How to determine the concentration of biotinylated peptide?

Hi Alex, you can measure the absorbance at 214-220 nm, which is where the peptide bonds absorb, but you should know/calculate/predict the extinction coefficient of your peptide at that wavelength. Furthermore, you might try BCA assay which is colorimetric as the bradford but the reaction

[ccp4bb] How to determine the concentration of biotinylated peptide?

Dear All, Sorry for the off-topic question, I'd like to do Biacore SPR assay with N-terminal biotinylated peptide as ligand (to Biacore SA chip) and my protein as analyte. I have a question of how to determine the concentration of biotinylated peptide （synthetic peptide), if the peptide has no