Dear Matthew,
at your resolution, 1.7A, you are seeing a large difference in
resolution limits. I'd be curious to know how much "anisotropy" you have
in the single number anisoB. Could you share it?
As to building felxible loops or peptide, I suspect you anisoB to be
mild (<40A2 for your
Dear Matthew,
You seem to have been unlucky with this data collection. STARANISO
would be prepared to see significant data extending to 2.0A along a*, 2.7A
along b*, and 1.7A along c*. The latter limit, however, involves some guess
work via the fitting by an ellipsoid of a cut-off surface
*Please* dont throw good 1.8A data for the sake of statistics!
You should see more detail along certain directions
You will publish your structure providing honest details of the anisotropy
(I hope..) but it is the map quality that matters ..
Eleanor
On Sun, 21 Jun 2020 at 15:16, Matthew Snee <
Hi everyone.
I have an x ray structure that I am finishing up, and there are a few ambiguous
regions where the peptide is poorly resolved.
The data is highly anisotrophic, and requires truncation to around 2.4A to
achieve acceptable merging stats, although there is data in the "good"
