Re: [ccp4bb] ITC question -dimer vs monomer

ober 4, 2019 5:06:10 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] ITC question -dimer vs monomer Thanks to All for the extended & informative responses. If true thermodynamic equilibria are realized, then I would agree that regardless of the pathway the endpoint (or integrated

Re: [ccp4bb] ITC question -dimer vs monomer

m. I may keep bugging selected victims off-board once I have the first data. Many thanks again, BR From: CCP4 bulletin board On Behalf Of Barone, Matthias Sent: Thursday, October 3, 2019 18:59 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] ITC question -dimer vs monomer As Reza alr

Re: [ccp4bb] ITC question -dimer vs monomer

Rupp" À: CCP4BB@JISCMAIL.AC.UK Envoyé: Jeudi 3 Octobre 2019 17:05:56 Objet: [ccp4bb] ITC question -dimer vs monomer Hi Fellows, please let me ask the respective experts an ITC question: I have 2 proteins, stable and dialyzed in identical buffer. A is a monomer and B an obligate dimer. I s

Re: [ccp4bb] ITC question -dimer vs monomer

93-284 From: CCP4 bulletin board on behalf of Roger Rowlett Sent: Thursday, October 3, 2019 8:36:27 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] ITC question -dimer vs monomer Won't this depend on the relative final concentrations of A and B in the two experiments? If A

Re: [ccp4bb] ITC question -dimer vs monomer

Thursday, October 3, 2019 17:41 > *To:* CCP4BB@JISCMAIL.AC.UK > *Subject:* Re: [ccp4bb] ITC question -dimer vs monomer > > > > I don’t understand what you are trying to do—are you trying to show, by > the difference in ITC response, that the pre

Re: [ccp4bb] ITC question -dimer vs monomer

so try with SPR. Always best to do these things > using multiple complimentary methods > > > > Best wishes > > > > Clare > > > > *From:* CCP4 bulletin board *On Behalf Of *Bernhard > Rupp > *Sent:* 03 October 2019 16:06 > *To:* CCP4BB@JISCMAIL.AC.UK

Re: [ccp4bb] ITC question -dimer vs monomer

.com/url?sa=t=web=j=http://www.chem.gla.ac.uk/staff/alanc/itcdil.pdf=2ahUKEwiOpoeisYDlAhUDThUIHX5mAyMQFjAAegQIAhAB=AOvVaw0Ng-W03upy4DG12unFGDY3 From: CCP4 bulletin board On Behalf Of Bernhard Rupp Sent: 03 October 2019 16:52 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] ITC question -dimer vs monomer I am not

Re: [ccp4bb] ITC question -dimer vs monomer

2019 16:06 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] ITC question -dimer vs monomer Hi Fellows, please let me ask the respective experts an ITC question: I have 2 proteins, stable and dialyzed in identical buffer. A is a monomer and B an obligate dimer. I suspect that eventually a A2B2 dimer

Re: [ccp4bb] ITC question -dimer vs monomer

/itcdil.pdf=2ahUKEwiOpoeisYDlAhUDThUIHX5mAyMQFjAAegQIAhAB=AOvVaw0Ng-W03upy4DG12unFGDY3 From: CCP4 bulletin board On Behalf Of Bernhard Rupp Sent: 03 October 2019 16:52 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] ITC question -dimer vs monomer I am not looking for anything yet - I wonder what

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] ITC question -dimer vs monomer

York Department of Chemistry New York, NY 10031 From: CCP4 bulletin board on behalf of Bernhard Rupp Sent: Thursday, October 3, 2019 11:51 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [EXTERNAL] Re: [ccp4bb] ITC question -dimer vs monomer I am not looking for anything

Re: [ccp4bb] ITC question -dimer vs monomer

To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] ITC question -dimer vs monomer I don't understand what you are trying to do-are you trying to show, by the difference in ITC response, that the predictions you made about the oligomerization are true? JPK

Re: [ccp4bb] ITC question -dimer vs monomer

: Thursday, October 3, 2019 11:06 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] ITC question -dimer vs monomer Hi Fellows, please let me ask the respective experts an ITC question: I have 2 proteins, stable and dialyzed in identical buffer. A is a monomer and B an obligate dimer. I suspect

[ccp4bb] ITC question -dimer vs monomer

Hi Fellows, please let me ask the respective experts an ITC question: I have 2 proteins, stable and dialyzed in identical buffer. A is a monomer and B an obligate dimer. I suspect that eventually a A2B2 dimer will form. Intuitively, it should make a difference whether I titrate the dimer

Re: [ccp4bb] ITC question

I recall a case of this a few years ago where it had to do with the relative concentration of the protein to the buffer/salt molecules (can't remember which anymore), which ended up being important in the binding that was observed. So it was entirely a concentration effect that caused the

Re: [ccp4bb] ITC question

Le Vendredi 17 Mars 2017 16:07 CET, Nicholas Larsen a écrit: Do you mean that the affinity from ITC is 100-fold weaker ? Which would mean that the Kd values from ITC are 100-fold larger (in the range 0.1-1 µM) ? If this is the case, it makes me think to a

[ccp4bb] ITC question

Dear colleagues, We have a target where people have measured Kd's for ligands using radioligand binding assays. Several publications report Kd's of single digit nanomolar and we are able to reproduce that data using this assay format. When we try to do the same measurement using ITC, we generate